Genetic Transformation of Medicago Species

2003 ◽  
pp. 223-237
Author(s):  
Ray J. Rose ◽  
Kim E. Nolan ◽  
Chen Niu
Keyword(s):  
Genetic Transformation ◽  
Medicago Species

Genetic transformation of Medicago species by Agrobacterium tumefaciens and electroporation of protoplasts

1990 ◽  
Vol 8 (11) ◽  
pp. 660-663 ◽  
Author(s):  
Nicolay Kuchuk ◽  
Igor Komarnitski ◽  
Anatoliy Shakhovsky ◽  
Yuri Gleba
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Genetic Transformation ◽  
Medicago Species

Stable genetic transformation of Arabidopsis thaliana by Agrobacterium inoculation in planta

1994 ◽  
Vol 5 (4) ◽  
pp. 551-558 ◽  
Author(s):  
Seok So Chang ◽  
Soon Ki Park ◽  
Byung Chul Kim ◽  
Bong Joong Kang ◽  
Dal Ung Kim ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Genetic Transformation ◽  
In Planta

Genetic transformation of Ginkgo biloba by Agrobacterium tumefaciens

2000 ◽  
Vol 108 (4) ◽  
pp. 413-419
Author(s):  
Patricia Dupré ◽  
Jerôme Lacoux ◽  
Godfrey Neutelings ◽  
Dominique Mattar-Laurain ◽  
Marc-André Fliniaux ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Genetic Transformation ◽  
Ginkgo Biloba

Construction of expression vectors of the melon resistance gene Fom-2 and genetic transformation

2018 ◽  
Vol 51 (1) ◽  
Author(s):  
Xiaomei Li ◽  
Liu Liu ◽  
Xiangyu Liu ◽  
Yan Hou ◽  
Bingyin Xu ◽  
...  
Keyword(s):  
Genetic Transformation ◽  
Resistance Gene ◽  
Expression Vectors

RNA Interference Vector Construction, Genetic Transformation and Function Analysis of Rice Transcription FactorOsMYB14

2013 ◽  
Vol 19 (6) ◽  
pp. 960
Author(s):  
Yanwen MIAO ◽  
Rong LI ◽  
Fangjie XIONG ◽  
Zhengming ZENG ◽  
Xiangli NIU ◽  
...  
Keyword(s):  
Rna Interference ◽  
Genetic Transformation ◽  
Function Analysis ◽  
Vector Construction ◽  
And Function

Construction and Genetic Transformation of TomatoARF4RNA Interference Expression Vector with Fruit Specific Promoter

2012 ◽  
Vol 18 (2) ◽  
pp. 206
Author(s):  
Yuanyuan FENG ◽  
Yingnan LI ◽  
Pei HOU ◽  
Jingrong ZHANG ◽  
Songhu WANG ◽  
...  
Keyword(s):  
Genetic Transformation ◽  
Expression Vector

Genetic transformation of commercial breeding lines of alfalfa (Medicago sativa)

1995 ◽  
Vol 42 (2) ◽  
pp. 129-140 ◽  
Author(s):  
R�jean Desgagn�s ◽  
Serge Laberge ◽  
Guy Allard ◽  
Habib Khoudi ◽  
Yves Castonguay ◽  
...  
Keyword(s):  
Genetic Transformation ◽  
Medicago Sativa ◽  
Breeding Lines ◽  
Commercial Breeding

scholarly journals Efficient CRISPR/Cas9 mediated Pooled-sgRNAs assembly accelerates targeting multiple genes related to male sterility in cotton

Plant Methods ◽  
2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Mohamed Ramadan ◽  
Muna Alariqi ◽  
Yizan Ma ◽  
Yanlong Li ◽  
Zhenping Liu ◽  
...  
Keyword(s):  
Genetic Transformation ◽  
Sequence Similarity ◽  
High Specificity ◽  
Transformation Method ◽  
High Sequence Similarity ◽  
Single Experiment ◽  
Next Generation Sequencing Technology ◽  
Cotton Transformation ◽  
Assembly Method ◽  
Multiple Genes

Abstract Background Upland cotton (Gossypium hirsutum), harboring a complex allotetraploid genome, consists of A and D sub-genomes. Every gene has multiple copies with high sequence similarity that makes genetic, genomic and functional analyses extremely challenging. The recent accessibility of CRISPR/Cas9 tool provides the ability to modify targeted locus efficiently in various complicated plant genomes. However, current cotton transformation method targeting one gene requires a complicated, long and laborious regeneration process. Hence, optimizing strategy that targeting multiple genes is of great value in cotton functional genomics and genetic engineering. Results To target multiple genes in a single experiment, 112 plant development-related genes were knocked out via optimized CRISPR/Cas9 system. We optimized the key steps of pooled sgRNAs assembly method by which 116 sgRNAs pooled together into 4 groups (each group consisted of 29 sgRNAs). Each group of sgRNAs was compiled in one PCR reaction which subsequently went through one round of vector construction, transformation, sgRNAs identification and also one round of genetic transformation. Through the genetic transformation mediated Agrobacterium, we successfully generated more than 800 plants. For mutants identification, Next Generation Sequencing technology has been used and results showed that all generated plants were positive and all targeted genes were covered. Interestingly, among all the transgenic plants, 85% harbored a single sgRNA insertion, 9% two insertions, 3% three different sgRNAs insertions, 2.5% mutated sgRNAs. These plants with different targeted sgRNAs exhibited numerous combinations of phenotypes in plant flowering tissues. Conclusion All targeted genes were successfully edited with high specificity. Our pooled sgRNAs assembly offers a simple, fast and efficient method/strategy to target multiple genes in one time and surely accelerated the study of genes function in cotton.

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