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2022 ◽  
Vol 9 ◽  
Author(s):  
Huaxu Zhou ◽  
Yao Ding ◽  
Ruobing Su ◽  
Dongming Lu ◽  
Hongliang Tang ◽  
...  

Convenient and sensitive detection of active analytes in complex matrix is crucial in biological, medical, and environmental analysis. Silica nanochannel array film (SNF) equipped electrochemical sensors have shown excellent anti-fouling performance in direct analysis of complex samples. In this work, we demonstrated an electrochemical sensor with anti-fouling performance for highly sensitive detection of acetaminophen (APAP) based on SNF supported by ß-cyclodextrin-graphene (CDG) nanocomposite modified Au film electrode (AuF). Because of their rich surface hydroxyls and 2D lamellar structure, CDG on AuF can serve as the nanoadhesive for compact binding SNF, which can be grown by electrochemical assisted self-assembly method in a few seconds. Attributable to the electrocatalytic property of graphene and the synergistic enrichment from both CD and SNF nanochannels towards analyte, the SNF/CDG/AuF sensor demonstrates sensitive detection of acetaminophen ranged from 0.2 to 50 μM with an ultralow limit-of-detection of 14 nM. Taking advantage of the anti-fouling ability of SNF, the sensor is able to realize accurate and convenient analysis of APAP in commercially available paracetamol tablets.


2022 ◽  
Author(s):  
Haiyang Wu ◽  
Yubin Yuan ◽  
Qiang Wu ◽  
Xiangrui Bu ◽  
Long Hu ◽  
...  

Abstract In order to meet the requirements of ultra-fast real-time monitoring of sarin simulator with high sensitivity and selectivity, it is of great significance to develop high performance dimethyl methylphonate (DMMP) sensor. Herein, we proposed a DMMP sensor based on p-hexafluoroisopropanol phenyl (HFIPPH) modified self-assembled single-walled carbon nanotubes (SWCNTs) with field effect transistor (FET) structure. The self-assembly method provides a 4 nanometres thick and micron sized SWCNT channel, with high selectivity to DMMP. The proposed SWCNTs-HFIPPH based sensor exhibits remarkably higher response to DMMP than bare SWCNT based gas sensor within only few seconds. The gas sensing response of SWCNTs-HFIPPH based sensor for 1ppm DMMP is 18.2%, and the response time is about 10 seconds. What's more, the gas sensor we proposed here shows excellent selectivity and reproducibility, and the limitation of detection is as low as ppb level. The proposed method lays the foundation for miniaturization and integration of DMMP sensors, expecting to develop detection system for practical sarin sensing application.


2022 ◽  
Author(s):  
Matthew C Haines ◽  
Benedict Carling ◽  
James Marshall ◽  
Marko Storch ◽  
Paul C Freemont

Standardized DNA assembly methods utilizing modular components provide a powerful framework to explore design spaces and iterate through Design-Build-Test-Learn cycles. Biopart Assembly Standard for Idempotent Cloning (BASIC) DNA assembly uses modular parts and linkers, is highly accurate, easy to automate, free for academic and commercial use, while enabling simple hierarchical assemblies through an idempotent format. These attributes facilitate various applications including pathway engineering, ribosome binding site tuning, fusion protein synthesis and multiplex gRNA expression. In this work we present basicsynbio, an open-source software encompassing a Web App (https://basicsynbio.web.app/) and Python Package (https://github.com/LondonBiofoundry/basicsynbio). With basicsynbio, users can access commonly used BASIC parts and linkers while robustly designing new parts and assemblies with exception handling for common design errors. Furthermore, users can export sequence data and create build instructions for manual or automated workflows. The generation of build instructions relies on the BasicBuild Open Standard which is easily parsed for bespoke workflows and is serialised in Java Script Object Notation for transfer and storage. We demonstrate basicsynbio by assembling a collection of 30 BASIC-compatible vectors using various sequences including modules from the Standard European Vector Architecture (SEVA). The BASIC SEVA collection encompasses plasmids containing six antibiotic resistance markers and five origins of replication from different compatibility groups, including a temperature-sensitive variant. We deposit the collection on Addgene under an OpenMTA agreement, making them available. Furthermore, these sequences are accessible from within the basicsynbio application programming interface along with other collections of parts and linkers, providing an ideal environment to design BASIC DNA assemblies for bioengineering applications.


Molecules ◽  
2022 ◽  
Vol 27 (1) ◽  
pp. 270
Author(s):  
Xu Li ◽  
Hua Shi ◽  
Yunhan Gu ◽  
Qingyan Cheng ◽  
Yanji Wang

A citric acid ligand assisted self-assembly method is used for the synthesis of ternary mesoporous cerium lanthanum solid solution doped with metal elements (Co, Zr, Mg). Their textural property was characterized by X-ray diffraction, transmission electron microscopy, N2 adsorption-desorption, X-ray photoelectron spectroscopy and TPD techniques, and so on. The results of catalytic testing for synthesis of dimethyl carbonate (DMC) from CH3OH and CO2 indicated that the DMC yield reached 316 mmol/g on Ce-La-Co solid solution when the reaction temperature was 413 K and the reaction pressure was 8.0 MPa. It was found that Co had synergistic effect with La and Ce, doping of Co on the mesoporous Ce-La solid solution was helpful to increase the surface area of the catalyst, promote CO2 adsorption and activation, and improve the redox performance of solid solution catalyst. The conversion of Co2+ to Co3+ resulted in the continuous redox cycle between Ce4+ and Ce3+, and the oxygen vacancy content of the catalyst was increased. Studies have shown that the catalytic performance of Ce-La-Co solid solution is positively correlated with oxygen vacancy content. On this basis, the reaction mechanism of DMC synthesis from CO2 and CH3OH on the catalyst was speculated.


2022 ◽  
Author(s):  
Behnam Enghiad ◽  
Pu Xue ◽  
Nilmani Singh ◽  
Aashutosh Girish Boob ◽  
Chengyou Shi ◽  
...  

Plasmids are used extensively in basic and applied biology. However, design and construction of plasmids, specifically the ones carrying complex genetic information, remains one of the most time-consuming, labor-intensive, and rate-limiting steps in performing sophisticated biological experiments. Here, we report the development of a versatile, robust, automated end-to-end platform named PlasmidMaker that allows error-free construction of plasmids with virtually any sequences in a high-throughput manner. This platform consists of a most versatile DNA assembly method using Pyrococcus furiosus Argonaute (PfAgo)-based artificial restriction enzymes, a user-friendly frontend for plasmid design, and a backend that streamlines the workflow and integration with a robotic system. As a proof of concept, we used this platform to generate 101 plasmids from six different species ranging from 5 to 18 kb in size from up to 11 DNA fragments within 3 days. PlasmidMaker should greatly expand the potential of synthetic biology.


Soft Matter ◽  
2022 ◽  
Author(s):  
Xiangqian Gao ◽  
Tiantian Deng ◽  
Xindi Huang ◽  
Mengmeng Yu ◽  
Danyang Li ◽  
...  

A new composite hydrogels with excellent self-healing properties was prepared by combining poly(vinyl alcohol) (PVA) and boron nitride nanofibers (BNNFs) via a facile one-pot assembly method. One-dimensional porous BNNFs with...


2021 ◽  
Author(s):  
Julie Vanderstraeten ◽  
Maria João Maurício da Fonseca ◽  
Philippe De Groote ◽  
Dennis Grimon ◽  
Hans Gerstmans ◽  
...  

Abstract Background: Designer cellulosomes are self-assembled chimeric enzyme complexes that can be used to improve lignocellulosic biomass degradation. They are composed of a synthetic multimodular backbone protein, termed the scaffoldin, and a range of different chimeric docking enzymes that degrade polysaccharides. Over the years, several functional designer cellulosomes have been constructed. Since many parameters influence the efficiency of these multi-enzyme complexes, there is a need to optimise designer cellulosome architecture by testing combinatorial arrangements of docking enzyme and scaffoldin variants. However, the modular cloning procedures are tedious and cumbersome. Results: VersaTile is a combinatorial DNA assembly method, allowing the rapid construction and thus comparison of a range of modular proteins. Here, we present the extension of the VersaTile platform to facilitate the construction of designer cellulosomes. We have constructed a tile repository, composed of dockerins, cohesins, linkers, tags and enzymatically active modules. The developed toolbox allows us to efficiently create and optimise designer cellulosomes at an unprecedented speed. As a proof of concept, a trivalent designer cellulosome able to degrade the specific hemicellulose substrate, galactomannan, was constructed and optimised. The main factors influencing cellulosome efficiency were found to be the selected dockerins and linkers and the docking enzyme ratio on the scaffoldin. The optimised designer cellulosome was able to hydrolyse the galactomannan polysaccharide and release mannose and galactose monomers. Conclusion: We have eliminated one of the main technical hurdles in the designer cellulosome field and anticipate the VersaTile platform to be a starting point in the development of more elaborate multi-enzyme complexes.


2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Zeyuan Sun ◽  
Hong Yang ◽  
Que Dong ◽  
Yang Mo ◽  
Hui Li ◽  
...  

Using space stations for a large number of observation, exploration, and research is a necessary way to fully develop space technology. It is a necessary means of space experiment to install the extravehicular experimental load by using the load plate. However, the extravehicular environment is full of danger, which poses a threat to the health and even safety of astronauts. Using robots to replace astronauts to complete this task can effectively reduce the threat to astronauts. Aiming at the problem that the configurations of existing space robots have difficulty in balancing the contradiction between complexity and dexterity, our previous work proposes a 12-DOF 3-arm robot and preliminarily explores the feasibility of its large-scale ability. This paper focus on the 8-DOF redundant dexterous manipulator composed of 2 of the robot arms. In view of the difficulties in solving the inverse kinematics of the redundant manipulator, the challenges of complex environmental lighting, and difficulties of matching multiple groups of holes and pins in the load plate assembly task, the research on the autonomous assembly of the load plate is carried out. The main work is as follows: (a) A variable D-H parameter inverse kinematics solution method is proposed, which lays a foundation for humanoid dexterous operation planning of the robot. (b) An autonomous operation method based on visual guidance and variable parameter admittance control is proposed. Finally, the safety and robustness of the robot in the autonomous assembly of the load plate with multipins and holes are successfully verified by experiments.


2021 ◽  
Vol 9 ◽  
Author(s):  
Dongsheng Li ◽  
Bichen Zhu ◽  
Zhongti Sun ◽  
Qinqin Liu ◽  
Lele Wang ◽  
...  

One of the basic challenges of CO2 photoreduction is to develop efficient photocatalysts, and the construction of heterostructure photocatalysts with intimate interfaces is an effective strategy to enhance interfacial charge transfer for realizing high photocatalytic activity. Herein, a novel UiO-66/Bi4O5Br2 heterostructure photocatalyst was constructed by depositing UiO-66 nanoparticles with octahedral morphology over the Bi4O5Br2 nanoflowers assembled from the Bi4O5Br2 nanosheets via an electrostatic self-assembly method. A tight contact interface and a built-in electric field were formed between the UiO-66 and the Bi4O5Br2, which was conducive to the photo-electrons transfer from the Bi4O5Br2 to the UiO-66 and the formation of a type-II heterojunction with highly efficient charge separation. As a result, the UiO-66/Bi4O5Br2 exhibited improved photocatalytic CO2 reduction performance with a CO generation rate of 8.35 μmol h−1 g−1 without using any sacrificial agents or noble co-catalysts. This work illustrates an applicable tactic to develop potent photocatalysts for clean energy conversion.


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