Genetic transformation of commercial breeding lines of alfalfa (Medicago sativa)

1995 ◽  
Vol 42 (2) ◽  
pp. 129-140 ◽  
Author(s):  
R�jean Desgagn�s ◽  
Serge Laberge ◽  
Guy Allard ◽  
Habib Khoudi ◽  
Yves Castonguay ◽  
...  
Keyword(s):  
Genetic Transformation ◽  
Medicago Sativa ◽  
Breeding Lines ◽  
Commercial Breeding

Maternal effects and Chicken Growth

1996 ◽  
Vol 1996 ◽  
pp. 20-20
Author(s):  
J. H. Catterall ◽  
G. E. Pollott
Keyword(s):  
Body Weight ◽  
Maternal Effects ◽  
Maternal Effect ◽  
Genetic Effect ◽  
Additive Genetic Effect ◽  
Breeding Lines ◽  
Broiler Production ◽  
Female Line ◽  
Male Line ◽  
Commercial Breeding

Previous research into the genetics of broiler traits in a commercial breeding operation found a significant maternal effect on juvenile body weight, at 32 days of age, in addition to the additive genetic effect (Morris and Pollott, 1995). Since no nurture or imitative behaviour occurs between the dam and offspring in modem broiler production, any maternal effects are likely to be conferred to the offspring via the egg. This study attempts to investigate the nature of these effects.Pedigreed eggs from two separate broiler breeding lines, one experimental male line and one experimental female line, were individually weighed prior to incubation. In total 2871 eggs were weighed, 1887 eggs from the experimental female line and 984 eggs from experimental male line.

scholarly journals Improvement of the in vitro regeneration and Agrobacterium-mediated genetic transformation of Medicago sativa L.

2019 ◽  
Vol 43 (1) ◽  
pp. 96-104
Author(s):  
Farzad NOFOUZI ◽  
Muhammet Çağrı OĞUZ ◽  
Saber Delpasand KHABBAZI ◽  
Ali ERGÜL
Keyword(s):  
Genetic Transformation ◽  
Medicago Sativa ◽  
In Vitro Regeneration ◽  
Medicago Sativa L

scholarly journals Plant regeneration from proroplasts of alfalfa (Medicago sativa) via somatic embryogenesis

2003 ◽  
Vol 60 (4) ◽  
pp. 683-689 ◽  
Author(s):  
Mariza Monteiro ◽  
Beatriz Appezzato-da-Glória ◽  
Maria José Valarini ◽  
Carlos Alberto de Oliveira ◽  
Maria Lucia Carneiro Vieira
Keyword(s):  
Tissue Culture ◽  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Genetic Transformation ◽  
Medicago Sativa ◽  
Cell Suspension ◽  
High Efficiency ◽  
Point Of View ◽  
Ability To Regenerate

Alfalfa is one of the most frequently studied species from the production of tissue culture-derived embryos point of view. In this study, five alfalfa cultivars were analyzed with reference to their ability to regenerate plants from protoplast cultures via somatic embryogenesis. Plant regeneration from leaf-derived protoplasts isolated from the cultivar Rangelander was achieved using a protocol defined for alfalfa cell suspension-derived embryogenesis. Because of its high efficiency, this procedure is recommended for protoplast electroporation-mediated genetic transformation of alfalfa.

scholarly journals Efficacy of Molecular Markers Jnurf13 and AcPms1 for Prediction of Genotypes at the Nuclear Ms Locus in Three Open-pollinated Populations of Onion from North America

HortScience ◽  
2017 ◽  
Vol 52 (8) ◽  
pp. 1052-1053 ◽  
Author(s):  
Michael J. Havey ◽  
Christopher von Kohn
Keyword(s):  
Molecular Markers ◽  
North America ◽  
Male Sterility ◽  
Male Fertility ◽  
Generation Time ◽  
Male Sterile ◽  
Nuclear Markers ◽  
Breeding Lines ◽  
Long Day ◽  
Commercial Breeding

Seed of hybrid onion (Allium cepa L.) is produced using cytoplasmic male sterility (CMS). For the most widely used source of onion CMS, male sterility is conditioned by the interaction of male sterile (S) cytoplasm and the homozygous recessive genotype at the nuclear male fertility locus Ms. Because of the biennial generation time of onion, classical crossing and segregation analyses take years to establish cytoplasms and genotypes at Ms. Numerous molecular markers have been developed to distinguish onion cytoplasms and estimate genotypes at Ms. Two nuclear markers (jnurf13 and AcPms1) have been reported to cosegregate with Ms and correctly predict genotypes in commercial breeding lines and diverse onion germplasm; however, these markers were less predictive for open-pollinated (OP) populations from India. We evaluated the efficacy of jnurf13 and AcPms1 to correctly classify genotypes at Ms using 144 random plants from three OP populations of long-day onion from North America. No recombination events were detected between AcPms1 and the Ms locus and three events occurred between jnurf13 and Ms. Our results support either marker as a useful tool to predict genotypes at Ms in North American populations of onion, with AcPms1 being the better of the two.

scholarly journals Genetic Transformation and Analysis of Rice OsAPx2 Gene in Medicago sativa

PLoS ONE ◽  
2012 ◽  
Vol 7 (7) ◽  
pp. e41233 ◽  
Author(s):  
Qingjie Guan ◽  
Tetsuo Takano ◽  
Shenkui Liu
Keyword(s):  
Genetic Transformation ◽  
Medicago Sativa

scholarly journals Using markers to reduce the variation in the genomic composition in marker-assisted backcrossing

2005 ◽  
Vol 85 (2) ◽  
pp. 151-157 ◽  
Author(s):  
BERTRAND SERVIN
Keyword(s):  
Genetic Background ◽  
Genetic Composition ◽  
Major Objective ◽  
Breeding Lines ◽  
Commercial Breeding ◽  
Rate Of Recovery ◽  
Ideal Genotype ◽  
Marker Assisted Backcrossing ◽  
Number Of Individuals ◽  
Recipient Parent

Marker-assisted introgression or backcrossing is a widely used method to improve commercial breeding lines or study the effects of genes in a homogeneous genetic background. In this context, the recovery of the recipient parent genome is a major objective of backcrossing. Selection on markers has been shown to be very useful to accelerate the rate of recovery of the recipient parent genome in backcrossing. In this study we show how much information markers give on the true genetic composition of individuals by deriving the variance and estimating the distribution of the genetic composition of individuals sharing a known genotype at markers. These calculations enable predictions of the number of individuals carrying an ideal genotype at markers that must be produced to fulfil background selection objectives.

scholarly journals Genetic Diversity among Selected Medicago sativa Cultivars Using Inter-Retrotransposon-Amplified Polymorphism, Chloroplast DNA Barcodes and Morpho-Agronomic Trait Analyses

Plants ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 995
Author(s):  
Abdelfattah Badr ◽  
Nahla El-Sherif ◽  
Sara Aly ◽  
Shafik D. Ibrahim ◽  
Mohamed Ibrahim
Keyword(s):  
Genetic Diversity ◽  
Medicago Sativa ◽  
Chloroplast Dna ◽  
Genetic Relatedness ◽  
Agronomic Traits ◽  
Principal Component ◽  
Dna Barcodes ◽  
Breeding Lines ◽  
Molecular Approaches ◽  
High Forage

Alfalfa (Medicago sativa L.) is a major forage crop of family Fabaceae and is frequently cultivated in Egypt. The present study is concerned with the genetic discrimination of fifteen alfalfa cultivars from three different countries (Egypt, Australia, and USA) using two molecular approaches: inter-retrotransposon-amplified polymorphism (IRAP) markers and two chloroplast DNA barcodes matK and the trnH in addition to the analysis of fifteen morpho-agronomic traits. The genetic relatedness, based on analysis of IRAP marker polymorphism and produced using eleven primers by clustering via principal component analysis (PCA) and multivariate heatmap biostatistical methods differentiated the two Egyptian cultivars EGY1-Ismailia1 and EGY2-Nubaria1 from the three Australian and seven American cultivars, with some distinction of the cv. USA6-SW9720 and cv. AUS4-SuperFast. The results were also supported by the sequence analysis of the matK and the trnH genes on the genetic relatedness between eight cultivars. Moreover, it might be suggested that breeding lines from M. sativa cultivars may provide novel insights and a better understanding of the domestication of M. sativa genetic diversity. The classification of the eight cultivars, as revealed by morpho-agronomic traits, confirmed the close genetic relationship between the two Egyptian cultivars and indicated some resemblance between them and the AUS2-Siri Nafa, whereas the two American cultivars, USA1-Super supreme and USA4-Cuf101, were clearly isolated from a cluster of other three cultivars USA7-SW9628, USA8-Magna901, and USA9-Perfect. The results are useful sources of genetic information for future breeding programs in crop development and open new possibilities of producing M. sativa lines harboring high forage quality, productivity, and resistance to biotic and abiotic stresses.

Maternal effects and Chicken Growth

1996 ◽  
Vol 1996 ◽  
pp. 20-20
Author(s):  
J. H. Catterall ◽  
G. E. Pollott
Keyword(s):  
Body Weight ◽  
Maternal Effects ◽  
Maternal Effect ◽  
Genetic Effect ◽  
Additive Genetic Effect ◽  
Breeding Lines ◽  
Broiler Production ◽  
Female Line ◽  
Male Line ◽  
Commercial Breeding

Previous research into the genetics of broiler traits in a commercial breeding operation found a significant maternal effect on juvenile body weight, at 32 days of age, in addition to the additive genetic effect (Morris and Pollott, 1995). Since no nurture or imitative behaviour occurs between the dam and offspring in modem broiler production, any maternal effects are likely to be conferred to the offspring via the egg. This study attempts to investigate the nature of these effects.Pedigreed eggs from two separate broiler breeding lines, one experimental male line and one experimental female line, were individually weighed prior to incubation. In total 2871 eggs were weighed, 1887 eggs from the experimental female line and 984 eggs from experimental male line.

scholarly journals 034 INCORPORATION OF USEFUL TRAITS FROM NATIVE ALMOND SPECIES INTO CULTIVATED ALMOND VARIETIES

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 432e-432
Author(s):  
Dale E. Kester ◽  
Thomas M. Gradziel
Keyword(s):  
Yield Potential ◽  
Gene Introgression ◽  
Early Maturity ◽  
Breeding Lines ◽  
The Third ◽  
Hard Shell ◽  
Commercial Breeding ◽  
Third Line ◽  
Selection Of ◽  
Prunus Webbii

Approximately twenty native almond species have been described. Representative germplasm from seven of these are present in UC collections and have been used in crossing. Three specific breeding lines utilizing these species are described. One (1980 series) involved increasing yield potential through selection of high blossom density following gene introgression from Prunus fenzliana. A second involved incorporation of self-fertility, late bloom, smaller tree size, early maturity, high blossom density, and desirable nut characters from Prunus webbii into commercial breeding lines. A self-fertile selection resembling `Nonpareil' has been obtained from this material. The third line involves transmission of a unique thin, netted-surfaced, hard-shell phenotype from Prunus argentea.

Sign in / Sign up

Export Citation Format

Share Document