Mass Production of Biocontrol Agents of Insect Pests

2017 ◽  
pp. 451-465 ◽  
Author(s):  
Pradyumn Kumar ◽  
Jaswinder Kaur ◽  
J. C. Sekhar ◽  
Soujanya P. Lakshmi ◽  
S. B. Suby
Keyword(s):  
Mass Production ◽  
Insect Pests ◽  
Biocontrol Agents

scholarly journals A bioassay technique to assess the molluscicidal effects of microbes

2009 ◽  
Vol 62 ◽  
pp. 7-11 ◽  
Author(s):  
S.A. Howlett ◽  
G. Burch ◽  
U. Sarathchandra ◽  
N.L. Bell
Keyword(s):  
Insect Pests ◽  
Biocontrol Agents ◽  
Bacterial Strains ◽  
Pastoral Systems ◽  
Significant Damage ◽  
Oat Bran ◽  
Bioassay Technique ◽  
Pathogenic Microbes ◽  
Micro Organisms ◽  
Retail Products

Terrestrial slugs (Order Stylommatophora) can cause significant damage in cropping and pastoral systems Pathogenic microbes have long been shown to have potential as biocontrol agents for insect pests and a number of retail products are available but there is no equivalent product for molluscs A research program is underway to investigate the potential of micro organisms as slug biocontrol agents and this paper describes a bioassay methodology that can be used to assess the molluscicidal properties of bacterial isolates Its feasibility has been demonstrated in laboratory tests against pest slugs of the genus Deroceras Fourteen bacterial strains were screened by feeding them to the slugs in a mix based on oat bran a food that is highly palatable to these molluscs Mortality was assessed 3 and 4 days after introduction of the treated food Two of the bacterial strains tested resulted in significant mortality killing 100 slugs within 4 days

scholarly journals Lecanicillium spp. for the Management of Aphids, Whiteflies, Thrips, Scales and Mealy Bugs: Review

2020 ◽  
Author(s):  
Sajjalavarahalli Gangireddy Eswara Reddy
Keyword(s):  
Mass Production ◽  
Insect Pests ◽  
Control Agent ◽  
Natural Occurrence ◽  
Optimum Temperature ◽  
Development Of Resistance ◽  
Factors Influencing ◽  
Production Safety ◽  
Future Work ◽  
Chemical Pesticides

Lecanicillium spp. are potential microbial bio-control agent mainly used for the management of sucking insect pests such as aphids, whiteflies, scales, mealy bugs etc. and gaining much importance at present for management of pests. Due to indiscriminate use of chemical pesticides which results in development of resistance, resurgence, outbreak of pests and residue problem, the farmers/growers are forced to use bio-pesticides for sustainable agriculture. Lecanicillium spp. is promising biocontrol agent against sucking insect pests and can be used as one of the components in integrated pest management (IPM). However, optimum temperature and relative humidity are the major environmental factors, for the performance of Lecanicillium spp. under protected/field conditions. The present review is mainly focused on nomenclature of Lecanicillium spp., mode of infection, natural occurrence, influence of temperature and humidity on the growth, factors influencing the efficacy, virulence/pathogenicity to target pests, substrates used for mass production, safety to non-target organisms, compatibility with agrochemicals and commercially available products. This review is mainly useful for the researchers/students to plan their future work on Lecanicillium spp.

Optimal mass production technology for sporulation of Verticillium lecanii and Trichoderma harzianum

2015 ◽  
Vol 4 (1) ◽  
pp. 296-302
Author(s):  
Saba Hasan
Keyword(s):  
Production Technology ◽  
Trichoderma Harzianum ◽  
Mass Production ◽  
Fungal Species ◽  
Biocontrol Agents ◽  
Verticillium Lecanii ◽  
Complete Medium ◽  
Liquid Media ◽  
Solid Substrates ◽  
Viability Percentage

The present study aims at optimization of a suitable mass production technology for growth and sporulation of the biocontrol agents Verticillium lecanii and Trichoderma harzianum. Combinations of various natural solid substrates (Rice, Maize, Arhar, Defatted Soybean, Gram) and laboratory media (SMYA, Complete media, SDA, Czapek’s Dox media ) were evaluated for growth and sporulation of V. lecanii and T. harzianum. Data on CFU, concentration of propagules, dry mycelial weight, conidia production and conidia yield were analyzed. It was observed that among the substrate types tested, the fungal strains grew better and produced high quantity of spores in Rice as growth medium after 21 days of incubation followed by Maize and Arhar. The best liquid media which supported maximum conidial production was SMYA and Complete medium. Also, rate of germination of spores as well as their viability at different aeration rates was tested. It was observed that maximum length of germ tube in minimum duration was observed in Rice as substrate. The viability percentage of the biocontrol agents produced at various aeration rates and agitation speeds showed that change in aeration and agitation did not significantly affect spore viability. Results demonstrated that the fungal species could be rapidly produced with a high conidial yield on natural solid substrates as compared to liquid media by fermentation technology.

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