Isolation, Mass Production and Application of Entomopathogenic Fungi for Insect Pests Control

2019 ◽  
pp. 231-251
Author(s):  
Mohamed Abdel-Raheem
Keyword(s):  
Entomopathogenic Fungi ◽  
Mass Production ◽  
Insect Pests

Evaluation of the growth and sporulation of different entomopathogenic fungi in different liquid and solid media at varied concentrations

2017 ◽  
Vol 6 (8) ◽  
pp. 5459
Author(s):  
Chandra Teja K. ◽  
Rahman S. J.
Keyword(s):  
Entomopathogenic Fungi ◽  
Large Scale ◽  
Insect Pests ◽  
Culture Media ◽  
Virulent Strain ◽  
Carbon Sources ◽  
Nitrogen Sources ◽  
Maximum Growth ◽  
Nutritional Requirements ◽  
Virulent Strains

Entomopathogenic fungi like Beauveria bassiana, Metarhizium anisopliae and Lecanicillium lecanii are used in biological control of agricultural insect pests. Their specific mode of action makes them an effective alternative to the chemical Insecticides. Virulent strains of Entomopathogenic fungi are effectively formulated and used as bio-insecticides world-wide. Amenable and economical multiplication of a virulent strain in a large scale is important for them to be useful in the field. Culture media plays a major role in the large-scale multiplication of virulent strains of Entomopathogens. Different substrates and media components are being used for this purpose. Yet, each strain differs in its nutritional requirements for the maximum growth and hence it is necessary to standardize the right components and their optimum concentrations in the culture media for a given strain of Entomopathogen. In the current study, three different nitrogen sources and two different carbon sources were tried to standardize the mass multiplication media for seven test isolates of Entomopathogenic fungi. A study was also conducted to determine the ideal grain media for the optimum conidial yields of the test isolates. Yeast extract was found to be the best Nitrogen source for the isolates. The isolates tested, differed in their nutritional requirements and showed variation in the best nitrogen and carbon sources necessary for their growth. Variation was also found in the optimum concentration of both the ingredients for the growth and sporulation of the isolates. In the solid-state fermentation study, rice was found to be the best grain for the growth of most of the fungi followed by barley. The significance of such a study in the development of an effective Myco-insecticide is vital and can be successfully employed in agriculture is discussed.

scholarly journals Biosynthesis of Silver Nanoparticles Mediated by Entomopathogenic Fungi: Antimicrobial Resistance, Nanopesticides, and Toxicity

Antibiotics ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 852
Author(s):  
Tárcio S. Santos ◽  
Tarcisio M. Silva ◽  
Juliana C. Cardoso ◽  
Ricardo L. C. de Albuquerque-Júnior ◽  
Aleksandra Zielinska ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Filamentous Fungi ◽  
Entomopathogenic Fungi ◽  
Antimicrobial Agents ◽  
Insect Pests ◽  
Insect Control ◽  
Biological Synthesis ◽  
Biological Methods ◽  
Potential Use

Silver nanoparticles are widely used in the biomedical and agri-food fields due to their versatility. The use of biological methods for the synthesis of silver nanoparticles has increased considerably due to their feasibility and high biocompatibility. In general, microorganisms have been widely explored for the production of silver nanoparticles for several applications. The objective of this work was to evaluate the use of entomopathogenic fungi for the biological synthesis of silver nanoparticles, in comparison to the use of other filamentous fungi, and the possibility of using these nanoparticles as antimicrobial agents and for the control of insect pests. In addition, the in vitro methods commonly used to assess the toxicity of these materials are discussed. Several species of filamentous fungi are known to have the ability to form silver nanoparticles, but few studies have been conducted on the potential of entomopathogenic fungi to produce these materials. The investigation of the toxicity of silver nanoparticles is usually carried out in vitro through cytotoxicity/genotoxicity analyses, using well-established methodologies, such as MTT and comet assays, respectively. The use of silver nanoparticles obtained through entomopathogenic fungi against insects is mainly focused on mosquitoes that transmit diseases to humans, with satisfactory results regarding mortality estimates. Entomopathogenic fungi can be employed in the synthesis of silver nanoparticles for potential use in insect control, but there is a need to expand studies on toxicity so to enable their use also in insect control in agriculture.

Isolation and Selection of Entomopathogenic Fungi from Soil Samples and Evaluation of Fungal Virulence against Insect Pests

10.3791/62882 ◽  
2021 ◽  
Author(s):  
Yao-Chia Liu ◽  
Nian-Tong Ni ◽  
Ju-Chun Chang ◽  
Yi-Hsuan Li ◽  
Mi Rong Lee ◽  
...  
Keyword(s):  
Entomopathogenic Fungi ◽  
Insect Pests ◽  
Soil Samples ◽  
Fungal Virulence ◽  
Selection Of

scholarly journals Mycoviral Population Dynamics in Spanish Isolates of the Entomopathogenic Fungus Beauveria bassiana

Viruses ◽  
2018 ◽  
Vol 10 (12) ◽  
pp. 665 ◽  
Author(s):  
Charalampos Filippou ◽  
Inmaculada Garrido-Jurado ◽  
Nicolai Meyling ◽  
Enrique Quesada-Moraga ◽  
Robert Coutts ◽  
...  
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Insect Pests ◽  
Fruit Fly ◽  
Population Diversity ◽  
Viral Genomes ◽  
Large Panels ◽  
High Virulence ◽  
Fungus Beauveria Bassiana ◽  
Rna Polymerase Gene

The use of mycoviruses to manipulate the virulence of entomopathogenic fungi employed as biocontrol agents may lead to the development of novel methods to control attacks by insect pests. Such approaches are urgently required, as existing agrochemicals are being withdrawn from the market due to environmental and health concerns. The aim of this work is to investigate the presence and diversity of mycoviruses in large panels of entomopathogenic fungi, mostly from Spain and Denmark. In total, 151 isolates belonging to the genera Beauveria, Metarhizium, Lecanicillium, Purpureocillium, Isaria, and Paecilomyces were screened for the presence of dsRNA elements and 12 Spanish B. bassiana isolates were found to harbor mycoviruses. All identified mycoviruses belong to three previously characterised species, the officially recognised Beauveria bassiana victorivirus 1 (BbVV-1) and the proposed Beauveria bassiana partitivirus 2 (BbPV-2) and Beauveria bassiana polymycovirus 1 (BbPmV-1); individual B. bassiana isolates may harbor up to three of these mycoviruses. Notably, these mycovirus species are under distinct selection pressures, while recombination of viral genomes increases population diversity. Phylogenetic analysis of the RNA-dependent RNA polymerase gene sequences revealed that the current population structure in Spain is potentially a result of both vertical and horizontal mycovirus transmission. Finally, pathogenicity experiments using the Mediterranean fruit fly Ceratitis capitata showed no direct correlation between the presence of any particular mycovirus and the virulence of the B. bassiana isolates, but illustrated potentially interesting isolates that exhibit relatively high virulence, which will be used in more detailed virulence experimentation in the future.

scholarly journals Entomopathogenic fungi in Portuguese vineyards soils: suggesting a ‘Galleria-Tenebrio-bait method’ as bait-insects Galleria and Tenebrio significantly underestimate the respective recoveries of Metarhizium (robertsii) and Beauveria (bassiana)

MycoKeys ◽  
2018 ◽  
Vol 38 ◽  
pp. 1-23 ◽  
Author(s):  
Lav Sharma ◽  
Irene Oliveira ◽  
Laura Torres ◽  
Guilhermina Marques
Keyword(s):  
Functional Diversity ◽  
Entomopathogenic Fungi ◽  
Insect Pests ◽  
Natural Habitat ◽  
Habitat Type ◽  
Principal Component ◽  
Metarhizium Robertsii ◽  
Bait Method ◽  
Ecological Profiles ◽  
Soil Habitat

Entomopathogenic fungi (EPF) are the natural enemies of insect-pests. However, EPF recoveries can be influenced by the soil habitat-type(s) incorporated and/or the bait-insect(s) used. Galleriamellonella (GM) as bait-insect, i.e. ‘Galleria-bait’, is arguably the most common methodology, which is sometimes used solely, to isolate EPF from soils. Insect baiting using Tenebriomolitor (TM) has also been employed occasionally. Here 183 soils were used to estimate the functional diversity of EPF in Portuguese Douro vineyards (cultivated habitat) and adjacent hedgerows (semi-natural habitat), using the TM bait method. Moreover, to study the effect of insect baiting on EPF recovery, 81 of these 183 soil samples were also tested for EPF occurrences using the GM bait method. Twelve species were found in 44.26% ± 3.67% of the total of 183 soils. Clonostachysroseaf.rosea was found in maximum soils (30.05% ± 3.38%), followed by Beauveriabassiana (12.57% ± 2.37%), Purpureocilliumlilacinum (9.29% ± 2.14%) and Metarhiziumrobertsii (6.01% ± 1.75%). Beauveriapseudobassiana (P < 0.001), C.roseaf.rosea (P = 0.006) and Cordycepscicadae (P=0.023) were isolated significantly more from hedgerows, highlighting their sensitivities towards agricultural disturbances. Beauveriabassiana (P = 0.038) and M.robertsii (P = 0.003) were isolated significantly more using GM and TM, respectively. Principal component analysis revealed that M.robertsii was associated both with TM baiting and cultivated habitats, however, B.bassiana was slightly linked with GM baiting only. Ecological profiles of B.bassiana and P.lilacinum were quite similar while M.robertsii and C.roseaf.rosea were relatively distant and distinct. To us, this is the first report on (a) C.cicadae isolation from Mediterranean soils, (b) Purpureocilliumlavendulum as an EPF worldwide; and (c) significant recoveries of M.robertsii using TM over GM. Overall, a ‘Galleria-Tenebrio-bait method’ is advocated to study the functional diversity of EPF in agroecosystems.

BEAUVERIA BASSIANAAND OTHER ENTOMOPATHOGENIC FUNGI IN THE MANAGEMENT OF INSECT PESTS

2010 ◽  
pp. 239-276 ◽  
Author(s):  
G G Khachatourians ◽  
E P Valencia ◽  
G S Miranpuri
Keyword(s):  
Entomopathogenic Fungi ◽  
Insect Pests

Mass multiplication of Metarhizium anisopliae on different media

2020 ◽  
Vol 5 (01) ◽  
pp. 138-144
Author(s):  
Ankush Taliyan ◽  
Ajay Kumar ◽  
Rajendra Singh ◽  
Rohit Rana ◽  
Shekhar Rana
Keyword(s):  
Pest Management ◽  
Entomopathogenic Fungi ◽  
Mass Production ◽  
Metarhizium Anisopliae ◽  
Fungal Species ◽  
Mass Multiplication

Entomopathogenic fungi (EPF) Metarhizium anisopliae are fungal species that is the frequently occurring and destructive pest management to the pathogenic soil and insects. The effect of different substrates for the mass production of Metarhizium anisopliae spore/ml was significantly higher recorded The results revealed that all the treatments were significantly producing spore per ml and thus increasing the yield significantly as compared to other substrates. The results revealed that all the treatments were significantly higher effective in producing spore/ml as compared to other substrates overall finding showed that substrate tested, for Metarhizium anisopliae spore/ml production was significantly higher recorded 240.53 and 195.26 spore/ml were recorded on substrate irrespective of the temperature.

Occurrence of entomopathogenic fungi on insect pests of stored wheat and maize in Central and South Anatolia in Turkey

2016 ◽  
Vol 40 (3) ◽  
Author(s):  
Mehmet Kubilay ER ◽  
Hasan TUNAZ ◽  
Ceyda ÜCÜK ◽  
Cebrail BARIŞ ◽  
Ali Arda IŞIKBER
Keyword(s):  
Entomopathogenic Fungi ◽  
Insect Pests ◽  
Stored Wheat
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