Lignocellulase Formation, Regulation, and Secretion Mechanisms in Hypocrea jecorina (Trichoderma reesei) and Other Filamentous Fungi

2018 ◽  
pp. 43-59 ◽  
Author(s):  
Yi Jiang ◽  
Kuimei Liu ◽  
Wei Guo ◽  
Ruiqin Zhang ◽  
Fengxin Liu ◽  
...  
Keyword(s):  
Trichoderma Reesei ◽  
Filamentous Fungi ◽  
Hypocrea Jecorina

An accurate normalization strategy for RT-qPCR in Hypocrea jecorina (Trichoderma reesei)

2010 ◽  
Vol 145 (1) ◽  
pp. 30-37 ◽  
Author(s):  
Matthias G. Steiger ◽  
Robert L. Mach ◽  
Astrid R. Mach-Aigner
Keyword(s):  
Trichoderma Reesei ◽  
Hypocrea Jecorina ◽  
Normalization Strategy ◽  
Accurate Normalization

scholarly journals A Novel Cys2His2 Zinc Finger Homolog of AZF1 Modulates Holocellulase Expression in Trichoderma reesei

mSystems ◽  
2019 ◽  
Vol 4 (4) ◽  
Author(s):  
Amanda Cristina Campos Antonieto ◽  
Karoline Maria Vieira Nogueira ◽  
Renato Graciano de Paula ◽  
Luísa Czamanski Nora ◽  
Murilo Henrique Anzolini Cassiano ◽  
...  
Keyword(s):  
Trichoderma Reesei ◽  
Filamentous Fungi ◽  
Plant Cell Wall ◽  
Plant Biomass ◽  
Regulatory Elements ◽  
Biomass Degradation ◽  
Control Of Gene Expression ◽  
Content Type ◽  
Encoding Genes

ABSTRACT Filamentous fungi are remarkable producers of enzymes dedicated to the degradation of sugar polymers found in the plant cell wall. Here, we integrated transcriptomic data to identify novel transcription factors (TFs) related to the control of gene expression of lignocellulosic hydrolases in Trichoderma reesei and Aspergillus nidulans. Using various sets of differentially expressed genes, we identified some putative cis-regulatory elements that were related to known binding sites for Saccharomyces cerevisiae TFs. Comparative genomics allowed the identification of six transcriptional factors in filamentous fungi that have corresponding S. cerevisiae homologs. Additionally, a knockout strain of T. reesei lacking one of these TFs (S. cerevisiae AZF1 homolog) displayed strong reductions in the levels of expression of several cellulase-encoding genes in response to both Avicel and sugarcane bagasse, revealing a new player in the complex regulatory network operating in filamentous fungi during plant biomass degradation. Finally, RNA sequencing (RNA-seq) analysis showed the scope of the AZF1 homologue in regulating a number of processes in T. reesei, and chromatin immunoprecipitation-quantitative PCR (ChIP-qPCR) provided evidence for the direct interaction of this TF in the promoter regions of cel7a, cel45a, and swo. Therefore, we identified here a novel TF which plays a positive effect in the expression of cellulase-encoding genes in T. reesei. IMPORTANCE In this work, we used a systems biology approach to map new regulatory interactions in Trichoderma reesei controlling the expression of genes encoding cellulase and hemicellulase. By integrating transcriptomics related to complex biomass degradation, we were able to identify a novel transcriptional regulator which is able to activate the expression of these genes in response to two different cellulose sources. In vivo experimental validation confirmed the role of this new regulator in several other processes related to carbon source utilization and nutrient transport. Therefore, this work revealed novel forms of regulatory interaction in this model system for plant biomass deconstruction and also represented a new approach that could be easy applied to other organisms.

scholarly journals ACEI of Trichoderma reesei Is a Repressor of Cellulase and Xylanase Expression

2003 ◽  
Vol 69 (1) ◽  
pp. 56-65 ◽  
Author(s):  
Nina Aro ◽  
Marja Ilmén ◽  
Anu Saloheimo ◽  
Merja Penttilä
Keyword(s):  
Trichoderma Reesei ◽  
Culture Media ◽  
Carbon Sources ◽  
Cellulase Production ◽  
The Novel ◽  
Hypocrea Jecorina ◽  
Gene Encoding ◽  
Gene Coding ◽  
Better Than

ABSTRACT We characterized the effect of deletion of the Trichoderma reesei (Hypocrea jecorina) ace1 gene encoding the novel cellulase regulator ACEI that was isolated based on its ability to bind to and activate in vivo in Saccharomyces cerevisiae the promoter of the main cellulase gene, cbh1. Deletion of ace1 resulted in an increase in the expression of all the main cellulase genes and two xylanase genes in sophorose- and cellulose-induced cultures, indicating that ACEI acts as a repressor of cellulase and xylanase expression. Growth of the strain with a deletion of the ace1 gene on different carbon sources was analyzed. On cellulose-based medium, on which cellulases are needed for growth, the Δace1 strain grew better than the host strain due to the increased cellulase production. On culture media containing sorbitol as the sole carbon source, the growth of the strain with a deletion of the ace1 gene was severely impaired, suggesting that ACEI regulates expression of other genes in addition to cellulase and xylanase genes. A strain with a deletion of the ace1 gene and with a deletion of the ace2 gene coding for the cellulase and xylanase activator ACEII expressed cellulases and xylanases similar to the Δace1 strain, indicating that yet another activator regulating cellulase and xylanase promoters was present.

scholarly journals Light-dependent roles of the G-protein α subunit GNA1 of Hypocrea jecorina (anamorph Trichoderma reesei)

BMC Biology ◽  
2009 ◽  
Vol 7 (1) ◽  
pp. 58 ◽  
Author(s):  
Christian Seibel ◽  
Gabriela Gremel ◽  
Roberto do Nascimento Silva ◽  
André Schuster ◽  
Christian P Kubicek ◽  
...  
Keyword(s):  
G Protein ◽  
Trichoderma Reesei ◽  
Hypocrea Jecorina ◽  
Α Subunit ◽  
G Protein Α Subunit

scholarly journals Relevance of the light signaling machinery for cellulase expression in trichoderma reesei (hypocrea jecorina)

2010 ◽  
Vol 3 (1) ◽  
Author(s):  
Miklós Gyalai-Korpos ◽  
Gáspár Nagy ◽  
Zoltán Mareczky ◽  
André Schuster ◽  
Kati Réczey ◽  
...  
Keyword(s):  
Trichoderma Reesei ◽  
Light Signaling ◽  
Hypocrea Jecorina ◽  
Cellulase Expression

scholarly journals Molecular evidence that the asexual industrial fungus Trichoderma reesei is a clonal derivative of the ascomycete Hypocrea jecorina.

1996 ◽  
Vol 93 (15) ◽  
pp. 7755-7760 ◽  
Author(s):  
K. Kuhls ◽  
E. Lieckfeldt ◽  
G. J. Samuels ◽  
W. Kovacs ◽  
W. Meyer ◽  
...  
Keyword(s):  
Trichoderma Reesei ◽  
Molecular Evidence ◽  
Hypocrea Jecorina
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