Three-Dimensional Cell Culture and Tissue Restoration of Neural Stem Cells Under Microgravity

2019 ◽  
pp. 235-279
Author(s):  
Jin Han ◽  
Yi Cui ◽  
Bai Xu ◽  
Weiwei Xue ◽  
Sumei Liu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Neural Stem Cells ◽  
Three Dimensional ◽  
Tissue Restoration ◽  
Dimensional Cell

A novel three-dimensional cell culture platform for proliferating stem cells for cell therapy

Cytotherapy ◽  
2013 ◽  
Vol 15 (4) ◽  
pp. S44
Author(s):  
H. Zhang
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Cell Therapy ◽  
Three Dimensional ◽  
Dimensional Cell

Effective Gene Delivery to Mesenchymal Stem Cells Based on the Reverse Transfection and Three-Dimensional Cell Culture System

2011 ◽  
Vol 28 (7) ◽  
pp. 1577-1590 ◽  
Author(s):  
Cai-Xia He ◽  
Ni Li ◽  
Yu-Lan Hu ◽  
Xiu-Mei Zhu ◽  
Hai-Jie Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Mesenchymal Stem Cells ◽  
Gene Delivery ◽  
Culture System ◽  
Three Dimensional ◽  
Cell Culture System ◽  
Reverse Transfection ◽  
Dimensional Cell

scholarly journals Growing, Tracking, and Directing Bone Marrow Derived Stem Cells From Two-Dimensional and Three-Dimensional Cell Culture Microenvironments

2019 ◽  
Author(s):  
Tiffany Miller
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Bone Marrow ◽  
Stem Cell ◽  
Three Dimensional ◽  
Inflammatory Biomarkers ◽  
Two Dimensional ◽  
Cell Culturing ◽  
Dimensional Cell ◽  
Stimulation Of

<p>Bone marrow derived stem cells express biomarkers capable of facilitating adhesion to the cell culturing microenvironment, thereby, influencing their proliferation, migration, and differentiation. In particular, biological biomarkers of mesenchymal stem cells include, but are not limited to, CD14-, CD19-, CD34-, CD45-, CD29, CD44, CD73+, CD90+, CD105+, CD106, CD166, Stro-1, and HLADR. The relationship between the stem cell biology and the materials and methods forming a cell culturing microenvironment serves as a critical aspect in the successful adhesion and growth within two-dimensional cell culture microenvironments such as polystyrene, laminin, fibronectin, or poly-L-lysine and within three-dimensional cell culture microenvironments such as hydrogel, ceramic, collagen, polymer based nanofibers, agitation, forced floating, and hang drop systems. Further, electrical stimulation of the stem cells may be implemented during the cell culturing process to measure stem cell growth and to determine stem cell viability. In addition, electrical stimulation of implanted stem cells may facilitate tracking by measuring stem cell migration distance and travel area. Although many biochemical and inflammatory biomarkers are expressed based on severity in stroke including, but not limited to, Interluken-6 (IL-6), tumor necrosis factor-alpha (TNF-α), and glutamate (Glu), current methodologies of stem cell directing lack localization and biological effector specificity. Biological effector bound magnetic particle stem cells may serve as a potential treatment method in ischemic stroke. In particular, a stem cell biomarker may be configured to communicate with inflammatory biomarkers, thus, more efficiently delivering the stem cells to site specific areas having the most severely affected <i>in-vivo</i> biochemical microenvironments.</p>

scholarly journals Growing, Tracking, and Directing Bone Marrow Derived Stem Cells From Two-Dimensional and Three-Dimensional Cell Culture Microenvironments

2019 ◽  
Author(s):  
Tiffany Miller
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Bone Marrow ◽  
Stem Cell ◽  
Three Dimensional ◽  
Inflammatory Biomarkers ◽  
Two Dimensional ◽  
Cell Culturing ◽  
Dimensional Cell ◽  
Stimulation Of

<p>Bone marrow derived stem cells express biomarkers capable of facilitating adhesion to the cell culturing microenvironment, thereby, influencing their proliferation, migration, and differentiation. In particular, biological biomarkers of mesenchymal stem cells include, but are not limited to, CD14-, CD19-, CD34-, CD45-, CD29, CD44, CD73+, CD90+, CD105+, CD106, CD166, Stro-1, and HLADR. The relationship between the stem cell biology and the materials and methods forming a cell culturing microenvironment serves as a critical aspect in the successful adhesion and growth within two-dimensional cell culture microenvironments such as polystyrene, laminin, fibronectin, or poly-L-lysine and within three-dimensional cell culture microenvironments such as hydrogel, ceramic, collagen, polymer based nanofibers, agitation, forced floating, and hang drop systems. Further, electrical stimulation of the stem cells may be implemented during the cell culturing process to measure stem cell growth and to determine stem cell viability. In addition, electrical stimulation of implanted stem cells may facilitate tracking by measuring stem cell migration distance and travel area. Although many biochemical and inflammatory biomarkers are expressed based on severity in stroke including, but not limited to, Interluken-6 (IL-6), tumor necrosis factor-alpha (TNF-α), and glutamate (Glu), current methodologies of stem cell directing lack localization and biological effector specificity. Biological effector bound magnetic particle stem cells may serve as a potential treatment method in ischemic stroke. In particular, a stem cell biomarker may be configured to communicate with inflammatory biomarkers, thus, more efficiently delivering the stem cells to site specific areas having the most severely affected <i>in-vivo</i> biochemical microenvironments.</p>

scholarly journals Squaramide-Based Supramolecular Materials for Three-Dimensional Cell Culture of Human Induced Pluripotent Stem Cells and Their Derivatives

2018 ◽  
Vol 19 (4) ◽  
pp. 1091-1099 ◽  
Author(s):  
Ciqing Tong ◽  
Tingxian Liu ◽  
Victorio Saez Talens ◽  
Willem E. M. Noteborn ◽  
Thomas H. Sharp ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Three Dimensional ◽  
Supramolecular Materials ◽  
Induced Pluripotent ◽  
Dimensional Cell

scholarly journals Embryonic stem cells in scaffold-free three-dimensional cell culture: osteogenic differentiation and bone generation

2011 ◽  
Vol 7 (1) ◽  
Author(s):  
Jörg Handschel ◽  
Christian Naujoks ◽  
Rita Depprich ◽  
Lydia Lammers ◽  
Norbert Kübler ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Embryonic Stem Cells ◽  
Osteogenic Differentiation ◽  
Three Dimensional ◽  
Embryonic Stem ◽  
Dimensional Cell

scholarly journals Three-dimensional cell culture of human mesenchymal stem cells in nanofibrillar cellulose hydrogels

2017 ◽  
Vol 7 (3) ◽  
pp. 458-465 ◽  
Author(s):  
Ioannis Azoidis ◽  
Joel Metcalfe ◽  
James Reynolds ◽  
Shirley Keeton ◽  
Sema S. Hakki ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Mesenchymal Stem Cells ◽  
Three Dimensional ◽  
Human Mesenchymal Stem Cells ◽  
Nanofibrillar Cellulose ◽  
Image Position ◽  
Dimensional Cell

Abstract

The Effect of Two- and Three-Dimensional Cell Culture on the Chondrogenic Potential of Human Adipose-Derived Mesenchymal Stem Cells after Subcutaneous Transplantation with an Injectable Hydrogel

2011 ◽  
Vol 20 (10) ◽  
pp. 1575-1588 ◽  
Author(s):  
Christophe Merceron ◽  
Sophie Portron ◽  
Martial Masson ◽  
Julie Lesoeur ◽  
Borhane Hakim Fellah ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Mesenchymal Stem Cells ◽  
Three Dimensional ◽  
Injectable Hydrogel ◽  
Chondrogenic Potential ◽  
Subcutaneous Transplantation ◽  
Dimensional Cell
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