Prognosis and Survival in Gall Bladder Cancer

2021 ◽  
pp. 247-259
Author(s):  
Vinay K. Kapoor
Keyword(s):  
Bladder Cancer ◽  
Gall Bladder ◽  
Gall Bladder Cancer

Jab1-siRNA Induces Cell Growth Inhibition and Cell Cycle Arrest in Gall Bladder Cancer Cells via Targeting Jab1 Signalosome

2020 ◽  
Vol 19 (16) ◽  
pp. 2019-2033 ◽  
Author(s):  
Pratibha Pandey ◽  
Mohammad H. Siddiqui ◽  
Anu Behari ◽  
Vinay K. Kapoor ◽  
Kumudesh Mishra ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Bladder Cancer ◽  
Cell Growth ◽  
Gall Bladder ◽  
Growth Inhibition ◽  
Cell Cycle Arrest ◽  
Gall Bladder Cancer ◽  
Cell Growth Inhibition ◽  
Cycle Arrest ◽  
Apoptotic Induction

Background: The aberrant alteration in Jab1 signalosome (COP9 Signalosome Complex Subunit 5) has been proven to be associated with the progression of several carcinomas. However the specific role and mechanism of action of Jab1 signalosome in carcinogenesis of gall bladder cancer (GBC) are poorly understood. Objective: The main objective of our study was to elucidate the role and mechanism of Jab1 signalosome in gall bladder cancer by employing siRNA. Methods: Jab1 overexpression was identified in gall bladder cancer tissue sample. The role of Jab1-siRNA approach in cell growth inhibition and apoptotic induction was then examined by RT-PCR, Western Blotting, MTT, ROS, Hoechst and FITC/Annexin-V staining. Results: In the current study, we have shown that overexpression of Jab1 stimulated the proliferation of GBC cells; whereas downregulation of Jab1 by using Jab1-siRNA approach resulted incell growth inhibition and apoptotic induction. Furthermore, we found that downregulation of Jab1 induces cell cycle arrest at G1 phase and upregulated the expression of p27, p53 and Bax gene. Moreover, Jab1-siRNA induces apoptosis by enhancing ROS generation and caspase-3 activation. In addition, combined treatment with Jab1-siRNA and gemicitabine demonstrated an enhanced decline in cell proliferation which further suggested increased efficacy of gemcitabine at a very lower dose (5μM) in combination with Jab1-siRNA. Conclusion: In conclusion, our study strongly suggests that targeting Jab1 signalosome could be a promising therapeutic target for the treatment of gall bladder cancer.

Elucidation of the Chemopreventive Role of Stigmasterol Against Jab1 in Gall Bladder Carcinoma

2019 ◽  
Vol 19 (6) ◽  
pp. 826-837 ◽  
Author(s):  
Pratibha Pandey ◽  
Preeti Bajpai ◽  
Mohammad H. Siddiqui ◽  
Uzma Sayyed ◽  
Rohit Tiwari ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Bladder Cancer ◽  
Gall Bladder ◽  
Cancer Cells ◽  
Caspase 3 ◽  
Annexin V ◽  
Gall Bladder Cancer ◽  
Apoptosis Induction ◽  
Bladder Cancer Cells ◽  
Hoechst Staining

Background:Plant sterols have proven a potent anti-proliferative and apoptosis inducing agent against several carcinomas including breast and prostate cancers. Jab1 has been reported to be involved in the progression of numerous carcinomas. However, antiproliferative effects of sterols against Jab1 in gall bladder cancer have not been explored yet.Objective:In the current study, we elucidated the mechanism of action of stigmasterol regarding apoptosis induction mediated via downregulation of Jab1 protein in human gall bladder cancer cells.Methods:In our study, we performed MTT and Trypan blue assay to assess the effect of stigmasterol on cell proliferation. In addition, RT-PCR and western blotting were performed to identify the effect of stigmasterol on Jab1 and p27 expression in human gall bladder cancer cells. We further performed cell cycle, Caspase-3, Hoechst and FITC-Annexin V analysis, to confirm the apoptosis induction in stigmasterol treated human gall bladder cancer cells.Results:Our results clearly indicated that stigmasterol has up-regulated the p27 expression and down-regulated Jab1 gene. These modulations of genes might occur via mitochondrial apoptosis signaling pathway. Caspase-3 gets activated with the apoptotic induction. Increase in apoptotic cells and DNA were confirmed through annexin V staining, Hoechst staining, and cell cycle analysis.Conclusion:Thus, these results strongly suggest that stigmasterol has the potential to be considered as an anticancerous therapeutic agent against Jab1 in gall bladder cancer.

Descriptive epidemiology of gall-bladder cancer in Europe

1993 ◽  
Vol 119 (3) ◽  
pp. 165-171 ◽  
Author(s):  
Witold Zatonskí ◽  
Carlo La Vecchia ◽  
Fabio Levi ◽  
Eva Negri ◽  
Franca Lucchini
Keyword(s):  
Bladder Cancer ◽  
Gall Bladder ◽  
Gall Bladder Cancer ◽  
Descriptive Epidemiology

Role of Combination Chemotherapy with 5-Fluorouracil, Cisplatin and Paclitaxel for Advanced Gall Bladder Cancer

2014 ◽  
Vol 05 (03) ◽  
pp. 246-252 ◽  
Author(s):  
Mumtaz Ahmad Ansari ◽  
Satyendra K. Tiwary ◽  
Uday Pratap Shahi ◽  
Vijay K. Shukla
Keyword(s):  
Bladder Cancer ◽  
Gall Bladder ◽  
Combination Chemotherapy ◽  
Gall Bladder Cancer ◽  
Advanced Gall Bladder Cancer

The role of H. pylori infection in gall bladder cancer: clinicopathological study

Tumor Biology ◽  
2015 ◽  
Vol 36 (9) ◽  
pp. 7093-7098 ◽  
Author(s):  
Ehsan Hassan Hassan ◽  
Shawkat S. Gerges ◽  
Kamal A. El-Atrebi ◽  
Hala T. El-Bassyouni
Keyword(s):  
Bladder Cancer ◽  
Gall Bladder ◽  
Gall Bladder Cancer ◽  
Clinicopathological Study ◽  
H Pylori

scholarly journals Outcomes following surgical resection of 515 patients with gall bladder cancer: Indian experience

HPB ◽  
2016 ◽  
Vol 18 ◽  
pp. e52
Author(s):  
M. Goel ◽  
S. Patkar ◽  
M. Bhandare ◽  
V. Chaudhari ◽  
A. Mitra ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Gall Bladder ◽  
Surgical Resection ◽  
Gall Bladder Cancer ◽  
Indian Experience

Gall Bladder Cancer with Jaundice: The Unscaled Frontier

2018 ◽  
pp. 119-130
Author(s):  
Anu Behari ◽  
V. K. Kapoor
Keyword(s):  
Bladder Cancer ◽  
Gall Bladder ◽  
Gall Bladder Cancer
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