Transcriptional activation of 2 classes of genes during the hypersensitive reaction of tobacco leaves infiltrated with an incompatible isolate of the phytopathogenic bacterium Pseudomonas solanacearum

ABSTRACT The deduced amino acid sequences of the flagellins of Pseudomonas syringae pv. tabaci and P. syringae pv. glycinea are identical; however, their abilities to induce a hypersensitive reaction are clearly different. The reason for the difference seems to depend on the posttranslational modification of the flagellins. To investigate the role of this posttranslational modification in the interactions between plants and bacterial pathogens, we isolated genes that are potentially involved in the posttranslational modification of flagellin in P. syringae pv. glycinea (glycosylation island); then defective mutants with mutations in these genes were generated. There are three open reading frames in the glycosylation island, designated orf1, orf2, and orf3. orf1 and orf2 encode putative glycosyltransferases, and mutants with defects in these open reading frames, Δorf1 and Δorf2, secreted nonglycosylated and slightly glycosylated flagellins, respectively. Inoculation tests performed with these mutants and original nonhost tobacco leaves revealed that Δorf1 and Δorf2 could grow on tobacco leaves and caused symptom-like changes. In contrast, these mutants failed to cause symptoms on original host soybean leaves. These data indicate that putative glycosyltransferases encoded in the flagellin glycosylation island are strongly involved in recognition by plants and could be the specific determinants of compatibility between phytopathogenic bacteria and plant species.

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Antibacterial activity of sesquiterpenoids from tobacco leaves elicited by Pseudomonas solanacearum and tobacco mosaic virus.

Japanese Journal of Phytopathology ◽

10.3186/jjphytopath.49.501 ◽

1983 ◽

Vol 49 (4) ◽

pp. 501-507 ◽

Cited By ~ 6

Author(s):

Hiroshi TANAKA ◽

Reiko UEGAKI ◽

Takane FUJIMORI ◽

Kunio KATO

Keyword(s):

Antibacterial Activity ◽

Tobacco Mosaic Virus ◽

Mosaic Virus ◽

Pseudomonas Solanacearum ◽

Tobacco Leaves

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Ammonia as a necrotoxin in the hypersensitive reaction caused by bacteria in tobacco leaves

Canadian Journal of Botany ◽

10.1139/b70-023 ◽

1970 ◽

Vol 48 (1) ◽

pp. 167-171 ◽

Cited By ~ 10

Author(s):

L. Lovrekovich ◽

H. Lovrekovich ◽

R. N. Goodman

Keyword(s):

Erwinia Amylovora ◽

Hypersensitive Reaction ◽

Tissue Necrosis ◽

Ammonia Gas ◽

Tobacco Leaves

Tobacco leaves inoculated with 108 cells/ml of the incompatible bacteria Pseudomonas pisi, P. syringae, P. lachrymans, or Erwinia amylovora developed hypersensitive tissue necrosis, on the first day after inoculation. Similar tissue necrosis developed on the leaves inoculated with the compatible bacterium P. tabaci on the second day. By the time the symptoms developed ammonia gas had evolved from the inoculated leaves. Tissue necrosis caused by bacteria could be reproduced by exposing healthy tobacco leaves to ammonia gas. The amount of ammonia evolved during the development of either the hypersensitive reactions or the disease was enough to account for the formation of tissue necrosis in tobacco.

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