scholarly journals Isolation and characterization of a microspore-specific gene from tobacco

1996 ◽  
Vol 31 (2) ◽  
pp. 213-225 ◽  
Author(s):  
M. T. Oldenhof ◽  
P. F. M. de Groot ◽  
J. H. Visser ◽  
J. A. M. Schrauwen ◽  
G. J. Wullems
Keyword(s):  
Specific Gene ◽  
Isolation And Characterization

Isolation and characterization of a novelcis-acting sequences regulating root-specific gene fromDaucus carota L

2004 ◽  
Vol 49 (22) ◽  
pp. 2393-2398
Author(s):  
Yan Liu ◽  
Chang-an Guo ◽  
Haibo Ren ◽  
Fan Chen
Keyword(s):  
Specific Gene ◽  
Isolation And Characterization

Isolation and Characterization of a Novel Hair Follicle–Specific Gene, Hacl-1

1994 ◽  
Vol 102 (5) ◽  
pp. 716-720 ◽  
Author(s):  
Nam-Ho Huh ◽  
Mariko Kashiwagi ◽  
Chieko Konishi ◽  
Y.u. Hashimoto ◽  
Yohko Kohno ◽  
...  
Keyword(s):  
Hair Follicle ◽  
Specific Gene ◽  
Isolation And Characterization

Isolation and characterization of a mouse hair follicle-specific gene

1993 ◽  
Vol 6 (1) ◽  
pp. 21
Author(s):  
N. Huh ◽  
C. Konishi ◽  
M. Kashiwagi ◽  
O. Lei ◽  
Y. Kohno ◽  
...  
Keyword(s):  
Hair Follicle ◽  
Specific Gene ◽  
Isolation And Characterization

Isolation and characterization of a cellulose-growth-specific gene from Agaricus bisporus

Gene ◽  
1992 ◽  
Vol 119 (2) ◽  
pp. 183-190 ◽  
Author(s):  
S. Raguz ◽  
E. Yaguea ◽  
D.A. Wood ◽  
C.F. Thurston
Keyword(s):  
Agaricus Bisporus ◽  
Specific Gene ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of CaMF3, an anther-specific gene in Capsicum annuum L.

2012 ◽  
Vol 35 (4) ◽  
pp. 810-817 ◽  
Author(s):  
Xuefeng Hao ◽  
Changming Chen ◽  
Guoju Chen ◽  
Bihao Cao ◽  
Qinghua Chen ◽  
...  
Keyword(s):  
Capsicum Annuum ◽  
Specific Gene ◽  
Capsicum Annuum L ◽  
Isolation And Characterization

Isolation and Characterization of a Microsporocyte-Specific Gene, OsMSP, in Rice

2009 ◽  
Vol 27 (4) ◽  
pp. 469-475 ◽  
Author(s):  
Dagang Jiang ◽  
Jing Li ◽  
Ping Wu ◽  
Zhenlan Liu ◽  
Chuxiong Zhuang
Keyword(s):  
Specific Gene ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of a new cell line of retinoblastoma

2017 ◽  
Author(s):  
Fariba Ghasemi ◽  
Saeed Heidari Keshel ◽  
Mojgan Mirakhorli ◽  
Reza Roozafzoon ◽  
Mohammad Taher Rajabi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
Cultured Cells ◽  
Primary Cultures ◽  
Established Cell Line ◽  
Specific Gene ◽  
Pathology Report ◽  
New Cell Line ◽  
Isolation And Characterization

BACKGROUNDː This article describes the characterization of a new cell line (IRB-1), which was established from an Iranian child with retinoblastoma. METHODSː Using cell culture techniques, we isolated a clonal population from primary Cultures of retinoblastoma tumor samples and after several passages an established cell line, IRB-1, was obtained. The following techniques were performed: cell proliferation studies by MTT assays, scanning electron microscopy, cell surface analyses by flowcytometry, creation of neurospheres in soft agar and the expression of ABCG2, RAR-α, GFAP, and ACTB genes by real time PCR. RESULTSː Of total 6 received samples, only a single cell line was successfully isolatedfrom a group E sporadic unilateral retinoblastoma. The primarily enucleated left eye had a differentiated retinoblastoma, with a slight degree of necrosis in pathology report. The adherent cultured cells were in spindle-shaped morphology with similar appendages and pulled neurons. Doubling time is 36 ± 5 hours. After 30 days, the effect of retinoic acid on isolated cells and expression of RAR-α receptor was appeared. Chromosomal abnormalities in chromosomes 3, 9, 10, 11, 14, 16, 17, and 22 were observed. Specific gene expression of ABCG2, RAR-α, and ACTB gene expression of GFAP positive expression of these genes in the control cells were observed. This cell line passaged 132 consecutive times. Discussionː IRB-1 is a novel human adherent RB cell line, which with interesting characteristics of adherent and flocculent growth and more chromosomal imbalances different from other RB cell lines. A novel human RB cell line, IRB-1, was established from a 1-year old patient with a unilateral sporadic RB at Farabi Hospital- Tehran University Medical Sciences. The cultural characteristics of IRB-1 cell line were comparable to those of Y79 in characteristic.

scholarly journals Isolation and Characterization of Integron-Containing Bacteria without Antibiotic Selection

2004 ◽  
Vol 48 (3) ◽  
pp. 838-842 ◽  
Author(s):  
Robert S. Barlow ◽  
John M. Pemberton ◽  
Patricia M. Desmarchelier ◽  
Kari S. Gobius
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Antibiotic Resistance Genes ◽  
Meat Production ◽  
Specific Gene ◽  
Antibiotic Selection ◽  
Selective Enrichment ◽  
Specific Pcr ◽  
Isolation And Characterization

ABSTRACT The emergence of antibiotic resistance among pathogenic and commensal bacteria has become a serious problem worldwide. The use and overuse of antibiotics in a number of settings are contributing to the development of antibiotic-resistant microorganisms. The class 1 and 2 integrase genes (intI1 and intI2, respectively) were identified in mixed bacterial cultures enriched from bovine feces by growth in buffered peptone water (BPW) followed by integrase-specific PCR. Integrase-positive bacterial colonies from the enrichment cultures were then isolated by using hydrophobic grid membrane filters and integrase-specific gene probes. Bacterial clones isolated by this technique were then confirmed to carry integrons by further testing by PCR and DNA sequencing. Integron-associated antibiotic resistance genes were detected in bacteria such as Escherichia coli, Aeromonas spp., Proteus spp., Morganella morganii, Shewanella spp., and urea-positive Providencia stuartii isolates from bovine fecal samples without the use of selective enrichment media containing antibiotics. Streptomycin and trimethoprim resistance were commonly associated with integrons. The advantages conferred by this methodology are that a wide variety of integron-containing bacteria may be simultaneously cultured in BPW enrichments and culture biases due to antibiotic selection can be avoided. Rapid and efficient identification, isolation, and characterization of antibiotic resistance-associated integrons are possible by this protocol. These methods will facilitate greater understanding of the factors that contribute to the presence and transfer of integron-associated antibiotic resistance genes in bacterial isolates from red meat production animals.

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