Inhibition of flower induction on in vitro chicory root explants by hydroponic forcing pretreatment of roots

1995 ◽  
Vol 17 (3) ◽  
pp. 199-203 ◽  
Author(s):  
M. A. C. Demeulemeester ◽  
H. Vangossum ◽  
M. P. De Proft
Keyword(s):  
Flower Induction ◽  
Root Explants ◽  
Chicory Root

Induction of stem elongation on in vitro chicory root explants under unfavourable photoperiodic conditions by gibberellin A3

1995 ◽  
Vol 16 (3) ◽  
pp. 239-241 ◽  
Author(s):  
M. A. C. Demeulemeester ◽  
A. Voet ◽  
M. P. De Proft
Keyword(s):  
Stem Elongation ◽  
Root Explants ◽  
Gibberellin A3 ◽  
Chicory Root

Use of Chicory Root Explants In Vitro as a Model for the Induction of Different Stem Types

2000 ◽  
Vol 156 (3) ◽  
pp. 413-418 ◽  
Author(s):  
M.A.C. Demeulemeester ◽  
M.P. De Proft
Keyword(s):  
Root Explants ◽  
Chicory Root

A phytochrome-mediated alteration from stem to rosette growth on chicory root explants in vitro

1996 ◽  
Vol 18 (3) ◽  
pp. 207-212 ◽  
Author(s):  
M. A. C. Demeulemeester ◽  
H. Asard ◽  
M. P. De Proft
Keyword(s):  
Root Explants ◽  
Chicory Root

Stem elongation and floral initiation on in vitro chicory root explants: influence of photoperiod

1995 ◽  
Vol 16 (3) ◽  
pp. 233-238 ◽  
Author(s):  
M. A. C. Demeulemeester ◽  
A. Voet ◽  
Van de Mierop ◽  
M. P. de Proft
Keyword(s):  
Stem Elongation ◽  
Floral Initiation ◽  
Root Explants ◽  
Chicory Root

Utilization of Different Seedling Explants for in Vitro Propagation of Hibiscus syriacus

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 478e-479
Author(s):  
M.M. Jenderek ◽  
A.J. Olney
Keyword(s):  
Callus Formation ◽  
Adventitious Bud ◽  
Root Explants ◽  
Petiole Explants ◽  
Bud Formation ◽  
Hypocotyl Explants ◽  
Leaf Petiole ◽  
Adventitious Bud Formation ◽  
Hibiscus Syriacus

Hibiscus syriacus is a difficult species in micropropagation due to its endogenous contamination and recalcitrant shoot formation; therefore, studies on using explants other than shoot tip or axillary buds of growing shrubs were initiated. Three different seedling fragments (root, hypocotyl, and leaf petiole) from aseptically germinated seedlings of hibiscus (var. Aphrodite) were evaluated for adventitious bud formation, shoot and leaf development. The explants were cultured on McCown's woody plant basal salt medium supplemented with KNO3 (800 mg/L), adenine sulfate (80 mg/L) and MS vitamins containing BA or 2iP or TDZ at 0.5, 1.0, 2.2, 4.4 and 10 mM. Adventitious buds were present on all of the three different explants grown on medium containing TDZ; however, the most abundant bud formation, with many small leaves originating from callus was observed on hypocotyl explants cultured on medium with 1 mM of TDZ. Petiole explants were the most frequent to develop short shoots (≈15 mm) and one to nine leaves without callus formation, where 70% of hypocotyl and the root explants formed leaves originating from callus. Callus was induced on all explant types regardless of the level or type of cytokinin used. However, the number of shoots produced by any explant type was low, petioles cultured on 0.5 and 1mM of TDZ were the most suitable material for non-callus shoot development in H. syriacus. Hypocotyl explants proved to be an excellent source for adventitious bud formation but their ability to develop shoots needs to be investigated.

scholarly journals Natural Variation in Plant Pluripotency and Regeneration

Plants ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1261
Author(s):  
Robin Lardon ◽  
Danny Geelen
Keyword(s):  
De Novo ◽  
Shoot Organogenesis ◽  
In Vitro Regeneration ◽  
Relative Role ◽  
Root Explants ◽  
Experimental Systems ◽  
Complex Process ◽  
Insight Into ◽  
Molecular Framework

Plant regeneration is essential for survival upon wounding and is, hence, considered to be a strong natural selective trait. The capacity of plant tissues to regenerate in vitro, however, varies substantially between and within species and depends on the applied incubation conditions. Insight into the genetic factors underlying this variation may help to improve numerous biotechnological applications that exploit in vitro regeneration. Here, we review the state of the art on the molecular framework of de novo shoot organogenesis from root explants in Arabidopsis, which is a complex process controlled by multiple quantitative trait loci of various effect sizes. Two types of factors are distinguished that contribute to natural regenerative variation: master regulators that are conserved in all experimental systems (e.g., WUSCHEL and related homeobox genes) and conditional regulators whose relative role depends on the explant and the incubation settings. We further elaborate on epigenetic variation and protocol variables that likely contribute to differential explant responsivity within species and conclude that in vitro shoot organogenesis occurs at the intersection between (epi) genetics, endogenous hormone levels, and environmental influences.

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