Vigor and variation expressed by anther-derived doubled haploids of burley tobacco (Nicotiana tabacum L.). I. Comparison of sexual and doubled-haploid populations

Euphytica ◽  
1986 ◽  
Vol 35 (1) ◽  
pp. 33-40 ◽  
Author(s):  
W. R. Deaton ◽  
G. B. Collins ◽  
M. T. Nielsen
1983 ◽  
Vol 63 (2) ◽  
pp. 515-521
Author(s):  
M. J. KASPERBAUER ◽  
H. R. BURTON

The petroleum ether extractable (PEE) fraction of burley tobacco (Nicotiana tabacum L.) was studied in doubled haploid lines. The fraction contains components, such as neophytadiene, that are associated with flavor and aroma, and some that contribute to biological activity of tobacco smoke. The PEE contents of 355 haploid plants ranged from 34 to 67 mg/g of dry matter. Doubled haploids derived from haploid plants that ranked at the high and low ends of the range were grown under greenhouse and field conditions, and were sampled at harvest and after conventional air-curing. Total PEE concentrations remained different for the two groups of doubled haploids within each year and location; however, concentrations for both groups decreased during conventional air-curing, and 1979 field-grown cured leaves had less than cured leaves from the 1980 season. Neophytadiene content of field-grown, air-cured tobacco was greatly influenced by environment. Concentration was almost double in 1980 relative to 1979, and nearly tripled during curing. The normal, iso and anteiso classes of the total saturated hydrocarbons occurred in relatively the same percentages in green or cured field-grown tobacco leaves and with no apparent pattern according to the PEE groupings. Doubled haploids and controlled environments offer a means to study effects of individual environmental parameters on changes in amount and composition of the PEE fraction.Key words: Nicotiana tabacum L., burley tobacco, doubled haploids, neophytadiene, C25-C33 hydrocarbons


Author(s):  
Monika Agacka-Mołdoch ◽  
Mian Abdur Rehman Arif ◽  
Ulrike Lohwasser ◽  
Teresa Doroszewska ◽  
Ramsey S. Lewis ◽  
...  

AbstractGenetic mapping of seed germination traits has been performed with many plant species. In tobacco, however, investigations are rare. In the present study, a bi-parental mapping population consisting of 118 doubled haploid lines and derived from a cross between ‘Beinhart-1000’ and ‘Hicks’ was investigated. Four germination-related traits, total germination (TG), normal germination (NG), time to reach 50% of total germination (T50), and the area under the curve after 200 h of germination (AUC) were considered by examining seeds either untreated or after a moderate controlled deterioration (CD). Quantitative trait loci were found for all traits distributed on 11 out of the 24 linkage groups. It was demonstrated that, as in many other species, germination-related traits are very complex and under polygenic control.


1974 ◽  
Vol 54 (1) ◽  
pp. 167-174 ◽  
Author(s):  
B. D. FUQUA ◽  
J. E. LEGGETT ◽  
J. L. SIMS

Experiments were conducted to study the NO3− and Cl− interactions during uptake by burley tobacco (Nicotiana tabacum L., cult Ky 14) and the effects of their interactions on plant composition over long growth periods. Results indicated that NO3− or Cl− inhibited the absorption of the other anion by burley tobacco plants but did not influence the composition of cations. Positive linear relationships were found between NO3− and Cl− levels in solution, and the NO3− and Cl− contents in the plants in the laboratory and greenhouse studies. The inhibition of NO3− by Cl− during accumulation was also evident for tobacco plants grown under field conditions for longer periods. The interaction between NO3− and Cl− appears to be competitive during accumulation by burley tobacco. Competition between NO3− and Cl− during accumulation is proposed to be localized in the leaf cell rather than at the root–media interface.


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