Influence of plant growth regulators, basal media and carbohydrate levels on the in vitro development of Pinus ponderosa (Dougl. ex Law.) cotyledon explants

1990 ◽  
Vol 20 (1) ◽  
pp. 47-52 ◽  
Author(s):  
G. A. Tuskan ◽  
W. A. Sargent ◽  
T. Rensema ◽  
J. A. Walla
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Pinus Ponderosa ◽  
In Vitro Development ◽  
Cotyledon Explants ◽  
Basal Media ◽  
Carbohydrate Levels ◽  
Vitro Development

scholarly journals Effects of different types of sugars and plant growth regulators on kohlrabi seedling growth and development in vitro

2020 ◽  
Vol 72 (3) ◽  
pp. 349-357
Author(s):  
Tatjana Cosic ◽  
Jelena Savic ◽  
Martin Raspor ◽  
Aleksandar Cingel ◽  
Nabil Ghalawnji ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Growth And Development ◽  
Different Types ◽  
Half Strength ◽  
Plant Stem ◽  
Development In Vitro ◽  
Vitro Development

Kohlrabi (Brassica oleracea var. gongylodes), with its edible stem tuber formed at the base of the plant stem, presents a valuable source of nutrients. The potential effects of plant growth regulators (PGRs), as well as various concentrations of different sugars on the in vitro development of kohlrabi were studied. Ten-day-old kohlrabi seedlings were cultivated in vitro for 5 weeks at 18?2?C on half-strength MS media containing different concentrations of carbon source such as sucrose, fructose, glucose, xylose and mannitol, combined with or without specific plant growth regulators (N6-benzyladenine (BA), gibberellic acid (GA3), 2,3,5-triiodobenzoic acid (TIBA)). Results showed no tuber formation in all treatments, but growth and development of treated kohlrabi seedlings was significantly affected in a distinctive manner, with a variety of morphological traits being altered in comparison to matching controls.

scholarly journals Response of cotyledon explants of Capsicum annuum L. cv. kujawianka to chosen plant growth regulators in in vitro culture

2014 ◽  
Vol 64 (1) ◽  
pp. 5-11 ◽  
Author(s):  
Alicja Fraś ◽  
Krystyna Nowak
Keyword(s):  
Plant Growth ◽  
In Vitro Culture ◽  
Capsicum Annuum ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Shoot Buds ◽  
Cotyledon Explants ◽  
Skoog Medium ◽  
Capsicum Annuum L

Shoot buds originated directly on cotyledon explants of <em>Capsicum annuum</em> L. cv. Kujawianka, when Linsmaier and Skoog medium was enriched with BAP (2 mg/l). Kinetin (2 mg/l) or kinetin with IAA (1 mg/l + 1 mg/l) induced indirect shoot buds regeneration from callus. Rooting was obtained with explants cultivated on a medium containing NAA (0,5 mg/l). Occurrence of the early stages of differentiation was proved at the histological level.

scholarly journals Alterations in Microrhizome Induction, Shoot Multiplication and Rooting of Ginger (Zingiber officinale Roscoe) var. Bentong with Regards to Sucrose and Plant Growth Regulators Application

Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 320
Author(s):  
Nisar Ahmad Zahid ◽  
Hawa Z.E. Jaafar ◽  
Mansor Hakiman
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Zingiber Officinale ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Zingiber Officinale Roscoe ◽  
Planting Materials ◽  
Disease Free

Ginger (Zingiber officinale Roscoe) var. Bentong is a monocotyledon plant that belongs to the Zingiberaceae family. Bentong ginger is the most popular cultivar of ginger in Malaysia, which is conventionally propagated by its rhizome. As its rhizomes are the economic part of the plant, the allocation of a large amount of rhizomes as planting materials increases agricultural input cost. Simultaneously, the rhizomes’ availability as planting materials is restricted due to the high demand for fresh rhizomes in the market. Moreover, ginger propagation using its rhizome is accompanied by several types of soil-borne diseases. Plant tissue culture techniques have been applied to produce disease-free planting materials of ginger to overcome these problems. Hence, the in vitro-induced microrhizomes are considered as alternative disease-free planting materials for ginger cultivation. On the other hand, Bentong ginger has not been studied for its microrhizome induction. Therefore, this study was conducted to optimize sucrose and plant growth regulators (PGRs) for its microrhizome induction. Microrhizomes were successfully induced in Murashige and Skoog (MS) medium supplemented with a high sucrose concentration (>45 g L−1). In addition, zeatin at 5–10 µM was found more effective for microrhizome induction than 6-benzylaminopurine (BAP) at a similar concentration. The addition of 7.5 µM 1-naphthaleneacetic acid (NAA) further enhanced microrhizome formation and reduced sucrose’s required dose that needs to be supplied for efficient microrhizome formation. MS medium supplemented with 60 g L−1 sucrose, 10 µM zeatin and 7.5 µM NAA was the optimum combination for the microrhizome induction of Bentong ginger. The in vitro-induced microrhizomes sprouted indoors in moist sand and all the sprouted microrhizomes were successfully established in field conditions. In conclusion, in vitro microrhizomes can be used as disease-free planting materials for the commercial cultivation of Bentong ginger.

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