Sequential study of the synaptonemal complex in rat (Rattus norvegicus) oocytes by light and electron microscopy

Genetica ◽  
1988 ◽  
Vol 77 (3) ◽  
pp. 179-187 ◽  
Author(s):  
R. Pujol ◽  
M. Garcia ◽  
L. Freixa ◽  
J. Egozcue
Keyword(s):  
Electron Microscopy ◽  
Synaptonemal Complex ◽  
Rattus Norvegicus ◽  
Light And Electron Microscopy ◽  
Sequential Study

Sequential study of the synaptonemal complex in Syrian hamster (Mesocricetus auratus) and mouse (Mus musculus) oocytes by light and electron microscopy

Genetica ◽  
1985 ◽  
Vol 67 (2) ◽  
pp. 87-97 ◽  
Author(s):  
L. Freixa ◽  
M. Garcia ◽  
M. Ponsà ◽  
J. Navarro ◽  
J. Egozcue
Keyword(s):  
Electron Microscopy ◽  
Synaptonemal Complex ◽  
Mus Musculus ◽  
Syrian Hamster ◽  
Light And Electron Microscopy ◽  
Mesocricetus Auratus ◽  
Sequential Study

A method for the sequential study of synaptonemal complexes by light and electron microscopy

1981 ◽  
Vol 59 (4) ◽  
pp. 419-421 ◽  
Author(s):  
J. Navarro ◽  
F. Vidal ◽  
M. Guitart ◽  
J. Egozcue
Keyword(s):  
Electron Microscopy ◽  
Light And Electron Microscopy ◽  
Synaptonemal Complexes ◽  
Sequential Study

A TECHNIQUE FOR LIGHT AND ELECTRON MICROSCOPY OF THE SYNAPTONEMAL COMPLEX OF THE MOUSE OOCYTE

1982 ◽  
Vol 24 (6) ◽  
pp. 675-680 ◽  
Author(s):  
Weng Kong Sung ◽  
Georgiana Jagiello
Keyword(s):  
Electron Microscopy ◽  
Synaptonemal Complex ◽  
Microscopic Examination ◽  
Light And Electron Microscopy ◽  
Electron Microscopic Examination ◽  
Mouse Oocyte ◽  
Electron Microscopic ◽  
Synaptonemal Complexes

A method is described for obtaining synaptonemal complex preparations from mouse pachytene oocytes for light and electron microscopic examination. A karyotype based on the whole complement of synaptonemal complexes of a pachytene oocyte as visualized by electron microscopy is presented.

Light and electron microscope observations of a meiotic mutant of Neurospora crassa

1978 ◽  
Vol 56 (21) ◽  
pp. 2694-2706 ◽  
Author(s):  
B.C. Lu ◽  
Donna R. Galeazzi
Keyword(s):  
Electron Microscopy ◽  
Electron Microscope ◽  
Genetic Analysis ◽  
Neurospora Crassa ◽  
Synaptonemal Complex ◽  
Chromosome Pairing ◽  
Light And Electron Microscopy ◽  
Homologous Chromosomes ◽  
Synaptonemal Complexes ◽  
Nuclear Separation

Light and electron microscopy have revealed that the meiotic-1 (mei-1) mutant of Neurospora crassa is defective in chromosome pairing (asynaptic) although plenty of axial components of the synaptonemal complex are produced and occasional tripartite synaptonemal complexes can be formed. The mei-1 mutant is most probably defective in bringing the homologous chromosomes together for pairing and for assembly of the synaptonemal complex. The mei-1 mutant is also defective in nuclear separation which leads to a four-poled spindle at the subsequent division. The lack of chromosome pairing, the incomplete assembly of the synaptonemal complex, and the four-poled spindles account for absence of recombination and for the nondisjunction found in genetic analysis.

Meiotic chromosome structure: relationship between the synaptonemal complex and the chromatid cores

Genome ◽  
1992 ◽  
Vol 35 (6) ◽  
pp. 1054-1061 ◽  
Author(s):  
J. S. Rufas ◽  
J. L. Santos ◽  
M. Diez ◽  
J. A. Suja
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Synaptonemal Complex ◽  
Chromosome Structure ◽  
Sex Chromosome ◽  
Meiotic Chromosome ◽  
Light And Electron Microscopy ◽  
Structure Relationship ◽  
Surface Spread ◽  
Relationship Of

The development of silver-stained synaptonemal complexes (SCs) and of chromatid cores was analyzed in squashed and surface-spread grasshopper spermatocytes using light and electron microscopy, respectively. This study was conducted to determine the relationship of the two chromosome structures and then obtain more insight into the meiotic chromosome structure. Pachytene cells observed by light microscopy showed thin silver-stained threads, representing SCs, along the centre of the bivalents. However, fully formed SCs, and an axial element corresponding to the univalent sex chromosome, appeared when these cells were observed by electron microscopy. During early diplotene no silver-stained threads were observed by light microscopy. However, fragmentation of the SCs was apparent in cells at the same stage when observed by electron microscopy. Both light and electron microscopy showed that chromosome cores were first detected in homologues of late diplotene – early diakinesis cells. During diakinesis the cores were not continuous but were interrupted where interstitial chiasmata occur. In prometaphase I – metaphase I cells these cores appeared continuous and double, i.e., each chromatid clearly showed its own core. We propose a model whereby the associated cores of sister chromatids act as frameworks for the formation of the SC lateral elements.Key words: meiosis, chromosome structure, synaptonemal complex, chromatid core.

Sequential study of synaptonemal complexes in mouse spermatocytes by light and electron microscopy

Genetica ◽  
1985 ◽  
Vol 67 (1) ◽  
pp. 21-30 ◽  
Author(s):  
M. Guitart ◽  
M. D. Coll ◽  
M. Ponsà ◽  
J. Egozcue
Keyword(s):  
Electron Microscopy ◽  
Light And Electron Microscopy ◽  
Synaptonemal Complexes ◽  
Sequential Study

Meiotic chromosome studies and synaptonemal complex analyses by light and electron microscopy in 47 infertile or sterile males

1986 ◽  
Vol 1 (8) ◽  
pp. 523-527 ◽  
Author(s):  
J. Navarro ◽  
F. Vidal ◽  
C. Templado ◽  
J. Benet ◽  
S. Marina ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Synaptonemal Complex ◽  
Meiotic Chromosome ◽  
Light And Electron Microscopy

A method for the sequential study of eimerian chromosomes by light and electron microscopy

2001 ◽  
Vol 94 (3) ◽  
pp. 221-226 ◽  
Author(s):  
E del Cacho ◽  
M Gallego ◽  
L Monteagudo ◽  
F Lopez-Bernad ◽  
J Quilez ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Light And Electron Microscopy ◽  
Sequential Study

The Morphology of Vacuolated Cells in the Liver Following Administration of Vitamin A.

1973 ◽  
Vol 31 ◽  
pp. 408-409
Author(s):  
Odell T. Minick ◽  
Hidejiro Yokoo ◽  
Fawzia Batti
Keyword(s):  
Electron Microscopy ◽  
Body Weight ◽  
Vitamin A ◽  
Light And Electron Microscopy ◽  
Liver Cells ◽  
Prominent Feature ◽  
Vascular Space ◽  
Vacuolated Cell ◽  
Vacuolated Cells ◽  
Vacuolated Cytoplasm

Vacuolated cells in the liver of young rats were studied by light and electron microscopy following the administration of vitamin A (200 units per gram of body weight). Their characteristics were compared with similar cells found in untreated animals.In rats given vitamin A, cells with vacuolated cytoplasm were a prominent feature. These cells were found mostly in a perisinusoidal location, although some appeared to be in between liver cells (Fig. 1). Electron microscopy confirmed their location in Disse's space adjacent to the sinusoid and in recesses between liver cells. Some appeared to be bordering the lumen of the sinusoid, but careful observation usually revealed a tenuous endothelial process separating the vacuolated cell from the vascular space. In appropriate sections, fenestrations in the thin endothelial processes were noted (Fig. 2, arrow).

Control of Autogenous Vein Grafts Prior to Reimplantation, By Electron Microscopy

1972 ◽  
Vol 30 ◽  
pp. 106-107
Author(s):  
John H. L. Watson ◽  
John L. Swedo ◽  
M. Vrandecic
Keyword(s):  
Electron Microscopy ◽  
Light And Electron Microscopy ◽  
Physiological Saline ◽  
Experimental Conditions ◽  
Vein Grafts ◽  
Arterial Blood ◽  
Saphenous Veins ◽  
Autogenous Vein ◽  
Control Of Parameters ◽  
Post Implantation

The ambient temperature and the nature of the storage fluids may well have significant effects upon the post-implantation behavior of venus autografts. A first step in the investigation of such effects is reported here. Experimental conditions have been set which approximate actual operating room procedures. Saphenous veins from dogs have been used as models in the experiments. After removal from the dogs the veins were kept for two hours under four different experimental conditions, viz at either 4°C or 23°C in either physiological saline or whole canine arterial blood. At the end of the two hours they were prepared for light and electron microscopy. Since no obvious changes or damage could be seen in the veins by light microscopy, even with the advantage of tissue specific stains, it was essential that the control of parameters for successful grafts be set by electron microscopy.

Sign in / Sign up

Export Citation Format

Share Document