Genetic analysis of helper virus-specific selective amplification of cucumber mosaic virus satellite RNAs

1995 ◽  
Vol 40 (1) ◽  
pp. 25-29 ◽  
Author(s):  
Marilyn J. Roossinck ◽  
Peter Palukaitis
Keyword(s):  
Genetic Analysis ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Helper Virus ◽  
Selective Amplification ◽  
Satellite Rnas

scholarly journals Cucumber mosaic virus satellite RNAs that induce similar symptoms in melon plants show large differences in fitness

2011 ◽  
Vol 92 (8) ◽  
pp. 1930-1938 ◽  
Author(s):  
Mónica Betancourt ◽  
Aurora Fraile ◽  
Fernando García-Arenal
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Evolutionary Dynamics ◽  
Plant Biomass ◽  
Aphid Transmission ◽  
Helper Virus ◽  
Relative Fitness ◽  
Single Infection ◽  
Trade Offs ◽  
Satellite Rnas

Two groups of Cucumber mosaic virus (CMV) satellite RNAs (satRNAs), necrogenic and non-necrogenic, can be differentiated according to the symptoms they cause in tomato plants, a host in which they also differ in fitness. In most other CMV hosts these CMV-satRNA cause similar symptoms. Here, we analyse whether they differ in traits determining their relative fitness in melon plants, in which the two groups of CMV-satRNAs cause similar symptoms. For this, ten necrogenic and ten non-necrogenic field satRNA genotypes were assayed with Fny-CMV as a helper virus. Neither type of CMV-satRNA modified Fny-CMV symptoms, and both types increased Fny-CMV virulence similarly, as measured by decreases in plant biomass and lifespan. Necrogenic and non-necrogenic satRNAs differed in their ability to multiply in melon tissues; necrogenic satRNAs accumulated to higher levels both in single infection and in competition with non-necrogenic satRNAs. Indeed, multiplication of some non-necrogenic satRNAs was undetectable. Transmission between hosts by aphids was less efficient for necrogenic satRNAs as a consequence of a more severe reduction of CMV accumulation in leaves. The effect of CMV accumulation on aphid transmission was not compensated for by differences in satRNA encapsidation efficiency or transmissibility to CMV progeny. Thus, necrogenic and non-necrogenic satRNAs differ in their relative fitness in melon, and trade-offs are apparent between the within-host and between-host components of satRNA fitness. Hence, CMV-satRNAs could have different evolutionary dynamics in CMV host-plant species in which they do not differ in pathogenicity.

scholarly journals Characterization of Synergy Between Cucumber mosaic virus and Potyviruses in Cucurbit Hosts

2002 ◽  
Vol 92 (1) ◽  
pp. 51-58 ◽  
Author(s):  
Yongzeng Wang ◽  
Victor Gaba ◽  
Jie Yang ◽  
Peter Palukaitis ◽  
Amit Gal-On
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Mixed Infection ◽  
Zucchini Yellow Mosaic Virus ◽  
Helper Virus ◽  
Synergistic Interaction ◽  
Yellow Mosaic Virus ◽  
Watermelon Mosaic Virus ◽  
Satellite Rnas ◽  
Rna Levels

Mixed infections of cucurbits by Cucumber mosaic virus (CMV) and potyviruses exhibit a synergistic interaction. Zucchini squash and melon plants coinfected by the potyvirus Zucchini yellow mosaic virus (ZYMV) and either Fny-CMV (subgroup IA) or LS-CMV (subgroup II) displayed strong synergistic pathological responses, eventually progressing to vascular wilt and plant death. Accumulation of Fny- or LS-CMV RNAs in a mixed infection with ZYMV in zucchini squash was slightly higher than infection with CMV strains alone. There was an increase in CMV (+) strand RNA levels, but no increase in CMV (-) RNA3 levels during mixed infection with ZYMV. Moreover, only the level of capsid protein from LS-CMV increased in mixed infection. ZYMV accumulated to similar levels in singly and mixed infected zucchini squash and melon plants. Coinfection of squash with the potyvirus Watermelon mosaic virus (WMV) and CMV strains increased both the Fny-CMV RNA levels and the LS-CMV RNA levels. However, CMV (-) strand RNA3 levels were increased little or not at all for CMV on coinfection with WMV. Infection of CMV strains (LS and Fny) containing satellite RNAs (WL47-sat RNA and B5*-sat RNA) reduced the accumulation of the helper virus RNA, except when B5*-sat RNA was mixed with LS- CMV. However, mixed infection containing ZYMV and the CMV strains with satellites reversed the suppression effect of satellite RNAs on helper virus accumulation and increased satellite RNA accumulation. The synergistic interaction between CMV and potyviruses in cucurbits exhibited different features from that documented in tobacco, indicating there are differences in the mechanisms of potyvirus synergistic phenomena.

scholarly journals Characterization of cucumber mosaic virus and its satellite RNAs associated with tomato lethal necrosis in Serbia

2021 ◽  
Author(s):  
Ivana Stanković ◽  
Ana Vučurović ◽  
Katarina Zečević ◽  
Branka Petrović ◽  
Dušan Nikolić ◽  
...  
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Satellite Rnas

scholarly journals Transmissibility of Cucumber mosaic virus by Aphis gossypii Correlates with Viral Accumulation and Is Affected by the Presence of Its Satellite RNA

2000 ◽  
Vol 90 (10) ◽  
pp. 1068-1072 ◽  
Author(s):  
Fernando Escriu ◽  
Keith L. Perry ◽  
Fernando García-Arenal
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Aphis Gossypii ◽  
Natural Populations ◽  
Virus Transmission ◽  
Negative Effect ◽  
Infected Tomato ◽  
Satellite Rnas ◽  
Viral Accumulation ◽  
Test Plants

Satellite RNAs (satRNAs) are associated with Cucumber mosaic virus (CMV) in tomato, most often causing severe epidemics of necrotic plants, and not associated with specific host symptoms. Laboratory studies on virus transmission by the aphid vector Aphis gossypii were performed to better understand the dynamics of field populations of CMV. The presence of satRNAs correlated with lower concentrations of virus in infected plants and with a decrease in the efficiency of transmission from satRNA-infected plants. Both the concentration of virus in CMV-infected tomato and the efficiency of transmission varied more extensively with nonnecrogenic satRNAs than with necrogenic satRNAs. A negative effect of satRNAs on virus accumulation can account, in part, for a decrease in the field transmission and recovery of CMV + satRNAs. Aphids behaved differently and probed less readily on plants infected with CMV + necrogenic satRNAs compared with plants containing non-necrogenic satRNAs. Aphid-mediated satRNA-free CMV infections were observed in test plants when aphids were fed on source plants containing CMV + nonnecrogenic satRNA; no comparable satRNA-free test plants occurred when aphids were fed on source plants containing necrogenic satRNAs. These results indicate that factors associated with transmission can be a determinant in the evolution of natural populations of CMV and its satRNA.

Differential interactions among strains of tomato aspermy virus and satellite RNAs of cucumber mosaic virus

Virology ◽  
1992 ◽  
Vol 186 (2) ◽  
pp. 475-480 ◽  
Author(s):  
Enrique Moriones ◽  
Isabel Diaz ◽  
Emilio Rodriguez-Cerezo ◽  
Aurora Fraile ◽  
Fernando Garcia-Arenal
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Tomato Aspermy Virus ◽  
Satellite Rnas

scholarly journals Emergence and Characterization of Satellite RNAs Associated with Spanish Cucumber Mosaic Virus Isolates

1984 ◽  
Vol 65 (3) ◽  
pp. 539-547 ◽  
Author(s):  
I. Garcia-Luque ◽  
J. M. Kaper ◽  
J. R. Diaz-Ruiz ◽  
M. Rubio-Huertos
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Satellite Rnas ◽  
Virus Isolates

scholarly journals Impact of a defective RNA 3 from cucumber mosaic virus on helper virus infection dynamics

Virology ◽  
2009 ◽  
Vol 389 (1-2) ◽  
pp. 59-65 ◽  
Author(s):  
Minoru Takeshita ◽  
Yuka Matsuo ◽  
Masashi Suzuki ◽  
Naruto Furuya ◽  
Kenichi Tsuchiya ◽  
...  
Keyword(s):  
Virus Infection ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Helper Virus ◽  
Infection Dynamics ◽  
Defective Rna
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