Effect of nitrate pulses on the nitrate-uptake rate, synthesis of mRNA coding for nitrate reductase, and nitrate-reductase activity in the roots of barley seedlings

Planta ◽  
1993 ◽  
Vol 189 (4) ◽  
Author(s):  
Rudolf Tischner ◽  
Barbara Waldeck ◽  
ShamS. Goyal ◽  
WilliamD. Rains
Keyword(s):  
Nitrate Reductase ◽  
Uptake Rate ◽  
Nitrate Reductase Activity ◽  
Nitrate Uptake ◽  
Reductase Activity ◽  
Nitrate Uptake Rate

scholarly journals Physiological response of a golden tide alga (<i>Sargassum muticum</i>) to the interaction of ocean acidification and phosphorus enrichment

2017 ◽  
Vol 14 (3) ◽  
pp. 671-681 ◽  
Author(s):  
Zhiguang Xu ◽  
Guang Gao ◽  
Juntian Xu ◽  
Hongyan Wu
Keyword(s):  
Nitrate Reductase ◽  
Ocean Acidification ◽  
Soluble Protein ◽  
Nitrate Reductase Activity ◽  
Nitrate Uptake ◽  
Dark Respiration ◽  
Reductase Activity ◽  
Soluble Carbohydrates ◽  
Promoting Effect ◽  
Nitrate Uptake Rate

Abstract. The development of golden tides is potentially influenced by global change factors, such as ocean acidification and eutrophication, but related studies are very scarce. In this study, we cultured a golden tide alga, Sargasssum muticum, at two levels of pCO2 (400 and 1000 µatm) and phosphate (0.5 and 40 µM) to investigate the interactive effects of elevated pCO2 and phosphate on the physiological properties of the thalli. Higher pCO2 and phosphate (P) levels alone increased the relative growth rate by 41 and 48 %, the net photosynthetic rate by 46 and 55 %, and the soluble carbohydrates by 33 and 62 %, respectively, while the combination of these two levels did not promote growth or soluble carbohydrates further. The higher levels of pCO2 and P alone also enhanced the nitrate uptake rate by 68 and 36 %, the nitrate reductase activity (NRA) by 89 and 39 %, and the soluble protein by 19 and 15 %, respectively. The nitrate uptake rate and soluble protein was further enhanced, although the nitrate reductase activity was reduced when the higher levels of pCO2 and P worked together. The higher pCO2 and higher P levels alone did not affect the dark respiration rate of the thalli, but together they increased it by 32 % compared to the condition of lower pCO2 and lower P. The neutral effect of the higher levels of pCO2 and higher P on growth and soluble carbohydrates, combined with the promoting effect on soluble protein and dark respiration, suggests that more energy was drawn from carbon assimilation to nitrogen assimilation under conditions of higher pCO2 and higher P; this is most likely to act against the higher pCO2 that caused acid–base perturbation via synthesizing H+ transport-related protein. Our results indicate that ocean acidification and eutrophication may not boost golden tide events synergistically, although each one has a promoting effect.

Nitrate Uptake and the Induction of Nitrate Reductase Activity in Wheat (Triticum aestivumL.) Seedlings

1981 ◽  
Vol 32 (1) ◽  
pp. 9-18 ◽  
Author(s):  
P. W. JONES ◽  
C. B. JOHNSON ◽  
W. J. WHITTINGTON
Keyword(s):  
Nitrate Reductase ◽  
Nitrate Reductase Activity ◽  
Nitrate Uptake ◽  
Reductase Activity

scholarly journals Effects of Nitrite, Chlorate, and Chlorite on Nitrate Uptake and Nitrate Reductase Activity

1992 ◽  
Vol 100 (2) ◽  
pp. 644-650 ◽  
Author(s):  
M. Yaeesh Siddiqi ◽  
Bryan J. King ◽  
Anthony D. M. Glass
Keyword(s):  
Nitrate Reductase ◽  
Nitrate Reductase Activity ◽  
Nitrate Uptake ◽  
Reductase Activity

scholarly journals Induction of nitrate uptake and nitrate reductase activity in trembling aspen and lodgepole pine

1998 ◽  
Vol 21 (10) ◽  
pp. 1039-1046 ◽  
Author(s):  
X. Min ◽  
M. Y. Siddiqi ◽  
R. D. Guy ◽  
A. D. M. Glass ◽  
H. J. Kronzucker
Keyword(s):  
Nitrate Reductase ◽  
Nitrate Reductase Activity ◽  
Nitrate Uptake ◽  
Lodgepole Pine ◽  
Reductase Activity ◽  
Trembling Aspen

Effects of Glufosinate on Anion Uptake in Lemna gibba G 1

1989 ◽  
Vol 44 (1-2) ◽  
pp. 33-38 ◽  
Author(s):  
Gudrun D. Trogisch ◽  
Helmut Köcher ◽  
Wolfram R. Ullrich
Keyword(s):  
Nitrate Reductase ◽  
Nitrate Reductase Activity ◽  
Nitrate Uptake ◽  
Dark Respiration ◽  
Reductase Activity ◽  
Lemna Gibba ◽  
Proton Cotransport ◽  
Chlorophyll Formation

Abstract The duckweed Lemna gibba G 1 was used as a model to study inhibitory sites with the herbicide and glutamate analogue glufosinate (PPT). Growth and chlorophyll formation were partly inhibited by 25 n-M, completely suppressed by 250 (im PPT. Photosynthesis showed partial inhibition within few hours, dark respiration ( 0 2 consumption) increased already within one hour. In the presence of 1 mM PPT in the light, the ammonium pool of Lemna increased to 600% within few hours, later to 1000%. The overall amino acid pool exhibited a slower increase to 300%, the nitrate pool only a slight increase, while total phosphate remained almost unchanged. In the dark all these effects were less pronounced than in the light. Nitrate, nitrite and phosphate uptake were partially inhibited by PPT, especially after 19 h PPT pretreatment. Nitrate reductase activity in vitro, after PPT treatment in vivo, showed an inhibition similar to that of nitrate uptake. Ammonium was not taken up but released under the same conditions. The data are explained by a combined effect of PPT, by inhibition of glutamine synthetase leading to accumulation of ammonium from photorespiration and proteolysis, by membrane depolarization and inhibition of anion/proton cotransport, by secondary uncoupling of phosphorylation, and by secondary inhibition of nitrate reductase activity.

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