Growth and thiophene accumulation by hairy root cultures of Tagetes patula in media of varying initial pH

1991 ◽  
Vol 9 (11) ◽  
Author(s):  
Usha Mukundan ◽  
MartinA. Hjortso
Keyword(s):  
Hairy Root ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Tagetes Patula ◽  
Initial Ph

Production of nematocidal compounds by hairy root cultures of Tagetes patula L.

1990 ◽  
Vol 9 (7) ◽  
Author(s):  
Masaharu Kyo ◽  
Yukio Miyauchi ◽  
Tadaaki Fujimoto ◽  
Shigeyuki Mayama
Keyword(s):  
Hairy Root ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Tagetes Patula

scholarly journals Optimization of conditions for the induction of Tagetes patula L. hairy roots

2019 ◽  
Vol 9 (3) ◽  
pp. 415-420
Author(s):  
L. P. Khlebova ◽  
E. S. Brovko ◽  
O. V. Bychkova ◽  
N. V. Pavlova
Keyword(s):  
Hairy Root ◽  
Hairy Roots ◽  
Wild Strain ◽  
Leaf Explants ◽  
Biologically Active ◽  
Infected Leaf ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Tagetes Patula ◽  
Time Of Infection

The hairy root cultures are promising sources of secondary metabolites of plants, including rare and endangered species. They possess genetic and biochemical stability, unlimited growth rate in free-hormone medium, short doubling times, high biosynthetic activity and ecological purity of plant raw materials. The hairy root cultures of Tagetes patula L. can be used to produce biologically active substances with biocidal activity. The study aimed to determine the virulent strain of Agrobacterium rhizogenes and the most effective period of co-cultivation of T. patula leaf explants with an agrobacterium to induce actively growing hairy root cultures. We used 3 strains (A-4b, 8196RT and 15834). The time of infection ranged from 3 to 33 hours in increments of 3 hours. We found that 24 h is the best time of infection to induce hairy roots with the highest transformation efficiency (92%). The wild strain A. rhizogenes 15834 turned out to be the most virulent when infected leaf explants of spreading marigold. This strain provided the maximum transformation effect, reaching 85.4%. We have identified 5 actively growing clones of hairy roots with intensive branching, the growth indices of which were 64-75. In the future, they will be transferred to a liquid medium for biomass accumulation and scaling.

Growth and thiophene production by hairy root cultures of Tagetes patula in various two-liquid-phase bioreactors

1991 ◽  
Vol 13 (6) ◽  
pp. 487-494 ◽  
Author(s):  
R.M. Buitelaar ◽  
A.A.M. Langenhoff ◽  
R. Heidstra ◽  
J. Tramper
Keyword(s):  
Liquid Phase ◽  
Hairy Root ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Tagetes Patula

NMR Spectral Data of Benzofurans and Bithiophenes from Hairy Root Cultures of Tagetes Patula Andthe Molecular Structure of Isoeuparin

1991 ◽  
Vol 24 (10) ◽  
pp. 1405-1413 ◽  
Author(s):  
Marios A. Menelaou ◽  
Frank R. Fronczek ◽  
Martin A. Hjortso ◽  
Anne F. Morrison ◽  
Maryam Foroozesh ◽  
...  
Keyword(s):  
Molecular Structure ◽  
Spectral Data ◽  
Hairy Root ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Tagetes Patula

Biosynthetic Studies of Bithiophenes in Hairy Root Cultures of Tagetes Patula Using13C-labeled Acetates

1991 ◽  
Vol 24 (3) ◽  
pp. 353-370 ◽  
Author(s):  
Marios A. Menelaou ◽  
David Vargas ◽  
Nikolaus H. Fischer ◽  
Maryam Foroozesh ◽  
Tina M. Thibodeaux ◽  
...  
Keyword(s):  
Hairy Root ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Tagetes Patula ◽  
Biosynthetic Studies

scholarly journals Effect of exogenous auxin on root morphology and secondary metabolism in Tagetes patula hairy root cultures

1995 ◽  
Vol 93 (2) ◽  
pp. 233-240 ◽  
Author(s):  
R. R. J. Arroo ◽  
A. Develi ◽  
H. Meijers ◽  
E. Westerlo ◽  
A. K. Kemp ◽  
...  
Keyword(s):  
Secondary Metabolism ◽  
Hairy Root ◽  
Root Morphology ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Tagetes Patula ◽  
Exogenous Auxin

scholarly journals Effect of exogenous auxin on root morphology and secondary metabolism in Tagetes patula hairy root cultures

1995 ◽  
Vol 93 (2) ◽  
pp. 233-240 ◽  
Author(s):  
R. R. J. Arroo ◽  
A. Develi ◽  
H. Meijers ◽  
E. Van de Westerlo ◽  
A. K. Kemp ◽  
...  
Keyword(s):  
Secondary Metabolism ◽  
Hairy Root ◽  
Root Morphology ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Tagetes Patula ◽  
Exogenous Auxin
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