High-frequency plant regeneration from seed-derived callus cultures of Kentucky bluegrass (Poa pratensis L.)

Genetic transformation of Kentucky bluegrass (Poa pratensis L.) requires methods for high-frequency regeneration of plantlets from cultured cells. Regeneration of this important turf species has been reported, albeit at a low frequency from seed-derived callus. We tested the potential of 3 synthetic auxins, used in the callus initiation and growth medium, for their ability to promote regeneration in 3 bluegrass varieties. 10 μM 2,4-D promoted regeneration from 0 to 5% of calli, 30 μM and 60 μM picloram promoted regeneration from 0 to 8% of calli, and 10 to 60 μM dicamba, in combination with BA, promoted regeneration from 1% to 8% of calli. In a subsequent experiment, both 60μM and 90 μM dicamba, with 20 μM BA, promoted regeneration from 45% of calli averaged across varieties. These media were tested for the promotion of regeneration in 12 diverse bluegrass varieties. Although up to 45% of the calli from some varieties regenerated plantlets, the response of other varieties was markedly lower, indicating a genetic component in the response to these media.

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High-frequency Plant Regeneration from Mature Seed-derived Callus Cultures of Orchardgrass

Journal of Plant Biotechnology ◽

10.5010/jpb.2003.30.4.341 ◽

2003 ◽

Vol 30 (4) ◽

pp. 341-346 ◽

Cited By ~ 8

Keyword(s):

Plant Regeneration ◽

High Frequency ◽

Callus Cultures ◽

Mature Seed

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HIGH FREQUENCY PLANT REGENERATION FROM CALLUS CULTURES DERIVED FROM PRIMORDIAL LEAVES OF ADULT JAPANESE PERSIMMON AND PROTOPLAST ISOLATION FROM THOSE CALLUS LINES

Acta Horticulturae ◽

10.17660/actahortic.1992.300.35 ◽

1992 ◽

pp. 251-254

Author(s):

R. TAO ◽

K. YONEMORI ◽

A. SUGIURA

Keyword(s):

Plant Regeneration ◽

High Frequency ◽

Protoplast Isolation ◽

Callus Cultures ◽

Japanese Persimmon ◽

Callus Lines

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582 PB 091 PLANT REGENERATION FROM EMBRYOGENIC CALLUS TISSUES OF KENTUCKY BLUEGRASS

HortScience ◽

10.21273/hortsci.29.5.515c ◽

1994 ◽

Vol 29 (5) ◽

pp. 515c-515

Author(s):

Shanqiang Ke ◽

Chiwon W. Lee

Keyword(s):

Plant Regeneration ◽

Light Exposure ◽

Poa Pratensis ◽

Shoot Proliferation ◽

Kentucky Bluegrass ◽

Ms Medium ◽

Embryogenic Calli ◽

Albino Plants ◽

Proliferation Medium ◽

Callus Tissues

Coleoptile tissues from dark-germinated seedlings of Kentucky bluegrass (Poa pratensis L.) cv. Touchdown were excised and cultured on MS medium supplemented with 1.5-2.5 mg/liter picloram plus 0.2 mg/liter benzyladenine (BA) or with 4 mg/liter 2,4-D. Embryogenic calli were formed on media containing 1.5 mg/liter picloram plus 2.5 mg/liter 2,4-D in the dark. When these embryogenic calli were subcultured on MS medium containing either 0.15-0.3 mg/liter picloram or 0.2-0.5 mg/liter 2,4-D in a 16-h day/8-h night photoperiod, 10.5% of the cultures regenerated shoots. Pretreatment of cultures in the dark for 2 weeks prior to light exposure slightly increased the plant regeneration efficiency to 15.5%. Pigmentation of the regenerants varied with a ratio of 8.5 completely green: 2.5 green plus albino: 1 completely albino plants. The shoots were multiplied in the medium containing 0.5 mg/liter BA plus either 0.2 mg/liter picloram or 0.1 mg/liter indoleacetic acid (IAA). Over 90% cultures in the shoot proliferation medium produced roots after 4 weeks.

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