High-frequency plant regeneration from seed-derived callus cultures of Kentucky bluegrass (Poa pratensis L.)

1995 ◽  
Vol 14 (11) ◽  
Author(s):  
JeffreyD. Griffin ◽  
MargaretS. Dibble
Keyword(s):  
Plant Regeneration ◽  
High Frequency ◽  
Poa Pratensis ◽  
Kentucky Bluegrass ◽  
Callus Cultures

scholarly journals 585 PB 100 IMPROVED REGENERATION FREQUENCY OF KENTUCKY BLUEGRASS FROM CALLUS CULTURES

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 515f-515
Author(s):  
Jeffrey D. Griffin ◽  
Margaret S. Dibble
Keyword(s):  
High Frequency ◽  
Cultured Cells ◽  
Poa Pratensis ◽  
Low Frequency ◽  
Kentucky Bluegrass ◽  
Callus Cultures ◽  
Regeneration Frequency ◽  
Synthetic Auxins ◽  
Callus Initiation ◽  
Regenerated Plantlets

Genetic transformation of Kentucky bluegrass (Poa pratensis L.) requires methods for high-frequency regeneration of plantlets from cultured cells. Regeneration of this important turf species has been reported, albeit at a low frequency from seed-derived callus. We tested the potential of 3 synthetic auxins, used in the callus initiation and growth medium, for their ability to promote regeneration in 3 bluegrass varieties. 10 μM 2,4-D promoted regeneration from 0 to 5% of calli, 30 μM and 60 μM picloram promoted regeneration from 0 to 8% of calli, and 10 to 60 μM dicamba, in combination with BA, promoted regeneration from 1% to 8% of calli. In a subsequent experiment, both 60μM and 90 μM dicamba, with 20 μM BA, promoted regeneration from 45% of calli averaged across varieties. These media were tested for the promotion of regeneration in 12 diverse bluegrass varieties. Although up to 45% of the calli from some varieties regenerated plantlets, the response of other varieties was markedly lower, indicating a genetic component in the response to these media.

scholarly journals 582 PB 091 PLANT REGENERATION FROM EMBRYOGENIC CALLUS TISSUES OF KENTUCKY BLUEGRASS

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 515c-515
Author(s):  
Shanqiang Ke ◽  
Chiwon W. Lee
Keyword(s):  
Plant Regeneration ◽  
Light Exposure ◽  
Poa Pratensis ◽  
Shoot Proliferation ◽  
Kentucky Bluegrass ◽  
Ms Medium ◽  
Embryogenic Calli ◽  
Albino Plants ◽  
Proliferation Medium ◽  
Callus Tissues

Coleoptile tissues from dark-germinated seedlings of Kentucky bluegrass (Poa pratensis L.) cv. Touchdown were excised and cultured on MS medium supplemented with 1.5-2.5 mg/liter picloram plus 0.2 mg/liter benzyladenine (BA) or with 4 mg/liter 2,4-D. Embryogenic calli were formed on media containing 1.5 mg/liter picloram plus 2.5 mg/liter 2,4-D in the dark. When these embryogenic calli were subcultured on MS medium containing either 0.15-0.3 mg/liter picloram or 0.2-0.5 mg/liter 2,4-D in a 16-h day/8-h night photoperiod, 10.5% of the cultures regenerated shoots. Pretreatment of cultures in the dark for 2 weeks prior to light exposure slightly increased the plant regeneration efficiency to 15.5%. Pigmentation of the regenerants varied with a ratio of 8.5 completely green: 2.5 green plus albino: 1 completely albino plants. The shoots were multiplied in the medium containing 0.5 mg/liter BA plus either 0.2 mg/liter picloram or 0.1 mg/liter indoleacetic acid (IAA). Over 90% cultures in the shoot proliferation medium produced roots after 4 weeks.

Optimizing plant regeneration from seed-derived callus cultures of Kentucky bluegrass. The effect of benzyladenine

1995 ◽  
Vol 40 (1) ◽  
pp. 101-103 ◽  
Author(s):  
P. van der Valk ◽  
F. Ruis ◽  
A. M. Tettelaar-Schrier ◽  
C. M. van de Velde
Keyword(s):  
Plant Regeneration ◽  
Kentucky Bluegrass ◽  
Callus Cultures

Plant regeneration in Kentucky bluegrass (Poa pratensis L.) via coleoptile tissue cultures

1996 ◽  
Vol 15 (12) ◽  
pp. 882-887 ◽  
Author(s):  
Shanqiang Ke ◽  
Chiwon W. Lee
Keyword(s):  
Plant Regeneration ◽  
Poa Pratensis ◽  
Kentucky Bluegrass ◽  
Tissue Cultures

scholarly journals Plant Regeneration from Seed-derived Callus in Kentucky Bluegrass(Poa pratensis L.)

2004 ◽  
Vol 24 (3) ◽  
pp. 265-270
Author(s):  
Ho-Sung Yoon ◽  
Myunghee Lee ◽  
Eunkyung Bae ◽  
Hyoshin Lee ◽  
Jinki Jo
Keyword(s):  
Plant Regeneration ◽  
Poa Pratensis ◽  
Kentucky Bluegrass

High frequency somatic embryogenesis and plant regeneration from zygotic embryo-derived callus cultures of three Allium species

1992 ◽  
Vol 30 (3) ◽  
pp. 181-191 ◽  
Author(s):  
P. van der Valk ◽  
O. E. Scholten ◽  
F. Verstappen ◽  
R. C. Jansen ◽  
J. J. M. Dons
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
High Frequency ◽  
Zygotic Embryo ◽  
Callus Cultures ◽  
Allium Species
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