scholarly journals 582 PB 091 PLANT REGENERATION FROM EMBRYOGENIC CALLUS TISSUES OF KENTUCKY BLUEGRASS

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 515c-515
Author(s):  
Shanqiang Ke ◽  
Chiwon W. Lee
Keyword(s):  
Plant Regeneration ◽  
Light Exposure ◽  
Poa Pratensis ◽  
Shoot Proliferation ◽  
Kentucky Bluegrass ◽  
Ms Medium ◽  
Embryogenic Calli ◽  
Albino Plants ◽  
Proliferation Medium ◽  
Callus Tissues

Coleoptile tissues from dark-germinated seedlings of Kentucky bluegrass (Poa pratensis L.) cv. Touchdown were excised and cultured on MS medium supplemented with 1.5-2.5 mg/liter picloram plus 0.2 mg/liter benzyladenine (BA) or with 4 mg/liter 2,4-D. Embryogenic calli were formed on media containing 1.5 mg/liter picloram plus 2.5 mg/liter 2,4-D in the dark. When these embryogenic calli were subcultured on MS medium containing either 0.15-0.3 mg/liter picloram or 0.2-0.5 mg/liter 2,4-D in a 16-h day/8-h night photoperiod, 10.5% of the cultures regenerated shoots. Pretreatment of cultures in the dark for 2 weeks prior to light exposure slightly increased the plant regeneration efficiency to 15.5%. Pigmentation of the regenerants varied with a ratio of 8.5 completely green: 2.5 green plus albino: 1 completely albino plants. The shoots were multiplied in the medium containing 0.5 mg/liter BA plus either 0.2 mg/liter picloram or 0.1 mg/liter indoleacetic acid (IAA). Over 90% cultures in the shoot proliferation medium produced roots after 4 weeks.

scholarly journals Somatic embryogenesis of hypocotyl derived calli from an eggplant cultivar

2020 ◽  
Vol 115 (1) ◽  
pp. 151
Author(s):  
Hajar SABET ◽  
Mahmood MALEKI ◽  
Maryam ABDOLI NASAB ◽  
Saeid MIRZAEI
Keyword(s):  
Plant Regeneration ◽  
Culture Medium ◽  
Shoot Elongation ◽  
Gene Transformation ◽  
Ms Medium ◽  
Embryogenic Calli ◽  
Hypocotyl Explants ◽  
Somaclonal Variations ◽  
Micro Propagation ◽  
Embryogenic Callus Induction

<p>Optimization of tissue culture and regeneration conditions of eggplant is necessary for achieving different goals such as gene transformation and the development of somaclonal variations. In this study, hypocotyl explants ware used to produce callus in a medium containing different concentrations of NAA and BAP. Moreover, the concentration of the elements Ca, Mn, Mg, Fe and K were measured and analysed between embryogenic and non-embryogenic calli. For shoot elongation, embryogenic calli were transferred to a new culture medium containing 3.5, 4 and 4.5 mg l<sup>-1</sup> BAP plus 2 mg l<sup>-1</sup> GA3. Finally, produced shoots were rooted in a culture medium containing 1, 1.5 and 2 mg l<sup>-1</sup> NAA. Results showed that the best treatment for the embryogenic callus induction was MS medium containing 0.5 mg l<sup>-1</sup> BAP plus 0.25 mg l<sup>-1</sup> NAA. Two elements, Fe and K, had the highest amount in non-embryogenic calli compare to the embryogenic one. For plant regeneration, MS medium containing 4.5 mg l<sup>-1</sup> BAP plus 2 mg l<sup>-1</sup> GA3 and 2 mg l<sup>-1</sup> NAA were the best treatments for shooting and rooting, respectively. In this study, the best treatments for plant regeneration produced 35 shoots from an explant with 92 % shooting. This regeneration protocol could be useful for gene transformation and micro-propagation studies.</p>

scholarly journals An efficient regeneration system through in vitro somatic embryogenesis of barley (Hordeum vulgare L.)

2019 ◽  
Vol 27 ◽  
pp. 89-99
Author(s):  
M Haque ◽  
SMS Islam
Keyword(s):  
Plant Regeneration ◽  
Callus Induction ◽  
Casein Hydrolysate ◽  
Ms Medium ◽  
Regeneration System ◽  
Embryogenic Calli ◽  
Hordeum Vulgare L ◽  
The Media ◽  
Barley Genotypes

This study was carried out to improve an efficient protocol for in vitro callus induction and plant regeneration using Bangladeshi barley genotypes collected from BARI, Gazipur, Bangladesh. After sterilization embryos were separated carefully from mature seeds of six barley genotypes (BB-1, BB-2, BB-3, BB-4, BB-5 and BB-6) and cultured them in MS medium supplemented with various concentration and combination of PGRs for callus induction and regeneration. Out of six genotypes BB-6 showed highest (38.17%) callus induction in MS + 4.0 mg/l 2,4-D + 200 mg/l L-proline + 300 mg/l casein hydrolysate; whereas, BB-4 and BB-5 showed no callus induction in the same medium. For plant regeneration from embryogenic calli the same genotype (BB-6) also performed the best results (19.25%) in MS medium supplemented with 1.5 mg/l BAP + 30 g/l sucrose. Analysis of variance (ANOVA) showed highly significant differences among the media and the genotypes. J. bio-sci. 27: 89-99, 2019

scholarly journals Influence of the rolC Gene on Proteins Associated with Stroma and Thylakoid Membranes of Chloroplasts in Transgenic Plants of Kentucky Bluegrass

HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 482D-482
Author(s):  
Shanqiang Ke ◽  
Chiwon W. Lee ◽  
Murray E. Duysen
Keyword(s):  
Transgenic Plants ◽  
Poa Pratensis ◽  
Kentucky Bluegrass ◽  
Thylakoid Membranes ◽  
Induction Medium ◽  
Protein Accumulation ◽  
Naphthaleneacetic Acid ◽  
Plastid Development ◽  
Ps Ii ◽  
Albino Plants

The effects of the expression of the rolC gene on protein accumulation in the chloroplasts of transgenic Kentucky bluegrass (Poa pratensis L.) were investigated. Coleoptile tissues excised from 3-day dark-grown seedlings were bombarded with tungsten particles coated with DNA of the engineered plasmid, pGA-GUSGF, containing the npt II, gus, and rolC genes. The tissues were cultured on callus induction medium, which consists of MS salts supplemented with 0.2 mg/L picloram, 0.01 mg/L naphthaleneacetic acid (NAA) 250 mg/L kanamycin, and 100 mM acetosyringone. The putative transformants were either albinos or variegated plants composed of white and green sections. These albino plants had little or no stroma-based 56-kDa and 14-kDa subunits of the suspected Rubisco proteins, which are expressed in response to genes in the nucleus and plastid, respectively. The albino plants also lacked the 110-kDa and 57–58-kDa, and 43, 47-kDa polypeptides in PS I, coupling factor, and PS II in thylakoid membranes, respectively. These proteins involved in photosynthesis are translated from plastidbased genes. No light-harvesting complex proteins (LHC) were observed in these albino plants. LHC genes are encoded in the nucleus. The thylakoid membrane proteins in the chloroplasts of the rolC transgenic variegated plants contained these proteins. Our data suggest that the nucleus and plastid gene products for plastid development are concomitantly impaired by expression of genes in the transgenic plants.

scholarly journals In vitro Shoot Proliferation and Plant Regeneration of Physalis minima L. - a Perennial Medicinal Herb

2010 ◽  
Vol 44 (4) ◽  
pp. 453-456 ◽  
Author(s):  
Farhana Afroz ◽  
AKM Sayeed Hassan ◽  
Laila Shamroze Bari ◽  
Rebeka Sultana ◽  
Nadira Begum ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Room Temperature ◽  
Shoot Proliferation ◽  
Nodal Segments ◽  
Nodal Segment ◽  
Ms Medium ◽  
Regenerated Plants ◽  
Half Strength ◽  
Shoot Tip Explants

The present study describes a protocol for high frequency plant regeneration of Physalis minima. Shoots were induced by culturing nodal segments and shoot tips from 15 day old seedlings. About 29 and 32 shoots were found to be induced from nodal segment and shoot tip explants, respectively, cultured on MS medium supplemented with 1.0 mg/l BAP. When shoots were subcultured on the fresh medium with same component as mentioned above, the shoots were elongated. Shoots rooted well when they were excised individually and implanted on half-strength MS medium with 0.3 mg/l NAA, where 98% shoots rooted within 12-15 days. In vitro grown plantlets with strong root system were successfully established in normal room temperature for seven days before transplanting in pots where they were reared for three weeks through successive acclimatization. The regenerated plants were successfully transferred to the soil with 90% survival rate. Key words: Physalis minima; Medicinal plant; Shoot proliferation; Micropropagation; Regeneration DOI: 10.3329/bjsir.v44i4.4597 Bangladesh J. Sci. Ind. Res. 44(4), 453-456, 2009

scholarly journals Plant regeneration from protoplasts of Gentiana straminea Maxim

2016 ◽  
Vol 11 (1) ◽  
pp. 55-60 ◽  
Author(s):  
Guomin Shi ◽  
Lina Yang ◽  
Tao He
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Somatic Embryos ◽  
Protoplast Culture ◽  
Dichlorophenoxyacetic Acid ◽  
Ms Medium ◽  
Fresh Weight ◽  
Embryogenic Calli ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Solid Liquid

AbstractA protocol is described for plant regeneration from protoplasts of Gentiana straminea Maxim. via somatic embryogenesis. Protoplasts were isolated from embryogenic calli in an enzyme solution composed of 2% Cellulase Onozuka R-10, 0.5% Macerozyme R-10, 0.5% Hemicellulase, and 0.5 M sorbitol with a yield of 3.0 × 106 protoplasts per gram of fresh weight. Liquid, solid-liquid double layer (sLD) and agar-pool (aPL) culture systems were used for protoplast culture. The aPL culture was the only method that produced embryogenic, regenerative calli. With aPL culture, the highest frequencies of protoplast cell division and colony formation were 39.6% and 16.9%, respectively, on MS medium supplemented with 2 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5 mg/L N6-benzylaminopurine (BA). Microcalli were transferred to solid MS medium containing a reduced concentration of 2,4-D (0.5 mg/L) to promote the formation of embryogenic calli. Somatic embryos developed into plantlets on MS medium supplemented with 2 mg/L BA at a rate of 43.7%.

scholarly journals Plant regeneration through somatic embryogenesis of yacón [smallanthus sonchifolius (poepp. and endl.) H. Robinson]

2009 ◽  
Vol 52 (3) ◽  
pp. 549-554 ◽  
Author(s):  
Cynthia Manyra Corrêa ◽  
Graciele Nicolodi de Oliveira ◽  
Leandro Vieira Astarita ◽  
Eliane Romanato Santarém
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Somatic Embryos ◽  
Ms Medium ◽  
Embryogenic Calli ◽  
Efficient System ◽  
Smallanthus Sonchifolius ◽  
Tuberous Roots ◽  
Medicinal Use ◽  
Half Strength

Smallanthus sonchifolius has tuberous roots containing large amounts of fructo-oligosaccharides and its medicinal use has increased due to the hypoglycemic properties reported for this species. An efficient system for propagation via somatic embryogenesis is reported using petiole segments cultivated on MS medium supplemented with combinations of BA, kinetin and 2,4-D, under light and darkness conditions. Embryogenic callus was formed in most of the treatments; however, somatic embryogenesis was promoted by the presence of light. Clusters of somatic embryos appeared on callus surface after 50 days of culture. The highest number of embryos was produced on 0.45 µM BA and 4.5 µM 2,4-D. Embryogenic calli were maintained on MS medium containing 4.5 µM BA and 0.045 µM 2,4-D. Embryos converted on hormone-free half-strength MS medium with 2 g.L-1 activated charcoal and plantlets were transferred to non-sterile conditions for acclimatization, showing 100% of survival.

scholarly journals Regeneration of Asparagus officinalis L. Through Embryogenic Callus

2017 ◽  
Vol 27 (1) ◽  
pp. 21-31 ◽  
Author(s):  
Mustafa Abul Kalam Azad ◽  
Muhammad Nurul Amin
Keyword(s):  
Somatic Embryogenesis ◽  
Somatic Embryos ◽  
Shoot Proliferation ◽  
Asparagus Officinalis ◽  
Garden Soil ◽  
Ms Medium ◽  
Regeneration System ◽  
Embryo Germination ◽  
Embryogenic Calli

A plant regeneration system was established from hypocotyl explants of in vitro grown seedlings of A. officinalis and in vitro proliferated shoots, respectively through somatic embryogenesis and embryogenic calli. Somatic embryogenesis was significantly influenced by the types of plant growth regulators. Embryogenic calli with somatic embryos developed well in MS supplemented with 2.0 ‐ 4.0 μM BAP and 1.0 ‐ 4.0 μM 22,4‐D, NAA or IBA. The highest frequency (95.3%) of embryogenic calli and 55.2 somatic embryos formation were obtained when the MS was amended with 4.0 μM BAP and 2.0 μM 2,4‐D. The best embryo germination occurred in 1.0 μM BAP supplemented MMS. The highest 97.2% of shoot proliferation was observed in embryogenic calli in MS medium containing 2.0 μM BAP and 1.0 μM IBA. In vitro grown shoots were rooted in MMS with 1.0 ‐ 2.0 μM IBA. Regenerants were transferred to vermicompost and successfully established under an ex vitro environment in garden soil with 80% survival rate.Plant Tissue Cult. & Biotech. 27(1): 21-31, 2017 (June)

PLANT REGENERATION OF KAMCHATIC PLANTAIN (PLANTAGO CAMTSCHATICA LINK) THROUGH CALLUS INDUCTION

2017 ◽  
Vol 19 (3) ◽  
pp. 41-48
Author(s):  
Ay N.V. ◽  
Duy M.V. ◽  
Baatartsogt O. ◽  
Altantsetseg Kh. ◽  
Enkhchimeg V.
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Leaf Explants ◽  
Stage Iv ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Development Stage ◽  
High Survival ◽  
Ms Medium

In vitro seedling offspring of Plantago camtschatica Link was investigated regarding induction of somatic embryogenesis in petiole/leaf explants from shoot tissue and shoot proliferation. The aim of study was to investigate the medium supplemented with suitable concentration of plant growth regulators in order to induce somatic embryogenesis, plant regeneration and shoot multiplication. The results showed that: (i) Petiole/young leaf of immature stem induced the highest ratio of calli induction and compact calli formation on MS medium supplemented with 1 mgL-1 2,4-D and 0.5 mgL-1 BA; (ii) From created calli, somatic embryogenesis could be induced on MS medium supplemented with 1 mgL-1 TDZ or 1 mgL-1 TDZ and 0.5 mgL-1 NAA; (iii) MS medium supplemented with 5-7 mgL-1 BA shown the most effective on shoot development stage; (iv) Rooting of shoot was the best on 1/2 solid MS medium with activated charcoal (2 gL-1), and 0.5-4 mgL-1 NAA; and (v) acclimatization of micropropagated plants could be planted in plastic pots containing a mixture of decayed straw : rice husk ashes, (1:1, v/v), sand : soil (1:1, v/v) or soil, showed a high survival rate and most seedlings grew normally.

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