Optimizing plant regeneration from seed-derived callus cultures of Kentucky bluegrass. The effect of benzyladenine

1995 ◽  
Vol 40 (1) ◽  
pp. 101-103 ◽  
Author(s):  
P. van der Valk ◽  
F. Ruis ◽  
A. M. Tettelaar-Schrier ◽  
C. M. van de Velde
1980 ◽  
Vol 7 (6) ◽  
pp. 635 ◽  
Author(s):  
WR Scowcroft ◽  
PJ Larkin

Mesophyll protoplasts of two genetically distinct genotypes of N. debneyi were cultured with sustained division following a plating efficiency in excess of 50%. Fully fertile mature plants were regenerated from callus cultures derived from protoplasts. Shoots were induced in medium containing 1 mg/l 6-benzylaminopurine and 0.5 mg/I indole acetic acid. The repeatably high efficiency of protoplast culture was used to evaluate the quantitative effects of two drugs, kanamycin and trimethoprim, which effectively inhibited colony formation at concentrations of 100 and 50 �g/ml, respectively. An enhancer of DNA uptake, poly-L-ornithine, had virtually no effect on sustained protoplast division at a concentration of 7.5 �g/ml or less.


1995 ◽  
Vol 51 (1-2) ◽  
pp. 103-113 ◽  
Author(s):  
Xianggan Li ◽  
Denise M. Seliskar ◽  
Jennifer A. Moga ◽  
John L. Gallagher

1993 ◽  
Vol 32 (2) ◽  
pp. 213-218 ◽  
Author(s):  
Archana Giri ◽  
Paramvir Singh Ahuja ◽  
P. V. Ajay Kumar

1997 ◽  
Vol 69 (1-2) ◽  
pp. 85-98 ◽  
Author(s):  
Y. Sahoo ◽  
S.K. Pattnaik ◽  
P.K. Chand

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