Quantitative analysis of depolarization-induced ATP release from mouse brain synaptosomes: External calcium dependent and independent processes

1992 ◽  
Vol 127 (1) ◽  
Author(s):  
JennyL Fiedler ◽  
HarveyB Pollard ◽  
Eduardo Rojas
Keyword(s):  
Quantitative Analysis ◽  
Mouse Brain ◽  
Atp Release ◽  
Calcium Dependent ◽  
Brain Synaptosomes ◽  
External Calcium

The emerging role of calcium-dependent exocytosis in ATP release from nonexcitable cells

2005 ◽  
pp. 21-22 ◽  
Author(s):  
Ryszard Grygorczyk ◽  
Francis Boudreault
Keyword(s):  
Atp Release ◽  
Calcium Dependent

Mouse Brain Synaptosomes Accumulate Copper-67 Efficiently by Two Distinct Processes Independent of Cellular Prion Protein

2005 ◽  
Vol 27 (3) ◽  
pp. 347-354 ◽  
Author(s):  
Armin Giese ◽  
Malte Buchholz ◽  
Jochen Herms ◽  
Hans A. Kretzschmar
Keyword(s):  
Prion Protein ◽  
Mouse Brain ◽  
Cellular Prion Protein ◽  
Brain Synaptosomes

scholarly journals A Quantitative Analysis of the Distribution of CRH Neurons in Whole Mouse Brain

2017 ◽  
Vol 11 ◽  
Author(s):  
Jie Peng ◽  
Ben Long ◽  
Jing Yuan ◽  
Xue Peng ◽  
Hong Ni ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Mouse Brain

Quantitative analysis of the distribution of serotonin-immunoreactive cell bodies in the mouse brain

1988 ◽  
Vol 91 (3) ◽  
pp. 265-270 ◽  
Author(s):  
Kazuhisa Ishimura ◽  
Yoshihiro Takeuchi ◽  
Kozo Fujiwara ◽  
Masashi Tominaga ◽  
Hiroshi Yoshioka ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Mouse Brain ◽  
Immunoreactive Cell

Lipid rafts of purified mouse brain synaptosomes prepared with or without detergent reveal different lipid and protein domains

2003 ◽  
Vol 962 (1-2) ◽  
pp. 144-150 ◽  
Author(s):  
Gunter P Eckert ◽  
Urule Igbavboa ◽  
Walter E Müller ◽  
W.Gibson Wood
Keyword(s):  
Lipid Rafts ◽  
Mouse Brain ◽  
Protein Domains ◽  
Brain Synaptosomes

Calcium-Dependent Plateau Potentials in Rostral Ambiguus Neurons in the Newborn Mouse Brain Stem In Vitro

1997 ◽  
Vol 78 (5) ◽  
pp. 2483-2492 ◽  
Author(s):  
Jens C. Rekling ◽  
Jack L. Feldman
Keyword(s):  
Brain Stem ◽  
Mouse Brain ◽  
Spike Activity ◽  
Nucleus Ambiguus ◽  
Newborn Mouse ◽  
Plateau Potentials ◽  
Calcium Dependent ◽  
Current Pulses ◽  
Voltage Dependent

Rekling, Jens C. and Jack L. Feldman. Calcium-dependent plateau potentials in rostral ambiguus neurons in the newborn mouse brain stem in vitro. J. Neurophysiol. 78: 2483–2492, 1997. The nucleus ambiguus contains vagal and glossopharyngeal motoneurons and preganglionic neurons involved in respiration, swallowing, vocalization, and control of heart beat. Here we show that the rostral compact formation's ambiguus neurons, which control the esophageal phase of swallowing, display calcium-dependent plateau potentials in response to tetanic orthodromic stimulation or current injection. Whole cell recordings were made from visualized neurons in the rostral nucleus ambiguus using a slice preparation from the newborn mouse. Biocytin-labeling revealed dendritic trees with pronounced rostrocaudal orientations confined to the nucleus ambiguus, a morphological profile matching that of vagal motoneurons projecting to the esophagus. Single-stimulus orthodromic activation, using an electrode placed in the dorsomedial slice near the nucleus tractus solitarius, evoked single excitatory postsynaptic potentials (EPSPs) or short trains of EPSPs (500 ms to 1 s). However, tetanic stimulation (5 pulses, 10 Hz) induced voltage-dependent afterdepolarizations or long-lasting plateau potentials (>1 min) with a constant firing pattern. Depolarizing or hyperpolarizing current pulses elicited voltage-dependent afterdepolarizations or plateau potentials lasting a few seconds to several minutes. Constant spike activity accompanied the long-lasting plateau potentials, which ended spontaneously or could be terminated by weak hyperpolarizing current pulses. Current-induced afterdepolarizations and plateau potentials were dependent on extracellularand intracellular Ca2+, as they were blocked completely by extracellular Co2+, Cd2+, or intracellular bis-( o-aminophenoxy)- N,N,N′,N′-tetraacetic acid (BAPTA). Orthodromically induced afterdepolarizations and plateau potentials were blocked by intracellular BAPTA. Afterdepolarizations and plateau potentials were completely blocked by substitution of extracellular Na+ with choline. Afterdepolarizations persisted in tetrodotoxin. We conclude that rostral ambiguus neurons have a Ca2+-activated inward current carried by Na+. Synaptic activation of this conductance may generate prolonged spike activity in these neurons during the esophageal phase of swallowing.

Quantitative analysis of cytokine-induced vascular toxicity and vascular leak in the mouse brain

2009 ◽  
Vol 349 (1-2) ◽  
pp. 45-55 ◽  
Author(s):  
Yetty Y. Irwan ◽  
Yi Feng ◽  
H. Michael Gach ◽  
James T. Symanowski ◽  
John R. McGregor ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Mouse Brain ◽  
Vascular Leak ◽  
Vascular Toxicity

Quantitative analysis of kainate receptor subunits in the mouse brain

2011 ◽  
Vol 71 ◽  
pp. e315-e316
Author(s):  
Izumi Watanabe ◽  
Hirotsugu Azechi ◽  
Kaori Akashi ◽  
Maya Yamazaki ◽  
Manabu Abe ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Mouse Brain ◽  
Kainate Receptor ◽  
Kainate Receptor Subunits

A calcium-independent chloride current activated by hyperpolarization in Xenopus oocytes

1988 ◽  
Vol 233 (1271) ◽  
pp. 191-199 ◽  
Keyword(s):  
Xenopus Laevis ◽  
Xenopus Oocytes ◽  
Chloride Ions ◽  
Chloride Current ◽  
Calcium Dependent ◽  
External Calcium

Hyperpolarization of oocytes of Xenopus laevis usually elicits mainly passive currents. However, when polarized to potentials more negative than about – 100 mV, oocytes obtained from some donors show a relatively well maintained current that is carried mainly by chloride ions. This response does not depend upon external calcium, and is thus clearly different from the calcium-dependent transient chloride current previously described.

Sign in / Sign up

Export Citation Format

Share Document