ATP-sensitive K+ channel opener acts as a potent Cl? channel inhibitor in vascular smooth muscle cells

1994 ◽  
Vol 137 (1) ◽  
Author(s):  
K.O. Holevinsky ◽  
Z. Fan ◽  
M. Frame ◽  
J.C. Makielski ◽  
V. Groppi ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells ◽  
K Channel ◽  
Cl Channel ◽  
Channel Opener ◽  
K Channel Opener

scholarly journals Principal role of adenylyl cyclase 6 in K+ channel regulation and vasodilator signalling in vascular smooth muscle cells

2011 ◽  
Vol 91 (4) ◽  
pp. 694-702 ◽  
Author(s):  
Carl P. Nelson ◽  
Richard D. Rainbow ◽  
Jennifer L. Brignell ◽  
Matthew D. Perry ◽  
Jonathon M. Willets ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Adenylyl Cyclase ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells ◽  
K Channel ◽  
Principal Role ◽  
Channel Regulation

scholarly journals 108 THE FUNCTIONAL ROLE OF CA2+-ACTIVATED CL- CHANNEL CANDIDATES IN VASCULAR SMOOTH MUSCLE CELLS

2012 ◽  
Vol 30 ◽  
pp. e33
Author(s):  
Vibeke Secher Dam ◽  
Torbjarn Bragger ◽  
Donna Badtkjer ◽  
Christian Aalkjalr ◽  
Vladimir Matchkov
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Functional Role ◽  
Muscle Cells ◽  
Cl Channel

MCI-154 activates the Ca2+-activated K+ channel of vascular smooth muscle cells

Life Sciences ◽  
1995 ◽  
Vol 56 (15) ◽  
pp. PL291-PL298 ◽  
Author(s):  
Hirokazu Miyoshi ◽  
Yutaka Nakaya ◽  
Ken Saito ◽  
Fumiko Kishi ◽  
Masahiro Takakura ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells ◽  
K Channel

scholarly journals Vasodilatory Effect of Phellinus linteus Extract in Rat Mesenteric Arteries

Molecules ◽  
2020 ◽  
Vol 25 (14) ◽  
pp. 3160
Author(s):  
Youngin Kwon ◽  
Chae Eun Haam ◽  
Seonhee Byeon ◽  
Soo Jung Choi ◽  
Dong-Hoon Shin ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Channel Blocker ◽  
Muscle Cells ◽  
Mesenteric Arteries ◽  
K Channel ◽  
Phellinus Linteus ◽  
Ikca Channel

Phellinus linteus is a well-known medicinal mushroom that is widely used in Asian countries. In several experimental models, Phellinus linteus extracts were reported to have various biological effects, including anti-inflammatory, anti-cancer, hepatoprotective, anti-diabetic, neuroprotective, and anti-angiogenic activity. In the present study, several bioactive compounds, including palmitic acid ethyl ester and linoleic acid, were identified in Phellinus linteus. The intermediate-conductance calcium-activated potassium channel (IKCa) plays an important role in the regulation of the vascular smooth muscle cells’ (VSMCs) contraction and relaxation. The activation of the IKCa channel causes the hyperpolarization and relaxation of VSMCs. To examine whether Phellinus linteus extract causes vasodilation in the mesenteric arteries of rats, we measured the isometric tension using a wire myograph. After the arteries were pre-contracted with U46619 (a thromboxane analogue, 1 µM), Phellinus linteus extract was administered. The Phellinus linteus extract induced vasodilation in a dose-dependent manner, which was independent of the endothelium. To further investigate the mechanism, we used the non-selective K+ channel blocker tetraethylammonium (TEA). TEA significantly abolished Phellinus linteus extract-induced vasodilation. Thus, we tested three different types of K+ channel blockers: iberiotoxin (BKca channel blocker), apamin (SKca channel blocker), and charybdotoxin (IKca channel blocker). Charybdotoxin significantly inhibited Phellinus linteus extract-induced relaxation, while there was no effect from apamin and iberiotoxin. Membrane potential was measured using the voltage-sensitive dye bis-(1,3-dibutylbarbituric acid)-trimethine oxonol (DiBAC4(3)) in the primary isolated vascular smooth muscle cells (VSMCs). We found that the Phellinus linteus extract induced hyperpolarization of VSMCs, which is associated with a reduced phosphorylation level of 20 KDa myosin light chain (MLC20).

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