Simultaneous detection of neuropeptides and messenger RNA in the magnocellular hypothalamo-neurohypophysial system by a combination of non-radioactive in situ hybridization histochemistry and immunohistochemistry

1994 ◽  
Vol 102 (6) ◽  
pp. 415-423 ◽  
Author(s):  
P. J. Larsen ◽  
J. D. Mikkelsen
1988 ◽  
Vol 537 (1 The Mesocorti) ◽  
pp. 484-487 ◽  
Author(s):  
FRANK BALDING ◽  
ARIEL Y. DEUTCH ◽  
ROBERT H. ROTH ◽  
MICHAEL E. LEWIS

FEBS Letters ◽  
1989 ◽  
Vol 255 (2) ◽  
pp. 335-339 ◽  
Author(s):  
A. Najlerahim ◽  
A.J.L. Barton ◽  
P.J. Harrison ◽  
J. Heffernan ◽  
R.C.A. Pearson

1988 ◽  
Vol 118 (2) ◽  
pp. 205-NP ◽  
Author(s):  
A. Levy ◽  
S. L. Lightman

ABSTRACT The temporal effect of orally administered bromocriptine on pro-opiomelanocortin (POMC) and prolactin gene expression in male Sprague–Dawley rats was examined using in-situ hybridization histochemistry. Messenger RNA in the anterior and intermediate lobes could be clearly delineated in each section. Administration of bromocriptine resulted in a reduction in hybridization of 35S-labelled cDNA probe to prolactin mRNA from 0·69 × 1012 to 0·29 × 1012 copies bound/g after 150 h. POMC mRNA in the anterior lobe remained unchanged with 0·08 × 1012 copies of probe bound/g for the duration of the experiment, while in the intermediate lobe it decreased from 2·44 × 1012 to 0·44 × 1012 copies of probe bound/g at 150 h. The rate of reduction in intermediate lobe POMC mRNA was similar to that of prolactin mRNA for the first 24 h but was subsequently more rapid and more profound, falling to 20% of the control value at 84 h and to 18% at 150 h. J. Endocr. (1988) 118, 205–210


Neuroscience ◽  
1995 ◽  
Vol 64 (4) ◽  
pp. 1105-1111 ◽  
Author(s):  
R.P. Ward ◽  
M.W. Hamblin ◽  
J.E. Lachowicz ◽  
B.J. Hoffman ◽  
D.R. Sibley ◽  
...  

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