Development and behavior of synaptonemal complexes in human spermatocytes by light and electron microscopy

1984 ◽  
Vol 68 (2) ◽  
pp. 142-147 ◽  
Author(s):  
F. Vidal ◽  
J. Navarro ◽  
C. Templado ◽  
S. Marina ◽  
J. Egozcue
Keyword(s):  
Electron Microscopy ◽  
Light And Electron Microscopy ◽  
Synaptonemal Complexes ◽  
And Behavior

A method for the sequential study of synaptonemal complexes by light and electron microscopy

1981 ◽  
Vol 59 (4) ◽  
pp. 419-421 ◽  
Author(s):  
J. Navarro ◽  
F. Vidal ◽  
M. Guitart ◽  
J. Egozcue
Keyword(s):  
Electron Microscopy ◽  
Light And Electron Microscopy ◽  
Synaptonemal Complexes ◽  
Sequential Study

scholarly journals AN ULTRASTRUCTURAL STUDY OF PLANT SPERMATOGENESIS

1968 ◽  
Vol 37 (2) ◽  
pp. 370-393 ◽  
Author(s):  
F. R. Turner
Keyword(s):  
Electron Microscopy ◽  
Golgi Apparatus ◽  
Ultrastructural Study ◽  
Light And Electron Microscopy ◽  
Mature Sperm ◽  
Basal Bodies ◽  
Animal Cells ◽  
Early Spermatid ◽  
And Behavior ◽  
Cytoskeletal System

Spermatogenesis in the charophyte Nitella has been followed in antheridia prepared for light and electron microscopy. The antheridial filament cells contain paired centrioles which are similar in structure and behavior to the centrioles of animal cells. In the early spermatid, the centrioles undergo an initial elongation at their distal ends and become joined by a spindle-shaped fibrous connection. At the same time, their proximal ends are closely associated with the development of a layer of juxtaposed microtubules which will form the microtubular sheath. The architectural arrangement of these microtubules suggests that they constitute a cytoskeletal system, forming a framework along which the mitochondria and plastids become aligned and along which the nucleus undergoes extensive elongation and differentiation. The microtubular sheath persists in the mature sperm. During mid-spermatid stages, the centrioles give rise to the flagella and concomitantly undergo differentiation to become the basal bodies. The Golgi apparatus goes through a period of intensive activity during mid-spermatid stages, then decreases in organization until it can no longer be detected in the late spermatid. An attempt is made to compare similarities between plant and animal spermiogenesis.

A TECHNIQUE FOR LIGHT AND ELECTRON MICROSCOPY OF THE SYNAPTONEMAL COMPLEX OF THE MOUSE OOCYTE

1982 ◽  
Vol 24 (6) ◽  
pp. 675-680 ◽  
Author(s):  
Weng Kong Sung ◽  
Georgiana Jagiello
Keyword(s):  
Electron Microscopy ◽  
Synaptonemal Complex ◽  
Microscopic Examination ◽  
Light And Electron Microscopy ◽  
Electron Microscopic Examination ◽  
Mouse Oocyte ◽  
Electron Microscopic ◽  
Synaptonemal Complexes

A method is described for obtaining synaptonemal complex preparations from mouse pachytene oocytes for light and electron microscopic examination. A karyotype based on the whole complement of synaptonemal complexes of a pachytene oocyte as visualized by electron microscopy is presented.

Light and electron microscope observations of a meiotic mutant of Neurospora crassa

1978 ◽  
Vol 56 (21) ◽  
pp. 2694-2706 ◽  
Author(s):  
B.C. Lu ◽  
Donna R. Galeazzi
Keyword(s):  
Electron Microscopy ◽  
Electron Microscope ◽  
Genetic Analysis ◽  
Neurospora Crassa ◽  
Synaptonemal Complex ◽  
Chromosome Pairing ◽  
Light And Electron Microscopy ◽  
Homologous Chromosomes ◽  
Synaptonemal Complexes ◽  
Nuclear Separation

Light and electron microscopy have revealed that the meiotic-1 (mei-1) mutant of Neurospora crassa is defective in chromosome pairing (asynaptic) although plenty of axial components of the synaptonemal complex are produced and occasional tripartite synaptonemal complexes can be formed. The mei-1 mutant is most probably defective in bringing the homologous chromosomes together for pairing and for assembly of the synaptonemal complex. The mei-1 mutant is also defective in nuclear separation which leads to a four-poled spindle at the subsequent division. The lack of chromosome pairing, the incomplete assembly of the synaptonemal complex, and the four-poled spindles account for absence of recombination and for the nondisjunction found in genetic analysis.

Sequential study of synaptonemal complexes in mouse spermatocytes by light and electron microscopy

Genetica ◽  
1985 ◽  
Vol 67 (1) ◽  
pp. 21-30 ◽  
Author(s):  
M. Guitart ◽  
M. D. Coll ◽  
M. Ponsà ◽  
J. Egozcue
Keyword(s):  
Electron Microscopy ◽  
Light And Electron Microscopy ◽  
Synaptonemal Complexes ◽  
Sequential Study

The ultrastructure of meiosis in Plasmodiophora brassicae (Plasmodiophorales)

1979 ◽  
Vol 57 (22) ◽  
pp. 2509-2518 ◽  
Author(s):  
Robert C. Garber ◽  
James R. Aist
Keyword(s):  
Electron Microscopy ◽  
Vegetative Growth ◽  
Plasmodiophora Brassicae ◽  
Light And Electron Microscopy ◽  
Synaptonemal Complexes ◽  
Prophase I ◽  
Meiosis I

Meiosis was examined in plasmodia of the protist Plasmodiophora brassicae within artificially inoculated cabbage roots, using light and electron microscopy. Meiotic nuclear divisions occur following the cessation of vegetative growth of the Plasmodium. Synaptonemal complexes develop in nuclei of the “akaryote stage,” which represents prophase I. Meiosis I and II take place concurrently with cleavage of the Plasmodium into resting sporangia. Previous reports of synaptonemal complexes and sporangiogenic meiosis in the Plasmodiophorales are thus corroborated. Centrioles are paired and bipolar until the end of meiosis I; then they separate and migrate to opposite poles, without replicating, between prophase II and metaphase II. Centrioles elongate considerably between prophase I and the end of meiosis II, then appear to disintegrate as uninucleate resting sporangia are formed and are absent from mature sporangia.

The Morphology of Vacuolated Cells in the Liver Following Administration of Vitamin A.

1973 ◽  
Vol 31 ◽  
pp. 408-409
Author(s):  
Odell T. Minick ◽  
Hidejiro Yokoo ◽  
Fawzia Batti
Keyword(s):  
Electron Microscopy ◽  
Body Weight ◽  
Vitamin A ◽  
Light And Electron Microscopy ◽  
Liver Cells ◽  
Prominent Feature ◽  
Vascular Space ◽  
Vacuolated Cell ◽  
Vacuolated Cells ◽  
Vacuolated Cytoplasm

Vacuolated cells in the liver of young rats were studied by light and electron microscopy following the administration of vitamin A (200 units per gram of body weight). Their characteristics were compared with similar cells found in untreated animals.In rats given vitamin A, cells with vacuolated cytoplasm were a prominent feature. These cells were found mostly in a perisinusoidal location, although some appeared to be in between liver cells (Fig. 1). Electron microscopy confirmed their location in Disse's space adjacent to the sinusoid and in recesses between liver cells. Some appeared to be bordering the lumen of the sinusoid, but careful observation usually revealed a tenuous endothelial process separating the vacuolated cell from the vascular space. In appropriate sections, fenestrations in the thin endothelial processes were noted (Fig. 2, arrow).

Control of Autogenous Vein Grafts Prior to Reimplantation, By Electron Microscopy

1972 ◽  
Vol 30 ◽  
pp. 106-107
Author(s):  
John H. L. Watson ◽  
John L. Swedo ◽  
M. Vrandecic
Keyword(s):  
Electron Microscopy ◽  
Light And Electron Microscopy ◽  
Physiological Saline ◽  
Experimental Conditions ◽  
Vein Grafts ◽  
Arterial Blood ◽  
Saphenous Veins ◽  
Autogenous Vein ◽  
Control Of Parameters ◽  
Post Implantation

The ambient temperature and the nature of the storage fluids may well have significant effects upon the post-implantation behavior of venus autografts. A first step in the investigation of such effects is reported here. Experimental conditions have been set which approximate actual operating room procedures. Saphenous veins from dogs have been used as models in the experiments. After removal from the dogs the veins were kept for two hours under four different experimental conditions, viz at either 4°C or 23°C in either physiological saline or whole canine arterial blood. At the end of the two hours they were prepared for light and electron microscopy. Since no obvious changes or damage could be seen in the veins by light microscopy, even with the advantage of tissue specific stains, it was essential that the control of parameters for successful grafts be set by electron microscopy.

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