Isolation and characterization of the SUD1 gene, which encodes a global repressor of core promoter activity in Saccharomyces cerevisiae

1993 ◽  
Vol 241-241 (5-6) ◽  
pp. 616-626 ◽  
Author(s):  
Ichiro Yamashita
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Promoter Activity ◽  
Core Promoter ◽  
Isolation And Characterization ◽  
Core Promoter Activity

scholarly journals Isolation and characterization of pre-mRNA splicing mutants of Saccharomyces cerevisiae.

1989 ◽  
Vol 3 (8) ◽  
pp. 1206-1216 ◽  
Author(s):  
U Vijayraghavan ◽  
M Company ◽  
J Abelson
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Mrna Splicing ◽  
Isolation And Characterization

Mutations at the Saccharomyces cerevisiae SUP4 tRNA Tyr Locus: Isolation, Genetic Fine-Structure Mapping, and Correlation with Physical Structure

1982 ◽  
Vol 2 (12) ◽  
pp. 1501-1513
Author(s):  
Janet Kurjan ◽  
Benjamin D. Hall
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Fine Structure ◽  
Physical Structure ◽  
Mapping Technique ◽  
Structure Mapping ◽  
Isolation And Characterization ◽  
Qualitative Estimate ◽  
Genetic Fine Structure ◽  
Entire Gene

The SUP4 tRNA Tyr locus in Saccharomyces cerevisiae has been studied by the isolation and characterization of mutations at the SUP4 gene which result in the loss of suppressor function. Most of the mutations act as single-site mutations, whereas about a third of the mutations are deletions of the entire gene. Two meiotic fine-structure maps of the gene were made. The first mapping technique placed 10 mutations plus the sup4 + anticodon on a map by a measurement of levels of recombination between pairs of mutations. The second map utilized a more qualitative estimate of recombination frequency, allowing 69 mutations and the sup4 + anticodon to be mapped. The maps were compared with the physical structure of the gene for the 34 mutations whose nucleotide alteration has been determined by DNA sequencing (Koski et al., Cell 22 :415-425, 1980; Kurjan et al., Cell 20 :701-709, 1980). Both maps show a good correlation with the physical structure of the gene, even though certain properties of genetic fine-structure maps, such as marker effects and “map expansion,” were seen.

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