Somatic hybrid plants produced by electrofusion between Solanum melongena L. and Solanum torvum Sw

1989 ◽  
Vol 77 (1) ◽  
pp. 1-6 ◽  
Author(s):  
D. Sihachakr ◽  
R. Haicour ◽  
M. -H. Chaput ◽  
E. Barrientos ◽  
G. Ducreux ◽  
...  



Plant Science ◽  
1988 ◽  
Vol 57 (3) ◽  
pp. 215-223 ◽  
Author(s):  
Darasinh Sihachakr ◽  
Robert Haicour ◽  
Isabelle Serraf ◽  
Edilberto Barrientos ◽  
Catherine Herbreteau ◽  
...  


1995 ◽  
Vol 14 (10) ◽  
pp. 652-656 ◽  
Author(s):  
K. B. Liu ◽  
Y.-M. Li ◽  
K. C. Sink


Planta ◽  
2007 ◽  
Vol 226 (4) ◽  
pp. 961-969 ◽  
Author(s):  
Lorena Borgato ◽  
Clara Conicella ◽  
Federica Pisani ◽  
Antonella Furini


HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 840B-840
Author(s):  
Vladimir M. Samoylov ◽  
Kenneth C. Sink

Asymmetric somatic hybrids were obtained by PEG/DMSO fusion of protoplasts of a kanamycin-resistant (KmR+) interspecific tomato hybrid L. esculentum × L. pennellii with protoplasts of S. melongena eggplant. Elimination of tomato chromosomes was directed by application of 100, 250, 500, 750, and 1000 Gy of γ-rays to the donor protoplasts. The hybrid nature of selected KmR+ calli was confirmed by RAPDs, PCR amplification of the NptII gene, and Southern hybridization. Flow cytometry revealed that asymmetric hybrid plants were regenerated only from selected somatic hybrid calli that had a ploidy level close to 4n. The amount of donor DNA in three somatic hybrid plants was quantified by dot-blot hybridization with tomato species-specific probes, and was found to be 5% to 7%. Therefore, presence of four to five tomato chromosomes in asymmetric hybrid plants has been calculated. Detection of tomato-specific chromosomes in hybrid plants by RFLP analysis will be presented.



Pathogens ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 470
Author(s):  
Min Zhang ◽  
Hongyuan Zhang ◽  
Jie Tan ◽  
Shuping Huang ◽  
Xia Chen ◽  
...  

Eggplant (Solanum melongena L.), which belongs to the Solanaceae family, is an important vegetable crop. However, its production is severely threatened by root-knot nematodes (RKNs) in many countries. Solanum torvum, a wild relative of eggplant, is employed worldwide as rootstock for eggplant cultivation due to its resistance to soil-borne diseases such as RKNs. In this study, to identify the RKN defense mechanisms, the transcriptomic profiles of eggplant and Solanum torvum were compared. A total of 5360 differentially expressed genes (DEGs) were identified for the response to RKN infection. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis showed that these DEGs are mainly involved in the processes of response to stimulus, protein phosphorylation, hormone signal transduction, and plant-pathogen interaction pathways. Many phytohormone-related genes and transcription factors (MYB, WRKY, and NAC) were differentially expressed at the four time points (ck, 7, 14, and 28 days post-infection). The abscisic acid signaling pathway might be involved in plant-nematode interactions. qRT-PCR validated the expression levels of some of the DEGs in eggplant. These findings demonstrate the nematode-induced expression profiles and provide some insights into the nematode resistance mechanism in eggplant.







2015 ◽  
Vol 16 (12) ◽  
pp. 7608-7626 ◽  
Author(s):  
Xu Yang ◽  
Cao Deng ◽  
Yu Zhang ◽  
Yufu Cheng ◽  
Qiuyue Huo ◽  
...  


2002 ◽  
Vol 71 (5) ◽  
pp. 623-631 ◽  
Author(s):  
Masahito Shimonaka ◽  
Takashi Hosoki ◽  
Motonori Tomita ◽  
Yoshimasa Yasumuro


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