Electrofusion for the production of somatic hybrid plants of Solanum melongena L. and Solanum khasianum C.B. Clark

Asymmetric somatic hybrids were obtained by PEG/DMSO fusion of protoplasts of a kanamycin-resistant (KmR+) interspecific tomato hybrid L. esculentum × L. pennellii with protoplasts of S. melongena eggplant. Elimination of tomato chromosomes was directed by application of 100, 250, 500, 750, and 1000 Gy of γ-rays to the donor protoplasts. The hybrid nature of selected KmR+ calli was confirmed by RAPDs, PCR amplification of the NptII gene, and Southern hybridization. Flow cytometry revealed that asymmetric hybrid plants were regenerated only from selected somatic hybrid calli that had a ploidy level close to 4n. The amount of donor DNA in three somatic hybrid plants was quantified by dot-blot hybridization with tomato species-specific probes, and was found to be 5% to 7%. Therefore, presence of four to five tomato chromosomes in asymmetric hybrid plants has been calculated. Detection of tomato-specific chromosomes in hybrid plants by RFLP analysis will be presented.

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A cytogenetic and phenotypic characterization of somatic hybrid plants obtained after fusion of two different dihaploid clones of potato (Solatium tuberosum L.)

Theoretical and Applied Genetics ◽

10.1007/bf00222329 ◽

1992 ◽

Vol 85 (4) ◽

pp. 470-479 ◽

Cited By ~ 15

Author(s):

S. Waara ◽

L. Pijnacker ◽

M. A. Ferwerda ◽

A. Wallin ◽

T. Eriksson

Keyword(s):

Somatic Hybrid ◽

Phenotypic Characterization ◽

Hybrid Plants ◽

Solatium Tuberosum

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Molecular analysis of single copy and repetitive genes on chromosome 2 in intergeneric tomato somatic hybrid plants

Plant Molecular Biology ◽

10.1007/bf00015666 ◽

1988 ◽

Vol 11 (2) ◽

pp. 139-145 ◽

Cited By ~ 9

Author(s):

Patrick P. Moore ◽

Kenneth C. Sink

Keyword(s):

Molecular Analysis ◽

Somatic Hybrid ◽

Single Copy ◽

Chromosome 2 ◽

Hybrid Plants

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Production of Somatic Hybrid Plants between Japanese Bunching Onion(Allium fistulosum L.) and Bulb Onion(A. cepa L.) via Electrofusion.

Engei Gakkai zasshi ◽

10.2503/jjshs.71.623 ◽

2002 ◽

Vol 71 (5) ◽

pp. 623-631 ◽

Cited By ~ 4

Author(s):

Masahito Shimonaka ◽

Takashi Hosoki ◽

Motonori Tomita ◽

Yoshimasa Yasumuro

Keyword(s):

Somatic Hybrid ◽

Allium Fistulosum ◽

Bulb Onion ◽

Hybrid Plants ◽

Japanese Bunching Onion ◽

Bunching Onion

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Selection of Somatic Hybrid Plants in Nicotiana Through Fluorescence-Activated Sorting of Protoplasts

Nature Biotechnology ◽

10.1038/nbt0985-811 ◽

1985 ◽

Vol 3 (9) ◽

pp. 811-816 ◽

Cited By ~ 39

Author(s):

Claudio L. Afonso ◽

Kristi R. Harkins ◽

Mary A. Thomas-Compton ◽

Ann E. Krejci ◽

David W. Galbraith

Keyword(s):

Somatic Hybrid ◽

Hybrid Plants ◽

Selection Of

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Caipira sweet orange + Rangpur lime: a somatic hybrid with potential for use as rootstock in the Brazilian citrus industry

Genetics and Molecular Biology ◽

10.1590/s1415-47572000000300026 ◽

2000 ◽

Vol 23 (3) ◽

pp. 661-665 ◽

Cited By ~ 17

Author(s):

Fernanda Januzzi Mendes-da-Glória ◽

Francisco de Assis Alves Mourão Filho ◽

Luis Eduardo Aranha Camargo ◽

Beatriz Madalena Januzzi Mendes

Keyword(s):

Somatic Hybrid ◽

Leaf Morphology ◽

Somatic Hybrids ◽

Sweet Orange ◽

Southeastern Brazil ◽

Citrus Industry ◽

Rangpur Lime ◽

Rapd Profile ◽

Hybrid Plants ◽

Regenerated Plants

Protoplast culture following polyethylene glycol-induced fusion resulted in the regeneration of somatic hybrid plants between Caipira sweet orange (Citrus sinensis L. Osbeck) and Rangpur lime (C. limonia L. Osbeck). The plants were confirmed as somatic hybrids by leaf morphology, chromosome number and RAPD profile. All regenerated plants were tetraploid (2n = 4x = 36), with intermediate leaf morphology and complementary RAPD banding profile of both parents. This combination may be useful as a rootstock for the citrus industry in Southeastern Brazil since this somatic hybrid could combine the drought tolerance and vigor of Rangpur lime with the blight tolerance of Caipira sweet orange.

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