Histochemical demonstration of enzymes in rat liver during post-natal development

1970 ◽  
Vol 23 (1) ◽  
pp. 71-81 ◽  
Author(s):  
J. Koudstaal ◽  
M. J. Hardonk
Keyword(s):  
Rat Liver ◽  
Histochemical Demonstration

scholarly journals Freeze substituted tissue in 5'-nucleotidase histochemistry. Comparative histochemical and biochemical investigations.

1979 ◽  
Vol 27 (12) ◽  
pp. 1582-1587 ◽  
Author(s):  
K Klaushofer ◽  
H von Mayersbach
Keyword(s):  
Enzyme Activity ◽  
Sensitivity And Specificity ◽  
Rat Liver ◽  
Freeze Substitution ◽  
High Sensitivity ◽  
Histochemical Demonstration ◽  
Frozen Sections ◽  
Enzyme Localization ◽  
Fresh Frozen ◽  
Preparation Techniques

The suitability of freeze-substitution in n-butanol and paraffin embedding of tissues for the histochemical demonstration of 5'-nucleotidase was investigated and compared with commonly used preparation techniques, such as fresh frozen sections and cryostate sections of cold formalin and glutaraldehyde-fixed rat liver. The influences of each step of the preparation techniques on the enzyme activity were controlled by a quantitative radiochemical assay. Freeze substitution was revealed to be superior to the commonly used preparation techniques with respect to: 1) high sensitivity and specificity of the histochemical 5'-nucleotidase reaction (this is based on the fact that incubation media with very low lead concentrations (0,6 mM/1) can be used); 2) excellent morphological appearance of the tissues showing cytological details of enzyme localization; 3) unlimited storage of the tissue materials and ease of sectioning.

Biochemical and Histochemical Demonstration of Transferrin Receptors on Parenchymal and Non-Parenchymal Cells of Rat Liver

1985 ◽  
Vol 68 (s11) ◽  
pp. 63P-63P
Author(s):  
W Vogel ◽  
SP Young ◽  
A Bomford ◽  
Roger Williams
Keyword(s):  
Rat Liver ◽  
Histochemical Demonstration ◽  
Transferrin Receptors ◽  
Parenchymal Cells

Histochemical demonstration of copper in LEC rat liver

1993 ◽  
Vol 100 (4) ◽  
pp. 249-256 ◽  
Author(s):  
Y. Fujii ◽  
K. Shimizu ◽  
M. Satoh ◽  
M. Fujita ◽  
Y. Fujioka ◽  
...  
Keyword(s):  
Rat Liver ◽  
Histochemical Demonstration

The histochemical demonstration of aniline hydroxylase activity in rat liver

1971 ◽  
Vol 3 (2) ◽  
pp. 107-116 ◽  
Author(s):  
S. Gangolli ◽  
M. Wright
Keyword(s):  
Rat Liver ◽  
Histochemical Demonstration ◽  
Hydroxylase Activity ◽  
Aniline Hydroxylase

scholarly journals CYTOPHOTOMETRIC QUANTITATION OF GLUCOSE 6-PHOSPHATASE ACTIVITY IN RAT LIVER

1969 ◽  
Vol 17 (2) ◽  
pp. 107-109 ◽  
Author(s):  
D. E. ABRAHAMSON ◽  
J. L. RIGATUSO ◽  
A. LAZAROW
Keyword(s):  
Phosphatase Activity ◽  
Rat Liver ◽  
Optical Density ◽  
Incubation Time ◽  
Specific Inhibitor ◽  
Histochemical Demonstration ◽  
Precipitation Method ◽  
Normal Tissues ◽  
Fresh Frozen ◽  
Normal Rat

The Wachstein-Meisel lead sulfide precipitation method was used for the histochemical demonstration of glucose 6-phosphatase activity in fresh-frozen normal rat liver sections. Transmittances, microspectrophotometrically determined over cytoplasmic areas of stained sections, were used to calculate the optical density of the stain. In spite of the fact that no absorption maximum was found, the spectrum of glucose 6-phosphatase stain showed proportional increases with increased incubation time. The extinction at a fixed wavelength was shown to be a linear function of incubation time. Studies in which normal tissues were incubated in buffer-substrate and then removed and reincubated for an additional period of time in buffer-substrate containing a specific inhibitor of glucose 6-phosphatase indicate that the increase in optical density of the stain was a result of glucose 6-phosphatase activity.

scholarly journals HISTOCHEMICAL DEMONSTRATION OF INCREASED ACTIVITY OF γ-GLUTAMYL TRANSPEPTIDASE IN RAT LIVER DURING HEPATOCARCINOGENESIS

1976 ◽  
Vol 9 (2) ◽  
pp. 168-179 ◽  
Author(s):  
MASAOKI HARADA ◽  
KAZUHIKO OKABE ◽  
KAZUTOSHI SHIBATA ◽  
HIROTAKE MASUDA ◽  
KEIKO MIYATA ◽  
...  
Keyword(s):  
Rat Liver ◽  
Histochemical Demonstration ◽  
Glutamyl Transpeptidase ◽  
Increased Activity
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