Genetic analysis of DNA fingerprints and virulences in Erysiphe graminis f.sp. hordei

1994 ◽  
Vol 26 (2) ◽  
pp. 172-178 ◽  
Author(s):  
James K. M. Brown ◽  
Craig G. Simpson
Keyword(s):  
Genetic Analysis ◽  
Erysiphe Graminis ◽  
Dna Fingerprints

scholarly journals Genetic Analysis of Inonotus Obliquus Strains by RAPD

2007 ◽  
Vol 26 (3) ◽  
pp. 201-205
Author(s):  
Yan-Qiu Chen ◽  
Xiao-Fan Guo ◽  
Chang-Tian Li ◽  
Yu Li
Keyword(s):  
Genetic Diversity ◽  
Genetic Variability ◽  
Genetic Analysis ◽  
Genetic Distances ◽  
Dna Fingerprints ◽  
Inonotus Obliquus ◽  
Medicinal Fungus ◽  
Different Strains ◽  
Rapd Fragment ◽  
Intraspecific Difference

Genetic Analysis ofInonotus ObliquusStrains by RAPDRAPD profiling of eightInonotus obliquusstrains isolated from sclerotia collected from different areas of China was conducted to determine the genetic variability within this important medicinal fungus and to better define relationships between the genotype and geographical origins of isolation. Twelve 10-mer primers generated a total of 167 stable and reproducible DNA fragments, of which 101 (60.5%) were polymorphic. DNA fingerprints revealed genetic diversity among the strains tested, but there was the little intraspecific difference between the fingerprints of individual strains. A phenogram constructed based on UPGMA analysis of genetic distances calculated from RAPD fragment data identified three distinct groupings: (1) BCX01 and BCX02, (2) JL01, JL02, JL03, JL04 and JL05, (3) HLJ01. Our data confirm that the genetic variability among different strains may be a useful ancillary tool for identifyingl. obliquussclerotia of different geographical origins.

Genetic analysis of interactions between Aegilops species and formae speciales of Erysiphe graminis

1995 ◽  
Vol 70 (1) ◽  
pp. 127-134
Author(s):  
Yukio TOSA ◽  
Keishi MATSUMURA ◽  
Takehiko HOSAKA
Keyword(s):  
Genetic Analysis ◽  
Erysiphe Graminis ◽  
Aegilops Species ◽  
Formae Speciales

Genetic analysis of the avirulence of wheatgrass powdery mildew fungus on common wheat

Genome ◽  
1989 ◽  
Vol 32 (5) ◽  
pp. 913-917 ◽  
Author(s):  
Y. Tosa
Keyword(s):  
Triticum Aestivum ◽  
Powdery Mildew ◽  
Genetic Analysis ◽  
Common Wheat ◽  
Erysiphe Graminis ◽  
Wheat Cultivars ◽  
Avirulence Genes ◽  
Powdery Mildew Fungus ◽  
Major Genes ◽  
Formae Speciales

F1 hybrid cultures between Erysiphe graminis f.sp. agropyri (wheatgrass mildew fungus) and E. graminis f.sp. tritici (wheat mildew fungus) were produced by using a common host of the two formae spéciales. When three common wheat cultivars, Triticum aestivum cv. Norin 4, T. aestivum cv. Norin 10, and T. compactum cv. No. 44, were inoculated with a population of F1 cultures, avirulent and virulent cultures segregated in a 3:1 ratio. This indicated that two major genes are involved in the avirulence of E. graminis f.sp. agropyri, Ak-1, on each of the three cultivars. Further analyses revealed that the three pairs of avirulence genes have one gene in common. On T. aestivum cv. Shin-chunaga, T. aestivum cv. Norin 26, and a strain of T. macha, the F1 population segregated in the same pattern as on T. aestivum cv. Norin 4, indicating that the same pair of avirulence genes is operating on these four cultivars. On T. aestivum cv. Red Egyptian the distribution of F1 phenotypes was continuous, suggesting that no major genes are involved in the avirulence of Ak-1 on this cultivar.Key words: powdery mildew, Erysiphe graminis, avirulence, wheat, wheatgrass.

Application of DNA fingerprints for identification and genetic analysis of Carica papaya and other Carica species

Euphytica ◽  
1992 ◽  
Vol 62 (2) ◽  
pp. 119-126 ◽  
Author(s):  
Dror Sharon ◽  
Jossi Hillel ◽  
Alexander Vainstein ◽  
Uri Lavi
Keyword(s):  
Genetic Analysis ◽  
Carica Papaya ◽  
Dna Fingerprints

scholarly journals Application of DNA Fingerprints for Identification and Genetic Analyses of Mango (Mangifera indica) Genotypes

1995 ◽  
Vol 120 (2) ◽  
pp. 259-264 ◽  
Author(s):  
A. Adato ◽  
D. Sharon ◽  
U. Lavi ◽  
J. Hillel ◽  
S. Gazit
Keyword(s):  
Genetic Analysis ◽  
Genomic Dna ◽  
Genetic Relatedness ◽  
Mangifera Indica ◽  
Genetic Distances ◽  
Dna Fingerprint ◽  
Dna Fingerprints ◽  
Genetic Analyses ◽  
Young Leaves ◽  
Relatedness Analysis

DNA fingerprint information was used for identification of mango (Mangifera indica L.) cultivars for genetic relatedness analysis of20 mango cultivars and for genetic analysis of a family structure. Genomic DNA was extracted from young leaves, digested with Hind III or Dra I, and hybridized with 10 different DNA probes. Jeffreys' minisatellite probe 33.6 was the most useful, resulting in well-resolved bands representing highly polymorphic loci. Specific patterns were obtained for each cultivar. The probability of obtaining a similar pattern for two different cultivars was 9.4 × 10-6. Based on DNA fingerprint information, genetic distances between 20 mango cultivars were evaluated and an evolutionary tree was established. Analysis of DNA fingerprint band patterns of 12 progeny resulting from a cross between `Tommy Atkins' and `Keitt' mango revealed neither linked nor allelic bands. Application of the reported results for identification, genetic analyses, and mango breeding is discussed.

scholarly journals Application of DNA Fingerprints for Identification and Genetic Analysis of Avocado

1991 ◽  
Vol 116 (6) ◽  
pp. 1078-1081 ◽  
Author(s):  
U. Lavi ◽  
J. Hillel ◽  
A. Vainstein ◽  
E. Lahav ◽  
D. Sharon
Keyword(s):  
Genetic Analysis ◽  
Family Structure ◽  
Genomic Dna ◽  
Persea Americana ◽  
Dna Fingerprint ◽  
Dna Fingerprints ◽  
Evolutionary Studies ◽  
Pattern Characteristic ◽  
The Individual

Application of four DNA fingerprint probes to avocado (Persea americana Mill.) resulted in identification of various cultivars, characterization of the three avocado races, and a genetic analysis of family structure. Genomic DNA from 14 cultivars was probed with four DNA fingerprint probes. Three of the probes gave well-resolved bands. The individual-specific patterns obtained for each cultivar validate the use of this technique for definitive cultivar characterization, with the probability of obtaining a similar pattern for two different cultivars being 2 × 10-9. DNA mixes representing either Mexican, Guatemalan, or West-Indian avocado races were hybridized with the DNA fingerprint probes, and a band pattern characteristic for each race was obtained. Progeny of a cross between the cultivars Ettinger and Pinkerton were analyzed. Their DNA fingerprints revealed one pair of linked bands and another band allelic to one of them. The application of these observations to identification, evolutionary studies, and breeding is discussed.

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