VARIASI KOSENTRASI EKSTRAK ETANOL KULIT BUAH MANGGA HARUM MANIS (Mangifera indica L var. arum manis) TERHADAP FORMULA SEDIAAN GEL HAND SANITIZER SEBAGAI ANTIBAKTERI

Salah satu tanaman obat yang dapat dimanfaatkan sebagai sumber obat alami adalah buah mangga (Mangifera indica L var. arum manis) karena mengandung senyawa metabolit sekunder. Kulit mangga mengandung senyawa aktif penting seperti senyawa fenolik, karotenoid, flavonoid dan antosianin yang diketahui memiliki aktifitas sebagai antibakteri. Tujuan dari penelitian ini untuk mengetahui variasi kosentrasi berapakah yang berpontensi sebagai antibakteri . Pembuatan extrak etanol kulit buah mannga arum manis (Mangifera indica L var. arum manis) menggunakan metode maserasi dengan pelarut etanol 96%. Formulasi sediaan dibuat menggunakan Na.CMC sebagai gelling agent dengan konsentrasi 2%, 4%, 8%. Pengujian evaluasi sediaan hand sanitazer ekstrak kulit mangga meliputi uji organoleptis, uji homogenitas, uji pH, uji daya sebar, uji lekat, uji iritasi dan uji aktifitas antibakteri. Hasil penelitian menunjukkan bahwa sifat fisik dari sediaan gel memberikan pengaruh terhadap sifat fisik gel ekstrak kulit mangga (Mangifera indica L var. arum manis). Berdasarkan uji aktivitas antibakteri kosentrasi 2%, 4%, 8% yang paling kuat menujukan aktifitas antibakteri pada kosentrasi 8% dengan nilai rata-rata 18,28 mm.

The Combined Effect of Hot Water Treatment and Chitosan Coating on Mango (Mangifera indica L. cv. Kent) Fruits to Control Postharvest Deterioration and Increase Fruit Quality

The synergistic effect of dipping in 55 °C for 5 min of hot water (HW) and 1% chitosan coating during the storage of mango at 13 ± 0.5 °C and 85%–90% relative humidity for 28 days was investigated. The combined treatment significantly suppressed the fruit decay percentage compared with both the single treatment and the control. In addition, the specific activities of key plant defense-related enzymes, including peroxidase (POD) and catalase (CAT), markedly increased. The increase occurred in the pulp of the fruits treated with the combined treatment compared to those treated with HW or chitosan alone. While the control fruits showed the lowest values, the combination of pre-storage HW treatment and chitosan coating maintained higher values of flesh hue angle (h°), vitamin C content, membrane stability index (MSI) percentage, as well as lower weight loss compared with the untreated mango fruits. The combined treatment and chitosan treatment alone delayed fruit ripening by keeping fruit firmness, lessening the continuous increase of total soluble solids (TSS), and slowing the decrease in titratable acidity (TA). The results showed that the combined application of HW treatment and chitosan coating can be used as an effective strategy to suppress postharvest decay and improve the quality of mango fruits.

Design and characterization of a semi-open dynamic chamber for measuring biogenic volatile organic compound (BVOC) emissions from plants

With the accumulation of data about biogenic volatile organic compound (BVOC) emissions from plants based on branch-scale enclosure measurements worldwide, it is vital to assure that measurements are conducted using well-characterized dynamic chambers with good transfer efficiencies and less disturbance on natural growing microenvironments. In this study, a self-made cylindrical semi-open dynamic chamber with a Teflon-coated inner surface was characterized both in the lab with standard BVOC mixtures and in the field with typical broadleaf and coniferous trees. The lab simulation with a constant flow of standard mixtures and online monitoring of BVOCs by proton transfer reaction time-of-flight mass spectrometry (PTR-ToF-MS) revealed lower real-time mixing ratios and shorter equilibrium times than theoretically predicted due to wall loss in the chamber and that larger flow rates (shorter residence times) can reduce the adsorptive loss and improve the transfer efficiencies. However, even when flow rates were raised to secure residence times of less than 1 min, transfer efficiencies were still below 70 % for heavier BVOCs like α-pinene and β-caryophyllene. Relative humidity (RH) impacted the adsorptive loss of BVOCs less significantly when compared to flow rates, with compound-specific patterns related to the influence of RH on their adsorption behaviour. When the chamber was applied in the field to a branch of a Mangifera indica tree, the ambient–enclosure temperature differences decreased from 4.5±0.3 to 1.0±0.2 ∘C and the RH differences decreased from 9.8 ± 0.5 % to 1.2±0.1 % as flow rates increased from 3 L min−1 (residence time ∼4.5 min) to 15 L min−1 (residence time ∼0.9 min). At a medium flow rate of 9 L min−1 (residence time ∼1.5 min), field tests with the dynamic chamber for Mangifera indica and Pinus massoniana branches revealed enclosure temperature increase within +2 ∘C and CO2 depletion within −50 ppm when compared to their ambient counterparts. The results suggested that substantially higher air circulating rates would benefit by reducing equilibrium time, adsorptive loss, and the ambient–enclosure temperature and RH differences. However, even under higher air circulating rates and with inert Teflon-coated inner surfaces, the transfer efficiencies for monoterpene and sesquiterpene species are not so satisfactory, implying that emission factors for these species might be underestimated if they are obtained by dynamic chambers without certified transfer efficiencies and that further efforts are needed for field measurements to improve accuracies and narrow the uncertainties of the emission factors.

Production Process of a Nutritional Drink from Mango (Mangifera indica)

The aim of study was to determine optimal formulation and microwave parameter to pasteurize mango juice products to maintain the most nutrients and organoleptic level. Raw material and drinking product were assessed for properties including vitamin C content (mg/100ml), total polyphenol content (mgGAE/100ml) and antioxidant activity (% free radical scavenging ability). Besides, factors influencing the quality of mango juice including material to water ratio (w/w), material to syrup ratio (w/w), citric acid content (w/w), microware power (watt) and microware time (minutes) was examined. As a results, the mango:water dilution ratio as 1:2 (w/w); rate of mango:syrup as 1:2 (w/w); citric acid content as 0.3% (w/w); microwave power as 400 watt and microwave time of 3 minutes were suitable for achieving product with good nutritional and sensory quality. At these conditions, vitamin C content was 8.42 mg/100ml, total polyphenol content was 47.543 mgGAE/100ml, and DPPH free radical activity was 11.191%. The results also justified the role of mango as a new ingredient in beverage industry and suggested using microwave to pasteurize as an appropriate method for manufacturing drinking products from fruit.

SURVIVAL AND NATURAL REGENERATION OF FOREST ESSENCES CULTIVATED IN ALTERED AREAS THIRTY-FIVE YEARS AFTER PLANTING

The evaluation of the survival and natural regeneration of tree species in a 35-year-old plantation was carried out to identify the species established in the area, aiming at their recommendation in forest restoration plantations in the State of Acre. In the 1980s, 138 forest species were planted in two experimental units (EU), of 1.38 ha each, in the Zoobotanical Park (ZP) of the Federal University of Acre, Rio Branco campus. The main activities carried out in the area, prior to planting, were agriculture and cattle raising. Survival status was measured through a census of all individuals planted at the time, who were still alive. In addition, all regenerating individuals from planted species were surveyed in the effective planting area of the experimental units. At the time of evaluation, living individuals of 41 and 46 species were found in experimental units 1 (EU-1) and 2 (EU-2), respectively. The species Syagrus sancona, Talisia esculenta, Acacia polyphylla, Couepia bracteosa, Mangifera indica, Syzygium cumini and Copaifera multijuga showed survival rates above 90% in at least one of the experimental units. Only Syagrus sancona and Handroanthus serratifolius presented high survival rates in both experimental. Regenerating individuals of the species Aspidosperma vargasii, Couepia bracteosa, Euterpe precatoria, Handroanthus serratifolius, Oenocarpus mapora, Onychopetalum periquino and Stryphnodendron pulcherrimum were found in the two EU.