scholarly journals Application of DNA Fingerprints for Identification and Genetic Analyses of Mango (Mangifera indica) Genotypes

1995 ◽  
Vol 120 (2) ◽  
pp. 259-264 ◽  
Author(s):  
A. Adato ◽  
D. Sharon ◽  
U. Lavi ◽  
J. Hillel ◽  
S. Gazit
Keyword(s):  
Genetic Analysis ◽  
Genomic Dna ◽  
Genetic Relatedness ◽  
Mangifera Indica ◽  
Genetic Distances ◽  
Dna Fingerprint ◽  
Dna Fingerprints ◽  
Genetic Analyses ◽  
Young Leaves ◽  
Relatedness Analysis

DNA fingerprint information was used for identification of mango (Mangifera indica L.) cultivars for genetic relatedness analysis of20 mango cultivars and for genetic analysis of a family structure. Genomic DNA was extracted from young leaves, digested with Hind III or Dra I, and hybridized with 10 different DNA probes. Jeffreys' minisatellite probe 33.6 was the most useful, resulting in well-resolved bands representing highly polymorphic loci. Specific patterns were obtained for each cultivar. The probability of obtaining a similar pattern for two different cultivars was 9.4 × 10-6. Based on DNA fingerprint information, genetic distances between 20 mango cultivars were evaluated and an evolutionary tree was established. Analysis of DNA fingerprint band patterns of 12 progeny resulting from a cross between `Tommy Atkins' and `Keitt' mango revealed neither linked nor allelic bands. Application of the reported results for identification, genetic analyses, and mango breeding is discussed.

scholarly journals Application of DNA Fingerprints for Identification and Genetic Analysis of Avocado

1991 ◽  
Vol 116 (6) ◽  
pp. 1078-1081 ◽  
Author(s):  
U. Lavi ◽  
J. Hillel ◽  
A. Vainstein ◽  
E. Lahav ◽  
D. Sharon
Keyword(s):  
Genetic Analysis ◽  
Family Structure ◽  
Genomic Dna ◽  
Persea Americana ◽  
Dna Fingerprint ◽  
Dna Fingerprints ◽  
Evolutionary Studies ◽  
Pattern Characteristic ◽  
The Individual

Application of four DNA fingerprint probes to avocado (Persea americana Mill.) resulted in identification of various cultivars, characterization of the three avocado races, and a genetic analysis of family structure. Genomic DNA from 14 cultivars was probed with four DNA fingerprint probes. Three of the probes gave well-resolved bands. The individual-specific patterns obtained for each cultivar validate the use of this technique for definitive cultivar characterization, with the probability of obtaining a similar pattern for two different cultivars being 2 × 10-9. DNA mixes representing either Mexican, Guatemalan, or West-Indian avocado races were hybridized with the DNA fingerprint probes, and a band pattern characteristic for each race was obtained. Progeny of a cross between the cultivars Ettinger and Pinkerton were analyzed. Their DNA fingerprints revealed one pair of linked bands and another band allelic to one of them. The application of these observations to identification, evolutionary studies, and breeding is discussed.

scholarly journals Genetic Analysis of Inonotus Obliquus Strains by RAPD

2007 ◽  
Vol 26 (3) ◽  
pp. 201-205
Author(s):  
Yan-Qiu Chen ◽  
Xiao-Fan Guo ◽  
Chang-Tian Li ◽  
Yu Li
Keyword(s):  
Genetic Diversity ◽  
Genetic Variability ◽  
Genetic Analysis ◽  
Genetic Distances ◽  
Dna Fingerprints ◽  
Inonotus Obliquus ◽  
Medicinal Fungus ◽  
Different Strains ◽  
Rapd Fragment ◽  
Intraspecific Difference

Genetic Analysis ofInonotus ObliquusStrains by RAPDRAPD profiling of eightInonotus obliquusstrains isolated from sclerotia collected from different areas of China was conducted to determine the genetic variability within this important medicinal fungus and to better define relationships between the genotype and geographical origins of isolation. Twelve 10-mer primers generated a total of 167 stable and reproducible DNA fragments, of which 101 (60.5%) were polymorphic. DNA fingerprints revealed genetic diversity among the strains tested, but there was the little intraspecific difference between the fingerprints of individual strains. A phenogram constructed based on UPGMA analysis of genetic distances calculated from RAPD fragment data identified three distinct groupings: (1) BCX01 and BCX02, (2) JL01, JL02, JL03, JL04 and JL05, (3) HLJ01. Our data confirm that the genetic variability among different strains may be a useful ancillary tool for identifyingl. obliquussclerotia of different geographical origins.

scholarly journals Analysis of grape rootstocks by SSR markers

OENO One ◽  
2011 ◽  
Vol 45 (4) ◽  
pp. 199
Author(s):  
Gizella Jahnke ◽  
Gitta Kocsisné Molnár ◽  
János Májer ◽  
Barna Szöke ◽  
Erik Tarczal ◽  
...  
Keyword(s):  
Genetic Relatedness ◽  
Genetic Distances ◽  
Linkage Groups ◽  
High Similarity ◽  
Ssr Analysis ◽  
Young Leaves ◽  
High Level ◽  
Close Relatedness ◽  
Vitis Sylvestris

<p style="text-align: justify;"><strong>Aims</strong>: The aim of this work was to determine the SSR profile of 96 <em>Vitis</em> accessions (mainly rootstocks) at 19 loci to find genetic relatedness between them.</p><p style="text-align: justify;"><strong>Methods and results</strong>: The molecular markers used in the last 30 years are not or less affected by the environment, making them a valuable tool for the determination of genetic distances. Based on our former experiences, an SSR analysis was carried out on 96 grape accretions, mainly rootstocks. DNA was extracted from young leaves and analyses were carried out at 19 microsatellite loci. The loci were selected to cover all of the linkage groups to get a more accurate genotyping of each grape rootstock. Results of the SSR analysis and the resulting dendrogram showing genetic relatedness are presented.</p><p style="text-align: justify;"><strong>Conclusions</strong>: Based on the results, it can be established, that most of the selected loci showed appropriate polymorphism for the assessment of genetic relatedness. The rootstocks derived from Teleki’s seedlings showed high similarity. A low genetic distance was also detected between the accessions originated from the cross between Börner and Georgikon 28. Finally, the <em>Vitis sylvestris</em> accessions showed close relatedness with the <em>Vitis vinifera</em> L. varieties.</p><p style="text-align: justify;"><strong>Significance and impact of the study</strong>: Our results indicate a high level of polymorphism between grape rootstock accessions. Strong differences were detected between the so-called Teleki 5C (T5C) and Teleki-Kober 5BB (T5KBB) “clones”, suggesting that they are not real clones but different genotypes with highly similar morphological features.</p>

DNA fingerprinting analysis ofPetromyces alliaceus(AspergillussectionFlavi)

2005 ◽  
Vol 51 (12) ◽  
pp. 1039-1044 ◽  
Author(s):  
Cesaria E McAlpin ◽  
Donald T Wicklow
Keyword(s):  
Dna Fingerprinting ◽  
Aspergillus Flavus ◽  
Genomic Dna ◽  
Genotypic Diversity ◽  
Dna Probe ◽  
Dna Fingerprint ◽  
Dna Fingerprints ◽  
Close Relationship ◽  
Aspergillus Alliaceus ◽  
Aspergillus Section

The objective of this study was to evaluate the ability of the Aspergillus flavus pAF28 DNA probe to produce DNA fingerprints for distinguishing among genotypes of Petromyces alliaceus (Aspergillus section Flavi), a fungus considered responsible for the ochratoxin A contamination that is occasionally observed in California fig orchards. P. alliaceus (14 isolates), Petromyces albertensis (one isolate), and seven species of Aspergillus section Circumdati (14 isolates) were analyzed by DNA fingerprinting using a repetitive sequence DNA probe pAF28 derived from A. flavus. The presence of hybridization bands with the DNA probe and with the P. alliaceus or P. albertensis genomic DNA indicates a close relationship between A. flavus and P. alliaceus. Twelve distinct DNA fingerprint groups or genotypes were identified among the 15 isolates of Petromyces. Conspecificity of P. alliaceus and P. albertensis is suggested based on DNA fingerprints. Species belonging to Aspergillus section Circumdati hybridized only slightly at the 7.0-kb region with the repetitive DNA probe, unlike the highly polymorphic hybridization patterns obtained from P. alliaceus and A. flavus, suggesting very little homology of the probe to Aspergillus section Circum dati genomic DNA. The pAF28 DNA probe offers a tool for typing and monitoring specific P. alliaceus clonal populations and for estimating the genotypic diversity of P. alliaceus in orchards, vineyards, or crop fields.Key words: Aspergillus alliaceus, Circumdati, DNA probe, genotypic diversity, hybridization patterns, ochratoxin, Southern blot.

scholarly journals Genetic Diversity and Population Structures in Chinese Miniature Pigs Revealed by SINE Retrotransposon Insertion Polymorphisms, a New Type of Genetic Markers

Animals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 1136
Author(s):  
Cai Chen ◽  
Xiaoyan Wang ◽  
Wencheng Zong ◽  
Enrico D’Alessandro ◽  
Domenico Giosa ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Genetic Markers ◽  
Structure Analysis ◽  
Genetic Distances ◽  
Population Genetic Analysis ◽  
Miniature Pigs ◽  
Population Structure Analysis ◽  
Information Contents ◽  
Population Structures ◽  
New Type

RIPs have been developed as effective genetic markers and popularly applied for genetic analysis in plants, but few reports are available for domestic animals. Here, we established 30 new molecular markers based on the SINE RIPs, and applied them for population genetic analysis in seven Chinese miniature pigs. The data revealed that the closed herd (BM-clo), inbreeding herd (BM-inb) of Bama miniature pigs were distinctly different from the BM-cov herds in the conservation farm, and other miniature pigs (Wuzhishan, Congjiang Xiang, Tibetan, and Mingguang small ear). These later five miniature pig breeds can further be classified into two clades based on a phylogenetic tree: one included BM-cov and Wuzhishan, the other included Congjiang Xiang, Tibetan, and Mingguang small ear, which was well-supported by structure analysis. The polymorphic information contents estimated by using SINE RIPs are lower than the predictions based on microsatellites. Overall, the genetic distances and breed-relationships between these populations revealed by 30 SINE RIPs generally agree with their evolutions and geographic distributions. We demonstrated the potential of SINE RIPs as new genetic markers for genetic monitoring and population structure analysis in pigs, which can even be extended to other livestock animals.

scholarly journals Moscato Cerletti, a rediscovered aromatic cultivar with oenological potential in warm and dry areas

OENO One ◽  
2021 ◽  
Vol 55 (3) ◽  
pp. 123-140
Author(s):  
Antonio Sparacio ◽  
Francesco Mercati ◽  
Filippo Sciara ◽  
Antonino Pisciotta ◽  
Felice Capraro ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Historical Documents ◽  
Genetic Analyses ◽  
Changing Climate ◽  
Dry Areas ◽  
Storage Ability

Baron Antonio Mendola was devoted to the study of grapevine, applying ampelography and dabbling in crosses between cultivars in order to select new ones, of which Moscato Cerletti, obtained in 1869, was the most interesting. Grillo, one of the most important white cultivars in Sicily, was ascertained to be an offspring of Catarratto Comune and Zibibbo, the same parents which Mendola claimed he used to obtain Moscato Cerletti. Thus the hypothesis of synonymy between Moscato Cerletti and Grillo or the same parentage for both sets of parents needs to be verified.In the present study, historical documents were consulted and genetic analyses and ampelographic, agronomic and qualitative characterisation carried out to determine the distinctiveness of each cultivars. These were also compared with Catarratto Comune and Zibibbo in order to establish the Moscato Cerletti pedigree. Due to their different SSR profiles, Grillo and Moscato Cerletti were confirmed as two distinct cultivars; they also differed in ripening times and sugar storage ability, as well as in the aromatic grape produced by Moscato Cerletti only. The trio genotype genetic analysis confirmed that Zibibbo is a parent of Moscato Cerletti (justifying the aromatic grape), whilst the SSR profiles did not show Catarratto Comune to be a second parent.Moscato Cerletti was found to have oenological potential in the production of sparkling muscat wines due to its ability to adapt to a changing climate in warm and dry environments and in different winegrowing regions.

scholarly journals Genetic analysis of silver-fir populations in the Beskids

2011 ◽  
Vol 72 (2) ◽  
pp. 115-119 ◽  
Author(s):  
Leon Mejnartowicz
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Genetic Analysis ◽  
Abies Alba ◽  
Genetic Distances ◽  
Silver Fir ◽  
Geographical Distance ◽  
Polymorphic Loci ◽  
Expected Heterozygosity ◽  
Rare Alleles

Twenty-eight isozymic loci were studied in the Beskid Mts., in four populations of common silver-fir (<em>Abies alba</em>): one in Beskid Makowski (BM) and three populations in Beskid Sądecki (BS). Their genetic variation and diversity were analyzed, and Nei's genetic distances between the populations were calculated. The results show that the geographical distance between the BM population and the three BS populations is reflected in genetic distances. The BM population is clearly distinct from the others. It has the lowest genetic diversity (<em>I</em> = <em>0.42</em>), percentage of polymorphic loci <em>(%PoL </em>= <em>64.29</em>) and number of rare alleles (<em>NoRa </em>= <em>5</em>). Besides, the BM population has the highest observed heterozygosity (<em>Ho </em>= <em>0.291</em>), which exceeds the expected heterozygosity (<em>He </em>= <em>0.254</em>), estimated on the basis of the Hardy-Weinberg Principle. On the contrary, BS populations are in the state of equilibrium, which is manifested, in similar values of <em>He </em>= <em>0.262 </em>and <em>Ho </em>= <em>0.264</em>.

scholarly journals Isolation of Genomic DNA from Claw Clippings for Genetic Analysis in Chickens

BioTechniques ◽  
1997 ◽  
Vol 22 (5) ◽  
pp. 874-876 ◽  
Author(s):  
P.B. Drummond ◽  
Y. Song ◽  
D. Vaughn ◽  
A. Gbadamosi ◽  
K. Butler ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Genomic Dna

The genetic relatedness analysis of male sterile and their maintainer lines of onion (Allium cepa L.) by using RAPD primers

2013 ◽  
Vol 16 (1) ◽  
pp. 29-33 ◽  
Author(s):  
Dhanya Shivnanajappa ◽  
Lakshmana Reddy Dhoranalapalli Chinnappareddy ◽  
Verre Gowda ◽  
Sreedhara Sindughatta Antharamiah ◽  
Aswath Chennareddy
Keyword(s):  
Allium Cepa ◽  
Genetic Relatedness ◽  
Male Sterile ◽  
Allium Cepa L ◽  
Relatedness Analysis

A new species of Procontarinia (Diptera: Cecidomyiidae) damaging fruit of mango, Mangifera indica (Anacardiaceae), in China

Zootaxa ◽  
2018 ◽  
Vol 4413 (2) ◽  
pp. 368
Author(s):  
KE-LONG JIAO ◽  
HAO WANG ◽  
DE-WEI WEI ◽  
JIAN-YOU MO ◽  
YUAN-HONG WANG ◽  
...  
Keyword(s):  
New Species ◽  
Southern China ◽  
Mitochondrial Gene ◽  
Mangifera Indica ◽  
Gall Midge ◽  
Gene Segment ◽  
The Philippines ◽  
Unknown Species ◽  
Young Leaves ◽  
Basic Biology

Larvae of a previously unknown species of gall midge were found feeding on young fruit of mango, Mangifera indica (Anacardiaceae), in Guangxi Autonomous Region in southern China, causing severe damage to the crop. The new species is named Procontarinia fructiculi Jiao, Wang, Bu & Kolesik, its morphology is described, the basic biology is given, and the Cytochrome Oxidase subunit I (COI) mitochondrial gene segment is sequenced and compared to other congeners. Procontarinia contains now 16 described species, each feeding on mango. All but three species cause variously shaped galls on leaves, while P. mangiferae (Felt) malforms inflorescence and young leaves, and two species feed on fruit – P. frugivora Gagné causing deep lesions and P. fructiculi sp. nov. tunnel-like holes. Of the two fruit-feeding species, P. frugivora is confined to the Philippines while the new species has thus far been recorded only from southern China. 

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