Oligonucleotide-priming methods for the chromosome-specific labelling of alpha satellite DNA in situ

Chromosoma ◽  
1989 ◽  
Vol 98 (4) ◽  
pp. 259-265 ◽  
Author(s):  
J�rn E. Koch ◽  
Steen K�lvraa ◽  
Kirsten B. Petersen ◽  
Niels Gregersen ◽  
Lars Bolund
Keyword(s):  
Satellite Dna ◽  
Alpha Satellite ◽  
Alpha Satellite Dna ◽  
Specific Labelling

scholarly journals Dynamic elastic behavior of alpha-satellite DNA domains visualized in situ in living human cells.

1996 ◽  
Vol 135 (3) ◽  
pp. 545-557 ◽  
Author(s):  
R D Shelby ◽  
K M Hahn ◽  
K F Sullivan
Keyword(s):  
Satellite Dna ◽  
Fluorescent Protein ◽  
Time Lapse ◽  
Fluorescent Labeling ◽  
Human Cells ◽  
Elastic Behavior ◽  
Alpha Satellite ◽  
Alpha Satellite Dna ◽  
Green Fluorescent

We have constructed a fluorescent alpha-satellite DNA-binding protein to explore the motile and mechanical properties of human centromeres. A fusion protein consisting of human CENP-B coupled to the green fluorescent protein (GFP) of A. victoria specifically targets to centromeres when expressed in human cells. Morphometric analysis revealed that the alpha-satellite DNA domain bound by CENPB-GFP becomes elongated in mitosis in a microtubule-dependent fashion. Time lapse confocal microscopy in live mitotic cells revealed apparent elastic deformations of the central domain of the centromere that occurred during metaphase chromosome oscillations. These observations demonstrate that the interior region of the centromere behaves as an elastic element that could play a role in the mechanoregulatory mechanisms recently identified at centromeres. Fluorescent labeling of centromeres revealed that they disperse throughout the nucleus in a nearly isometric expansion during chromosome decondensation in telophase and early G1. During interphase, centromeres were primarily stationary, although motility of individual or small groups of centromeres was occasionally observed at very slow rates of 7-10 microns/h.

Detection of an i(17q) chromosome by fluorescent in situ hybridization with a chromosome 17 alpha satellite DNA probe

1992 ◽  
Vol 62 (2) ◽  
pp. 140-143 ◽  
Author(s):  
Hitoshi Nakagawa ◽  
Johji Inazawa ◽  
Shinichi Misawa ◽  
Shinji Tanaka ◽  
Teruyuki Takashima ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Satellite Dna ◽  
Fluorescent In Situ Hybridization ◽  
Dna Probe ◽  
Chromosome 17 ◽  
Alpha Satellite ◽  
Alpha Satellite Dna

Flow-cytometric quantification in human gliomas of alpha satellite DNA sequences specific for chromosome 7 using fluorescence in situ hybridization

Cytometry ◽  
1994 ◽  
Vol 17 (1) ◽  
pp. 26-32 ◽  
Author(s):  
Taeshik Kwak ◽  
Takafumi Nishizaki ◽  
Haruhide Ito ◽  
Yuka Kimura ◽  
Tomoyuki Murakami ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Dna Sequences ◽  
Satellite Dna ◽  
Chromosome 7 ◽  
Alpha Satellite ◽  
Human Gliomas ◽  
Alpha Satellite Dna ◽  
Flow Cytometric

On the mode of evolution of alpha satellite DNA in human populations

1991 ◽  
Vol 33 (1) ◽  
pp. 42-48 ◽  
Author(s):  
B. Marçais ◽  
J. P. Charlieu ◽  
B. Allain ◽  
E. Brun ◽  
M. Bellis ◽  
...  
Keyword(s):  
Satellite Dna ◽  
Human Populations ◽  
Alpha Satellite ◽  
Alpha Satellite Dna

Efficient identification of marker chromosomes in 27 patients by stepwise hybridization with alpha-satellite DNA probes

1993 ◽  
Vol 91 (2) ◽  
Author(s):  
Rina Plattner ◽  
NylaA. Heerema ◽  
YuriB. Yurov ◽  
CatherineG. Palmer
Keyword(s):  
Satellite Dna ◽  
Dna Probes ◽  
Alpha Satellite ◽  
Marker Chromosomes ◽  
Alpha Satellite Dna

Structure of DNA near long tandem arrays of alpha satellite DNA at the centromere of human chromosome 7

Genomics ◽  
1992 ◽  
Vol 14 (4) ◽  
pp. 912-923 ◽  
Author(s):  
Rachel Wevrick ◽  
Vicki P. Willard ◽  
Huntington F. Willard
Keyword(s):  
Human Chromosome ◽  
Satellite Dna ◽  
Chromosome 7 ◽  
Alpha Satellite ◽  
Alpha Satellite Dna ◽  
Human Chromosome 7 ◽  
Tandem Arrays
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