DNA-mediated gene transfer in Chinese hamster ovary cells: Clonal variation in transfer efficiency

1982 ◽  
Vol 187 (3) ◽  
pp. 384-390 ◽  
Author(s):  
Rodney S. Nairn ◽  
Gerald M. Adair ◽  
Ronald M. Humphrey
Keyword(s):  
Gene Transfer ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Transfer Efficiency ◽  
Clonal Variation ◽  
Ovary Cells

Complementation of a methotrexate uptake defect in Chinese hamster ovary cells by DNA-mediated gene transfer

1989 ◽  
Vol 9 (4) ◽  
pp. 1754-1758
Author(s):  
T M Underhill ◽  
W F Flintoff
Keyword(s):  
Gene Transfer ◽  
Dna Sequences ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Ovary Cell ◽  
Wild Type ◽  
Ovary Cells ◽  
Ovary Cell Line ◽  
Human Specific

A methotrexate-resistant Chinese hamster ovary cell line deficient in methotrexate uptake has been complemented to methotrexate sensitivity by transfection with DNA isolated from either wild-type Chinese hamster ovary or human G2 cells. Primary and secondary transfectants regained the ability to take up methotrexate in a manner similar to that of wild-type cells, and in the case of those transfected with human DNA, to contain human-specific DNA sequences. The complementation by DNA-mediated gene transfer of this methotrexate-resistant phenotype provides a basis for the cloning of a gene involved in methotrexate uptake.

Targeting vector configuration and method of gene transfer influence targeted correction of theAPRT gene in Chinese hamster ovary cells

1993 ◽  
Vol 19 (4) ◽  
pp. 363-375 ◽  
Author(s):  
Rodney S. Nairn ◽  
Gerald M. Adair ◽  
Thomas Porter ◽  
Sandra L. Pennington ◽  
Debra G. Smith ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Ovary Cells ◽  
Targeting Vector

LINE‐1 vectors mediate recombinant antibody gene transfer by retrotransposition in Chinese hamster ovary cells

2021 ◽  
pp. 2000620
Author(s):  
Feiyang Zheng ◽  
Yoshinori Kawabe ◽  
Mai Murakami ◽  
Mamika Takahashi ◽  
Kyoka Nishihata ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Recombinant Antibody ◽  
Chinese Hamster ◽  
Ovary Cells ◽  
Antibody Gene

scholarly journals Complementation of a methotrexate uptake defect in Chinese hamster ovary cells by DNA-mediated gene transfer.

1989 ◽  
Vol 9 (4) ◽  
pp. 1754-1758 ◽  
Author(s):  
T M Underhill ◽  
W F Flintoff
Keyword(s):  
Gene Transfer ◽  
Dna Sequences ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Ovary Cell ◽  
Wild Type ◽  
Ovary Cells ◽  
Ovary Cell Line ◽  
Human Specific

A methotrexate-resistant Chinese hamster ovary cell line deficient in methotrexate uptake has been complemented to methotrexate sensitivity by transfection with DNA isolated from either wild-type Chinese hamster ovary or human G2 cells. Primary and secondary transfectants regained the ability to take up methotrexate in a manner similar to that of wild-type cells, and in the case of those transfected with human DNA, to contain human-specific DNA sequences. The complementation by DNA-mediated gene transfer of this methotrexate-resistant phenotype provides a basis for the cloning of a gene involved in methotrexate uptake.

Malignancy-related characteristics of wild type and drug-resistant chinese hamster ovary cells

Pathology ◽  
1993 ◽  
Vol 25 (3) ◽  
pp. 268-276 ◽  
Author(s):  
Wanda B. Mackinnon ◽  
Marlen Dyne ◽  
Rebecca Hancock ◽  
Carolyn E. Mountford ◽  
Adrienne J. Grant ◽  
...  
Keyword(s):  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Drug Resistant ◽  
Wild Type ◽  
Ovary Cells
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