LINE‐1 vectors mediate recombinant antibody gene transfer by retrotransposition in Chinese hamster ovary cells

2021 ◽  
pp. 2000620
Author(s):  
Feiyang Zheng ◽  
Yoshinori Kawabe ◽  
Mai Murakami ◽  
Mamika Takahashi ◽  
Kyoka Nishihata ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Recombinant Antibody ◽  
Chinese Hamster ◽  
Ovary Cells ◽  
Antibody Gene

Complementation of a methotrexate uptake defect in Chinese hamster ovary cells by DNA-mediated gene transfer

1989 ◽  
Vol 9 (4) ◽  
pp. 1754-1758
Author(s):  
T M Underhill ◽  
W F Flintoff
Keyword(s):  
Gene Transfer ◽  
Dna Sequences ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Ovary Cell ◽  
Wild Type ◽  
Ovary Cells ◽  
Ovary Cell Line ◽  
Human Specific

A methotrexate-resistant Chinese hamster ovary cell line deficient in methotrexate uptake has been complemented to methotrexate sensitivity by transfection with DNA isolated from either wild-type Chinese hamster ovary or human G2 cells. Primary and secondary transfectants regained the ability to take up methotrexate in a manner similar to that of wild-type cells, and in the case of those transfected with human DNA, to contain human-specific DNA sequences. The complementation by DNA-mediated gene transfer of this methotrexate-resistant phenotype provides a basis for the cloning of a gene involved in methotrexate uptake.

Targeted knock-in of an scFv-Fc antibody gene into the hprt locus of Chinese hamster ovary cells using CRISPR/Cas9 and CRIS-PITCh systems

2018 ◽  
Vol 125 (5) ◽  
pp. 599-605 ◽  
Author(s):  
Yoshinori Kawabe ◽  
Shinya Komatsu ◽  
Shodai Komatsu ◽  
Mai Murakami ◽  
Akira Ito ◽  
...  
Keyword(s):  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Ovary Cells ◽  
Hprt Locus ◽  
Antibody Gene

scholarly journals Biological insights into the expression of translation initiation factors from recombinant CHOK1SV cell lines and their relationship to enhanced productivity

2015 ◽  
Vol 472 (3) ◽  
pp. 261-273 ◽  
Author(s):  
Emma J. Mead ◽  
Rosalyn J. Masterton ◽  
Marc Feary ◽  
Olga Obrezanova ◽  
Lin Zhang ◽  
...  
Keyword(s):  
Translation Initiation ◽  
Chinese Hamster Ovary ◽  
Closed Loop ◽  
Chinese Hamster Ovary Cells ◽  
Recombinant Antibody ◽  
Chinese Hamster ◽  
Ovary Cells ◽  
Initiation Factors ◽  
Translation Initiation Factors ◽  
Antibody Productivity

We show for translation initiation factors involved in formation of the closed loop mRNA, their expression is associated with recombinant antibody productivity in Chinese hamster ovary cells and maintaining these is important in determining the cells capacity for antibody productivity.

Targeting vector configuration and method of gene transfer influence targeted correction of theAPRT gene in Chinese hamster ovary cells

1993 ◽  
Vol 19 (4) ◽  
pp. 363-375 ◽  
Author(s):  
Rodney S. Nairn ◽  
Gerald M. Adair ◽  
Thomas Porter ◽  
Sandra L. Pennington ◽  
Debra G. Smith ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Ovary Cells ◽  
Targeting Vector

scholarly journals Complementation of a methotrexate uptake defect in Chinese hamster ovary cells by DNA-mediated gene transfer.

1989 ◽  
Vol 9 (4) ◽  
pp. 1754-1758 ◽  
Author(s):  
T M Underhill ◽  
W F Flintoff
Keyword(s):  
Gene Transfer ◽  
Dna Sequences ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Ovary Cell ◽  
Wild Type ◽  
Ovary Cells ◽  
Ovary Cell Line ◽  
Human Specific

A methotrexate-resistant Chinese hamster ovary cell line deficient in methotrexate uptake has been complemented to methotrexate sensitivity by transfection with DNA isolated from either wild-type Chinese hamster ovary or human G2 cells. Primary and secondary transfectants regained the ability to take up methotrexate in a manner similar to that of wild-type cells, and in the case of those transfected with human DNA, to contain human-specific DNA sequences. The complementation by DNA-mediated gene transfer of this methotrexate-resistant phenotype provides a basis for the cloning of a gene involved in methotrexate uptake.

Malignancy-related characteristics of wild type and drug-resistant chinese hamster ovary cells

Pathology ◽  
1993 ◽  
Vol 25 (3) ◽  
pp. 268-276 ◽  
Author(s):  
Wanda B. Mackinnon ◽  
Marlen Dyne ◽  
Rebecca Hancock ◽  
Carolyn E. Mountford ◽  
Adrienne J. Grant ◽  
...  
Keyword(s):  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Drug Resistant ◽  
Wild Type ◽  
Ovary Cells
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