Both TATA box and upstream regions are required for the nopaline synthase promoter activity in transformed tobacco cells

1986 ◽  
Vol 203 (2) ◽  
pp. 245-250 ◽  
Author(s):  
Gynheung An ◽  
Paul R. Ebert ◽  
Bu-Young Yi ◽  
Chul-Hi Choi
Keyword(s):  
Promoter Activity ◽  
Tata Box ◽  
Tobacco Cells ◽  
Transformed Tobacco

In transformed tobacco cells the apoplasmic invertase inhibitor operates as a regulatory switch of cell wall invertase

1998 ◽  
Vol 13 (2) ◽  
pp. 275-280 ◽  
Author(s):  
Silke Krausgrill ◽  
Steffen Greiner ◽  
Ulrike Köster ◽  
Rolf Vogel ◽  
Thomas Rausch
Keyword(s):  
Cell Wall ◽  
Cell Wall Invertase ◽  
Tobacco Cells ◽  
Invertase Inhibitor ◽  
Transformed Tobacco

Sugar transport into plasma membrane vesicles of transformed tobacco cells: uptake of sucrose but not of hexoses is stimulated by ΔpH

1994 ◽  
Vol 45 (2) ◽  
pp. 245-252
Author(s):  
Thomas Rausch ◽  
Martina Bö ◽  
Ingo Dahse ◽  
Marion Weil ◽  
Reinhold Verstappen
Keyword(s):  
Plasma Membrane ◽  
Sugar Transport ◽  
Membrane Vesicles ◽  
Plasma Membrane Vesicles ◽  
Tobacco Cells ◽  
Transformed Tobacco

scholarly journals Cell-specific expression of the human gastrin gene: evidence for a control element located downstream of the TATA box.

1987 ◽  
Vol 7 (12) ◽  
pp. 4329-4336 ◽  
Author(s):  
L E Theill ◽  
O Wiborg ◽  
J Vuust
Keyword(s):  
Cell Line ◽  
Promoter Activity ◽  
Gene Promoter ◽  
Tata Box ◽  
Vector System ◽  
Control Element ◽  
Gene Fragment ◽  
Early Promoter ◽  
Sv40 Enhancer ◽  
Human Gastrin

Fragments of 5'-flanking and noncoding exon I sequences of the human gastrin gene were analyzed in transient expression assays after transfection of a variety of cell lines with the pSVCAT vector system. In the presence of the simian virus 40 (SV40) enhancer, the gastrin gene fragment from nucleotides -250 to +57, relative to the cap site, was as efficient a promoter as the SV40 early promoter itself. In the absence of the SV40 enhancer, gastrin gene 5'-flanking sequences had no promoter activity except in the murine neuroblastoma cell line N18TG2. In this cell line, the fragment from -1300 to +57 stimulated transcription as actively as the SV40 early promoter with its enhancer. This cell-specific gastrin gene promoter activity was in accordance with the finding that gastrin is synthesized in certain neuronal cells. Promoter activity declined with decreasing distance from the 5' end to the cap site and disappeared after removal of the gastrin gene TATA box. In vector constructions containing short vector-linker sequences homologous to a functionally important region of the SV40 enhancer, the gastrin gene fragment from -17 to +57 showed considerable promoter activity, exclusively in N18TG2. It is concluded that the truncated gastrin gene promoter plus the first exon contains a cell-specific element that may act in collaboration with upstream elements to facilitate the accumulation of transcripts.

scholarly journals Regulation of human prohormone convertase 2 promoter activity by the transcription factor EGR-1

1997 ◽  
Vol 328 (1) ◽  
pp. 69-74 ◽  
Author(s):  
Erik JANSEN ◽  
A. Y. Torik AYOUBI ◽  
M. P. Sandra MEULEMANS ◽  
Wim J. M. VAN DE VEN
Keyword(s):  
Transcription Factor ◽  
Promoter Region ◽  
Promoter Activity ◽  
Secretory Pathway ◽  
Tata Box ◽  
Proximal Promoter ◽  
Prohormone Convertase ◽  
Specific Expression ◽  
Proximal Promoter Region ◽  
Prohormone Convertase 2

Prohormone convertases are involved in the tissue-specific endoproteolytic processing of prohormones and neuropeptide precursors within the secretory pathway. In the present study, we have isolated genomic clones comprising the 5ʹ-terminal region of the human prohormone convertase 2 (PC2) gene and established characteristics of the PC2 promoter region. The proximal promoter region is very G+C-rich and does not contain a canonical TATA box or a CAAT box. Transient expression assays with a set of human PC2 gene fragments containing progressive 5ʹ deletions demonstrate that the proximal promoter region is capable of directing high levels of neuroendocrine-specific expression of reporter gene constructs. In addition, we show that the transcription factor EGR-1 interacts with two distinct elements within the proximal human PC2 promoter region. Transfection experiments also demonstrate that EGR-1 is able to enhance PC2 promoter activity.

scholarly journals Interaction of a lens cell transcription factor with the proximal domain of the mouse gamma F-crystallin promoter.

1991 ◽  
Vol 11 (3) ◽  
pp. 1531-1537 ◽  
Author(s):  
Q R Liu ◽  
M Tini ◽  
L C Tsui ◽  
M L Breitman
Keyword(s):  
Transcription Factor ◽  
Promoter Activity ◽  
Transcriptional Activator ◽  
Tata Box ◽  
Specific Expression ◽  
Nuclear Factors ◽  
Gamma Crystallin ◽  
Lens Cells ◽  
Lens Cell

The elements regulating lens-specific expression of the mouse gamma F-crystallin gene were examined. Here we show that mouse gamma F-crystallin sequences -67 to +45 contain a low basal level of lens-specific promoter activity and that sequences -67 to -25, which are highly conserved among different gamma-crystallin genes, are able to function as a strong transcriptional activator when duplicated and placed upstream of the TATA box. We also show that nuclear factors from lens and nonlens cells are able to form different complexes with sequences centered at -46 to -36 and demonstrate that binding of the factor from lens cells correlates with lens-specific promoter activity of the mouse gamma F-crystallin gene.

scholarly journals Characterization of cis-Acting and NS1 Protein-Responsive Elements in the p6 Promoter of Parvovirus B19

1998 ◽  
Vol 72 (1) ◽  
pp. 609-616 ◽  
Author(s):  
Ralph Gareus ◽  
Andreas Gigler ◽  
Andrea Hemauer ◽  
Marianne Leruez-Ville ◽  
Frédéric Morinet ◽  
...  
Keyword(s):  
Cell Line ◽  
Promoter Activity ◽  
Transcriptional Activity ◽  
Parvovirus B19 ◽  
K562 Cells ◽  
Tata Box ◽  
Erythroid Cell ◽  
Ns1 Protein ◽  
Erythroid Progenitor ◽  
Virus Isolates

ABSTRACT Parvovirus B19 infections are associated with diverse clinical manifestations, ranging from no symptoms to severe symptoms. The virus shows an extreme tropism for replication in erythroid progenitor cells, possibly due to the activity of the only functional promoter (p6) of the B19 virus genome in combination with both cell- and cell cycle-specific factors and the trans-activator protein NS1. As presented here, p6 promoter sequences derived from several B19 virus isolates proved to be highly conserved. Furthermore, mutations did not affect any of the potential binding sites for transcription factors. One variation of the base at position 223 was identified only in B19 virus isolates derived from patients with persistent infection or chronic arthritis. To determine promoter activity and to characterize regulatory elements, sequences spanning the total p6 promoter and subfragments of them were introduced into a eukaryotic expression vector upstream of the luciferase gene (from Photinus pyralis). After transfection into HeLa, CEM, BJAB, and K562 cells, the p6 promoter was found to be highly active. When introduced into the erythroid cell line K562, p6-controlled transcription exceeded that of the simian virus 40 promoter-enhancer used as a control by more than 25-fold. Sequence elements relevant for promoter activity mapped to the regions from nucleotides (nt) 100 to 190 and 233 to 298. Also, the segment from nt 343 to 400 downstream of the TATA box was important for transcriptional activity in HeLa and K562 cells. By transfecting the promoter-luciferase constructs into a HeLa cell line stably carrying the viral NS1 gene under the control of an inducible promoter, transcriptional activity mediated by the p6 promoter rose significantly after induction of NS1 expression. The region from nt 100 to 160 proved to be essential for NS1-mediated transcriptional activation. Furthermore, NS1-mediated transactivation was dependent on the presence of two GC-rich elements arranged in tandem upstream of the TATA box. These data indicate that NS1-mediated p6 transactivation is dependent on a multicomponent complex combining NS1 with ATF, NF-κB/c-Rel, and GC-box binding cellular factors.

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