Alloreactive T-cell clones. Ly phenotypes predict both function and specificity for major histocompatibility complex products

1983 ◽  
Vol 17 (2) ◽  
pp. 147-165 ◽  
Author(s):  
Anjana Rao ◽  
W. Jeffrey Allard ◽  
Patrick G. Hogan ◽  
Rene S. Rosenson ◽  
Harvey Cantor
Keyword(s):  
Major Histocompatibility Complex ◽  
T Cell ◽  
Major Histocompatibility ◽  
T Cell Clones ◽  
Complex Products ◽  
Histocompatibility Complex ◽  
Cell Clones ◽  
Alloreactive T Cell

scholarly journals Specificity of T cell clones for antigen and autologous major histocompatibility complex products determines specificity for foreign major histocompatibility complex products.

1983 ◽  
Vol 158 (2) ◽  
pp. 428-437 ◽  
Author(s):  
S R Abromson-Leeman ◽  
H Cantor
Keyword(s):  
Major Histocompatibility Complex ◽  
T Cell ◽  
Molecular Mimicry ◽  
Class Ii ◽  
Major Histocompatibility ◽  
T Cell Clones ◽  
Complex Products ◽  
Mhc Molecules ◽  
Histocompatibility Complex ◽  
Cell Clones

We have analyzed a panel of T cell clones that corecognize defined epitopes of the insulin molecule in association with Ia for their patterns of recognition of alloantigens. A striking correlation is observed between recognition of the I-Ab gene product and cow insulin alpha loop and recognition of I-Eu of the PL/J haplotype. These results are consistent with the notion that reactions to foreign major histocompatibility complex (MHC) products reflect molecular mimicry by foreign class II antigens of 'physiologic' complexes formed by autologous class II MHC molecules and antigen.

scholarly journals T cell clones with dual specificity for M1s and various major histocompatibility complex determinants.

1981 ◽  
Vol 154 (6) ◽  
pp. 1970-1974 ◽  
Author(s):  
S R Webb ◽  
K Molnar-Kimber ◽  
J Bruce ◽  
J Sprent ◽  
D B Wilson
Keyword(s):  
Major Histocompatibility Complex ◽  
T Cell ◽  
Major Histocompatibility ◽  
T Cell Clones ◽  
Histocompatibility Complex ◽  
Cell Clones ◽  
Dual Specificity

A high proportion of T cell clones derived from bulk cultures selected to M1s a,d determinants were found to have joint specificity for allo-H-2 determinants, and vice versa. Significantly, the patterns of H-2 alloreactivity shown by clones selected to M1sa,b determinants appeared to be random. The possible implications of these findings are discussed.

scholarly journals The molecular basis of alloreactivity in antigen-specific, major histocompatibility complex-restricted T cell clones

Cell ◽  
1987 ◽  
Vol 51 (1) ◽  
pp. 59-69 ◽  
Author(s):  
Louis A. Matis ◽  
Simona B. Sorger ◽  
David L. McElligott ◽  
Pamela J. Fink ◽  
Stephen M. Hedrick
Keyword(s):  
Major Histocompatibility Complex ◽  
T Cell ◽  
Molecular Basis ◽  
Major Histocompatibility ◽  
T Cell Clones ◽  
Histocompatibility Complex ◽  
Cell Clones

scholarly journals Major Histocompatibility Complex–independent Recognition of a Distinctive Pollen Antigen, Most Likely a Carbohydrate, by Human CD8+ α/β T Cells

1997 ◽  
Vol 186 (6) ◽  
pp. 899-908 ◽  
Author(s):  
Silvia Corinti ◽  
Raffaele De Palma ◽  
Angelo Fontana ◽  
Maria Cristina Gagliardi ◽  
Carlo Pini ◽  
...  
Keyword(s):  
T Cells ◽  
Major Histocompatibility Complex ◽  
T Cell ◽  
Cd8 T Cells ◽  
T Cell Response ◽  
Major Histocompatibility ◽  
T Cell Clones ◽  
Histocompatibility Complex ◽  
Cell Clones

We have isolated CD8+ α/β T cells from the blood of atopic and healthy individuals which recognize a nonpeptide antigen present in an allergenic extract from Parietaria judaica pollen. This antigen appears to be a carbohydrate because it is resistant to proteinase K and alkaline digestion, is hydrophilic, and is sensitive to trifluoromethane-sulphonic and periodic acids. In addition, on a reverse-phase high performance liquid chromatography column the antigen recognized by CD8+ T cells separates in a fraction which contains >80% hexoses (glucose and galactose) and undetectable amounts of proteins. Presentation of this putative carbohydrate antigen (PjCHOAg) to CD8+ T cell clones is dependent on live antigen presenting cells (APCs) pulsed for >1 h at 37°C, suggesting that the antigen has to be internalized and possibly processed. Indeed, fixed APCs or APCs pulsed at 15°C were both unable to induce T cell response. Remarkably, PjCHOAg presentation is independent of the expression of classical major histocompatibility complex (MHC) molecules or CD1. CD8+ T cells stimulated by PjCHOAg-pulsed APCs undergo a sustained [Ca2+]i increase and downregulate their T cell antigen receptors (TCRs) in an antigen dose– and time-dependent fashion, similar to T cells stimulated by conventional ligands. Analysis of TCR Vβ transcripts shows that six independent PjCHOAg-specific T cell clones carry the Vβ8 segment with a conserved motif in the CDR3 region, indicating a structural requirement for recognition of this antigen. Finally, after activation, the CD8+ clones from the atopic patient express CD40L and produce high levels of interleukins 4 and 5, suggesting that the clones may have undergone a Th2-like polarization in vivo. These results reveal a new class of antigens which triggers T cells in an MHC-independent way, and these antigens appear to be carbohydrates. We suggest that this type of antigen may play a role in the immune response in vivo.

scholarly journals Analysis of cross-reactive antigen-specific T cell clones. Specific recognition of two major histocompatibility complex (MHC) and two non-MHC antigens by a single clone.

1983 ◽  
Vol 157 (6) ◽  
pp. 2147-2153 ◽  
Author(s):  
A Ben-Nun ◽  
Z Lando ◽  
M E Dorf ◽  
S J Burakoff
Keyword(s):  
Major Histocompatibility Complex ◽  
T Cell ◽  
Basic Protein ◽  
Major Histocompatibility ◽  
T Cell Clones ◽  
Mhc Antigens ◽  
Histocompatibility Complex ◽  
Cell Clones ◽  
Single Clone ◽  
Specific Association

Two T cell clones, one specific for I-Es/d plus myelin basic protein (BP) and another specific for I-Ak plus influenza virus have been demonstrated to cross-react with DBA/2 cells. Genetic and serological analyses have shown that each clone recognizes its respective priming antigen in association with self-major histocompatibility complex (MHC) determinants and each recognizes DBA/2 minor H antigens in association with allo I-Ad MHC antigens. Further analysis of these clones suggests (a) that the allo I-Ad MHC epitopes recognized by these clones are not shared with self-I-A epitopes, (b) that the virus or BP antigens do not cross-react with DBA/2 minor H antigens, (c) that these clones recognize different determinants on the DBA/2 minor H antigens, and (d) that there is a requirement for a specific association between the different MHC antigens and the non-MHC antigens to stimulate these clones. This specific associative recognition argues strongly for the "altered self" hypothesis.

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