Stimulation of monoclonal antibody production by human-human hybridoma cells with an elevated concentration of potassium or sodium phosphate in serum-free medium

1989 ◽  
Vol 2 (1) ◽  
pp. 63-67 ◽  
Author(s):  
S. Sato ◽  
H. Murakami ◽  
T. Sugahara ◽  
T. Ikegami ◽  
K. Yamada ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Antibody Production ◽  
Sodium Phosphate ◽  
Hybridoma Cells ◽  
Elevated Concentration ◽  
Serum Free Medium ◽  
Free Medium ◽  
Monoclonal Antibody Production ◽  
Serum Free ◽  
Stimulation Of

scholarly journals Stimulierung von Kulturen embryonaler Rattenzellen durch eine Proteinfraktion aus fötalem Kälberserum / Stimulation of Embryonic Rat Cells in Culture by a Protein Fraction Isolated from Fetal Calf Serum

1971 ◽  
Vol 26 (10) ◽  
pp. 1045-1048 ◽  
Author(s):  
Dieter F. Hülser ◽  
Werner Frank
Keyword(s):  
Cell Cycle ◽  
Culture Medium ◽  
Fetal Calf Serum ◽  
Surface Membrane ◽  
Calf Serum ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Membrane Potential Difference ◽  
Stimulation Of

Normal embryonic rat cells incubated in serum-free medium accumulate in G1-phase of the cell cycle. On addition of a growth-stimulating protein isolated from fetal calf serum they are triggered to proceed through the cycle, and they resume DNA-synthesis 15 to 20 hours later. In this paper it is demonstrated that the surface membrane potential difference (PD) decreases immediately after changing serum-free medium against culture medium containing either calf serum or the isolated serum protein; the original PD is restored 2 to 3 hours later. Serumprotein without growthstimulating activity does not affect the PD.A permanent rat cell line which grows independently of serum also has been tested. The PD of these cells is not significantly influenced by calf serum.

Comparison of commercial serum-free media for CHO-K1 cell growth and monoclonal antibody production

2012 ◽  
Vol 437 (1-2) ◽  
pp. 303-305 ◽  
Author(s):  
Maria Elisa Rodrigues ◽  
Ana Rita Costa ◽  
Mariana Henriques ◽  
Joana Azeredo ◽  
Rosário Oliveira
Keyword(s):  
Monoclonal Antibody ◽  
Cell Growth ◽  
Antibody Production ◽  
Monoclonal Antibody Production ◽  
Serum Free ◽  
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