Eimeria tenella: Inhibition of development in cell culture by serum from chickens fed anticoccidial drugs

1977 ◽  
Vol 54 (1) ◽  
pp. 95-100 ◽  
Author(s):  
L. R. McDougald ◽  
R. B. Galloway
Keyword(s):  
Cell Culture ◽  
Eimeria Tenella

Sexual differentiation of merozoites of Eimeria tenella

Parasitology ◽  
1972 ◽  
Vol 65 (1) ◽  
pp. 131-136 ◽  
Author(s):  
B. Klimes ◽  
D. G. Rootes ◽  
Zabel Tanielian
Keyword(s):  
Cell Culture ◽  
Sex Determination ◽  
Kidney Cell ◽  
Sexual Differentiation ◽  
Eimeria Tenella ◽  
Kidney Cell Culture ◽  
Pas Staining

In chickens kidney-cell culture gametogony of E. tenella usually occurs in a limited number of selected cells in the form of nests of gametocytes. Some cells contain only macrogametocytes, others only microgametocytes and some cells contain both sexes together. Mixed nests of gametocytes are evidence for genetic sex determination. By using PAS staining the origin of macrogametocytes and microgametocytes can be retraced to the merozoites and schizonts of the last generation, which are differentiated by this technique.

scholarly journals The Growth of Eimeria tenella: Characterization and Application of Quantitative Methods to Assess Sporozoite Invasion and Endogenous Development in Cell Culture

2020 ◽  
Vol 10 ◽  
Author(s):  
Virginia Marugan-Hernandez ◽  
Georgia Jeremiah ◽  
Kelsilandia Aguiar-Martins ◽  
Alana Burrell ◽  
Sue Vaughan ◽  
...  
Keyword(s):  
Cell Culture ◽  
Quantitative Methods ◽  
Eimeria Tenella ◽  
Endogenous Development

Adapting Eimeria tenella to Grow in Primary Chicken Kidney Cells Following Repeated Passages between Cell Culture and Chickens

1997 ◽  
Vol 41 (1) ◽  
pp. 111 ◽  
Author(s):  
Jianfei Zhang ◽  
Eric Wilson ◽  
Shiguang Yang ◽  
Mark C. Healey
Keyword(s):  
Cell Culture ◽  
Eimeria Tenella ◽  
Kidney Cells ◽  
Chicken Kidney

Eimeria tenella: Temperature and asexual development in cell culture

1969 ◽  
Vol 25 ◽  
pp. 324-328 ◽  
Author(s):  
R.G. Strout ◽  
C.A. Ouellette ◽  
D.P. Gangi
Keyword(s):  
Cell Culture ◽  
Eimeria Tenella ◽  
Asexual Development

Laboratory studies with some older anticoccidials

Parasitology ◽  
1976 ◽  
Vol 73 (3) ◽  
pp. 287-309 ◽  
Author(s):  
J. F. Ryley ◽  
R. G. Wilson
Keyword(s):  
Cell Culture ◽  
Life Cycle ◽  
Eimeria Tenella ◽  
Laboratory Studies ◽  
Tissue Destruction ◽  
2Nd Generation ◽  
Medication Effects ◽  
Anticoccidial Activity

Features of the anticoccidial activity of nicarbazin, amprolium, zoalene, sulphadimidine, diaveridine, Darvisul, spiramycin, chloramphenicol and Oxytetracycline have been re-investigated both in vivo and in cell culture using Eimeria tenella. Of the drugs studied, only spiramycin was appreciably coccidiocidal, although nicarbazin and amprolium showed possibly slower coccidiocidal activity. In order to show activity against a particular stage in the life-cycle, higher concentrations of drug than those usually recommended for field usage had in most cases to be used. Under these conditions, parasites were usually inhibited as multinucleate 1st generation schizonts. With delayed medication, effects against 2nd generation parasites were in most cases found, and in many cases, although the parasites never matured to give viable merozoites, the large degenerating forms produced were able to cause extensive tissue destruction and haemorrhage. Methodology in this type of study is discussed in relation to more active and more recent anticoccidials, and some further experiments with robenidine reported.

Sign in / Sign up

Export Citation Format

Share Document