scholarly journals The Growth of Eimeria tenella: Characterization and Application of Quantitative Methods to Assess Sporozoite Invasion and Endogenous Development in Cell Culture

2020 ◽  
Vol 10 ◽  
Author(s):  
Virginia Marugan-Hernandez ◽  
Georgia Jeremiah ◽  
Kelsilandia Aguiar-Martins ◽  
Alana Burrell ◽  
Sue Vaughan ◽  
...  
Keyword(s):  
Cell Culture ◽  
Quantitative Methods ◽  
Eimeria Tenella ◽  
Endogenous Development

Sexual differentiation of merozoites of Eimeria tenella

Parasitology ◽  
1972 ◽  
Vol 65 (1) ◽  
pp. 131-136 ◽  
Author(s):  
B. Klimes ◽  
D. G. Rootes ◽  
Zabel Tanielian
Keyword(s):  
Cell Culture ◽  
Sex Determination ◽  
Kidney Cell ◽  
Sexual Differentiation ◽  
Eimeria Tenella ◽  
Kidney Cell Culture ◽  
Pas Staining

In chickens kidney-cell culture gametogony of E. tenella usually occurs in a limited number of selected cells in the form of nests of gametocytes. Some cells contain only macrogametocytes, others only microgametocytes and some cells contain both sexes together. Mixed nests of gametocytes are evidence for genetic sex determination. By using PAS staining the origin of macrogametocytes and microgametocytes can be retraced to the merozoites and schizonts of the last generation, which are differentiated by this technique.

Adapting Eimeria tenella to Grow in Primary Chicken Kidney Cells Following Repeated Passages between Cell Culture and Chickens

1997 ◽  
Vol 41 (1) ◽  
pp. 111 ◽  
Author(s):  
Jianfei Zhang ◽  
Eric Wilson ◽  
Shiguang Yang ◽  
Mark C. Healey
Keyword(s):  
Cell Culture ◽  
Eimeria Tenella ◽  
Kidney Cells ◽  
Chicken Kidney

Novel Quantitative Methods for the Determination of Biomaterial Cytotoxicity

1992 ◽  
Vol 15 (3) ◽  
pp. 191-194 ◽  
Author(s):  
M.D. Smith ◽  
J.C. Barbenel ◽  
J.M. Courtney ◽  
M.H. Grant
Keyword(s):  
Cell Culture ◽  
Medical Devices ◽  
Mtt Assay ◽  
Quantitative Methods ◽  
Artificial Organs ◽  
Fluorimetric Assay ◽  
Standardized Protocol ◽  
Ht1080 Cells ◽  
Quantitative Results

Two novel methods for the determination of biomaterial cytotoxicity using cell culture are presented. The methods combine a standardized protocol for producing extracts from medical devices with either the established MTT assay or a new fluorimetric assay. The suitability of both methods for evaluating the toxicity of candidate materials was demonstrated by resolution of the differences in the toxic effects of serial dilutions of a PVC extract on BHK21 and HT1080 cells. The tests yield highly reproducible, quantitative results and can be applied to materials in the usual physical forms applicable to artificial organs.

Sign in / Sign up

Export Citation Format

Share Document