Control of Herpes simplex virus thymidine kinase gene expression in Saccharomyces cerevisiae by a yeast promoter sequence

1984 ◽  
Vol 194 (1-2) ◽  
pp. 31-41 ◽  
Author(s):  
Xin-Liang Zhu ◽  
Cheryl Ward ◽  
Arthur Weissbach
Keyword(s):  
Gene Expression ◽  
Saccharomyces Cerevisiae ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Thymidine Kinase ◽  
Promoter Sequence ◽  
Thymidine Kinase Gene ◽  
Kinase Gene ◽  
Simplex Virus

18F-FEAU as a radiotracer for herpes simplex virus thymidine kinase gene expression: in-vitro comparison with other PET tracers

2006 ◽  
Vol 27 (1) ◽  
pp. 25-30 ◽  
Author(s):  
Anne Rixt Buursma ◽  
Vera Rutgers ◽  
Geke A.P. Hospers ◽  
Nanno H. Mulder ◽  
Willem Vaalburg ◽  
...  
Keyword(s):  
Gene Expression ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Thymidine Kinase ◽  
Thymidine Kinase Gene ◽  
Kinase Gene ◽  
Pet Tracers ◽  
Simplex Virus ◽  
In Vitro Comparison

scholarly journals The herpes simplex virus thymidine kinase gene is not transcribed in Saccharomyces cerevisiae.

1982 ◽  
Vol 149 (2) ◽  
pp. 542-547 ◽  
Author(s):  
G B Kiss ◽  
R E Pearlman ◽  
K V Cornish ◽  
J D Friesen ◽  
V L Chan
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Thymidine Kinase ◽  
Thymidine Kinase Gene ◽  
Kinase Gene ◽  
Simplex Virus

Comparison of [18F]FHPG and [124/125I]FIAU for imaging herpes simplex virus type 1 thymidine kinase gene expression

2001 ◽  
Vol 28 (6) ◽  
pp. 721-729 ◽  
Author(s):  
Peter Brust ◽  
Roland Haubner ◽  
Anne Friedrich ◽  
Matthias Scheunemann ◽  
Martina Anton ◽  
...  
Keyword(s):  
Gene Expression ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Thymidine Kinase ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
Thymidine Kinase Gene ◽  
Kinase Gene ◽  
Simplex Virus

SPECT Imaging of Herpes Simplex Virus Type1 Thymidine Kinase Gene Expression By [123I]FIAU1

2005 ◽  
Vol 12 (7) ◽  
pp. 798-805 ◽  
Author(s):  
Seok Rye Choi ◽  
Zhi-Ping Zhuang ◽  
Ann-Marie Chacko ◽  
Paul D. Acton ◽  
Juri Tjuvajev-Gelovani ◽  
...  
Keyword(s):  
Gene Expression ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Thymidine Kinase ◽  
Spect Imaging ◽  
Thymidine Kinase Gene ◽  
Kinase Gene ◽  
Simplex Virus

scholarly journals Binding of hnRNP L to the Pre-mRNA Processing Enhancer of the Herpes Simplex Virus Thymidine Kinase Gene Enhances both Polyadenylation and Nucleocytoplasmic Export of Intronless mRNAs

2005 ◽  
Vol 25 (15) ◽  
pp. 6303-6313 ◽  
Author(s):  
Shouhong Guang ◽  
Alicia M. Felthauser ◽  
Janet E. Mertz
Keyword(s):  
Gene Expression ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Thymidine Kinase ◽  
Globin Gene ◽  
Mrna Processing ◽  
Thymidine Kinase Gene ◽  
Kinase Gene ◽  
Simplex Virus ◽  
Nucleocytoplasmic Export

ABSTRACT Liu and Mertz (Genes Dev. 9:1766-1780, 1995) previously identified a 119-nt pre-mRNA processing enhancer (PPE) element within the herpes simplex virus type 1 thymidine kinase gene that enables intron-independent gene expression in higher eukaryotes by binding heterogeneous nuclear ribonucleoprotein L (hnRNP L). Here, we identify a 49-nt subelement within this PPE that enhanced stability, polyadenylation, and cytoplasmic accumulation of transcripts synthesized in CV-1 cells from an intronless variant of the human β-globin gene when present in two or more tandem copies. This 2×TK49 PPE also enhanced (i) the efficiency of polyadenylation of intronless β-globin RNA in a cell-free polyadenylation system and (ii) the kinetics of nucleocytoplasmic export of an intronless variant of adenovirus major late leader region RNA in Xenopus oocytes. This 2×TK49 PPE bound only hnRNP L. Analysis of 2×TK49 PPE mutants showed a strong positive correlation existed between binding hnRNP L and enhancement of intronless β-globin gene expression. hnRNP L was found to associate with both the mRNA export factor TAP and the exon-exon junction complex protein Aly/REF. Thus, we conclude that hnRNP L plays roles in enhancing stability, polyadenylation, and nucleocytoplasmic export; it does so, at least in part, by directly recruiting to intronless PPE-containing RNAs cofactors normally recruited to intron-containing RNAs.

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