Cloning of colicin E1 tolerant tolC(mtcB) gene of Escherichia coli K12 and identification of its gene product

1982 ◽  
Vol 187 (1) ◽  
pp. 30-36 ◽  
Author(s):  
Nozomu Otsuji ◽  
Toshinori Soejima ◽  
Satoko Maki ◽  
Hideo Shinagawa
Keyword(s):  
Escherichia Coli ◽  
Gene Product ◽  
Escherichia Coli K12 ◽  
Colicin E1 ◽  
Its Gene

The recN locus of Escherichia coli K12: molecular analysis and identification of the gene product

1985 ◽  
Vol 201 (2) ◽  
pp. 301-307 ◽  
Author(s):  
Steven M. Picksley ◽  
Stuart J. Morton ◽  
Robert G. Lloyd
Keyword(s):  
Escherichia Coli ◽  
Gene Product ◽  
Molecular Analysis ◽  
Escherichia Coli K12

scholarly journals The Pyrimidine Nucleotide Reductase Step in Riboflavin and F420 Biosynthesis in Archaea Proceeds by the Eukaryotic Route to Riboflavin

2002 ◽  
Vol 184 (7) ◽  
pp. 1952-1957 ◽  
Author(s):  
Marion Graupner ◽  
Huimin Xu ◽  
Robert H. White
Keyword(s):  
Escherichia Coli ◽  
Gene Product ◽  
Pyridine Nucleotide ◽  
The Other ◽  
Riboflavin Biosynthesis ◽  
Methanosarcina Thermophila ◽  
Pyrimidine Nucleotide ◽  
Cell Extracts ◽  
Phosphate Compound ◽  
Its Gene

ABSTRACT The Methanococcus jannaschii gene MJ0671 was cloned and overexpressed in Escherichia coli, and its gene product was tested for its ability to catalyze the pyridine nucleotide-dependent reduction of either 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5′-phosphate (compound 3) to 2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5′-phosphate (compound 4) or 5-amino-6-ribosylamino-2,4(1H,3H)-pyrimidinedione 5′-phosphate (compound 7) to 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5′-phosphate (compound 5). Only compound 3 was found to serve as a substrate for the enzyme. NADPH and NADH functioned equally well as the reductants. This specificity for the reduction of compound 3 was also confirmed by using cell extracts of M. jannaschii and Methanosarcina thermophila. Thus, this step in riboflavin biosynthesis in these archaea is the same as that found in yeasts. The absence of the other genes in the biosynthesis of riboflavin in Archaea is discussed.

Purification of the NusB gene product of Escherichia coli K12

1985 ◽  
Vol 200 (1) ◽  
pp. 14-20 ◽  
Author(s):  
Toshio Maekawa ◽  
Takahiro Nagase ◽  
Fumio Imamoto ◽  
Shunsuke Ishii
Keyword(s):  
Escherichia Coli ◽  
Gene Product ◽  
Escherichia Coli K12

Purification and properties of the Endo-1,4-β-glucanase from Ruminococcus albus and its gene product in Escherichia coli

1991 ◽  
Vol 71 (4) ◽  
pp. 221-225 ◽  
Author(s):  
Hiroyuki Deguchi ◽  
Yuichi Watanabe ◽  
Takuji Sasaki ◽  
Tsukasa Matsuda ◽  
Shoichi Shimizu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gene Product ◽  
Ruminococcus Albus ◽  
Purification And Properties ◽  
Its Gene
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