Localization and characterization of recombinant DNA clones derived from the highly repetitive DNA sequences in the Indian muntjac cells: their presence in the Chinese muntjac

Chromosoma ◽  
1986 ◽  
Vol 93 (6) ◽  
pp. 521-528 ◽  
Author(s):  
Loh-Chung Yu ◽  
Daniel Lowensteiner ◽  
Elsie F. -K. Wong ◽  
Ikuhisa Sawada ◽  
Joe Mazrimas ◽  
...  
1986 ◽  
Vol 72 (2) ◽  
pp. 207-210 ◽  
Author(s):  
M. Metzlaff ◽  
W. Troebner ◽  
F. Baldauf ◽  
R. Schlegel ◽  
J. Cullum

Genome ◽  
1995 ◽  
Vol 38 (6) ◽  
pp. 1061-1069 ◽  
Author(s):  
A. Cuadrado ◽  
N. Jouve ◽  
C. Ceoloni

The molecular characterization of heterochromatin in six lines of rye has been performed using fluorescence in situ hybridization (FISH). The highly repetitive rye DNA sequences pSc 119.2, pSc74, and pSc34, and the probes pTa71 and pSc794 containing the 25S–5.8S–18S rDNA (NOR) and the 5S rDNA multigene families, respectively, were used. This allowed the individual identification of all seven rye chromosomes and most chromosome arms in all lines. All varieties showed similar but not identical patterns. A standard in situ hybridization map was constructed following the nomenclature system recommended for C-bands. All FISH sites observed appeared to correspond well with C-band locations, but not all C-banding sites coincided with hybridization sites of the repetitive DNA probes used. Quantitative and qualitative differences between different varieties were found for in situ hybridization response at corresponding sites. Variation between plants and even between homologous chromosomes of the same plant was found in open-pollinated lines. In inbred lines, the in situ pattern of the homologues was practically identical and no variation between plants was detected. The observed quantitative and qualitative differences are consistent with a corresponding variation for C-bands detected both within and between cultivars.Key words: fluorescence in situ hybridization, repetitive DNA, rye, Secale cereale, polymorphism.


1990 ◽  
Vol 141 (9) ◽  
pp. 1103-1116 ◽  
Author(s):  
E. Gilson ◽  
S. Bachellier ◽  
S. Perrin ◽  
D. Perrin ◽  
P.A.D. Grimont ◽  
...  

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