22 Characterization of highly differentiated rat renal kidney proximal tubule cells in primary cultures: the use of hormonally defined culture media without growth factors

1996 ◽  
Vol 12 (4-6) ◽  
pp. 377-377
Author(s):  
S. Marouillat ◽  
C. Leclere ◽  
C. Monteil ◽  
J. P. Fillastre ◽  
J. P. Morin
Keyword(s):  
Growth Factors ◽  
Proximal Tubule ◽  
Culture Media ◽  
Primary Cultures ◽  
Proximal Tubule Cells ◽  
Kidney Proximal Tubule ◽  
Tubule Cells ◽  
Defined Culture

Nephrotoxicity Testing In Vitro: The Current Situation

1997 ◽  
Vol 25 (5) ◽  
pp. 497-503
Author(s):  
Jean-Paul Morin ◽  
Marc E. De Broe ◽  
Walter Pfaller ◽  
Gabriele Schmuck
Keyword(s):  
Proximal Tubule ◽  
Culture Media ◽  
Task Force ◽  
Primary Cultures ◽  
Phenotypic Expression ◽  
Transepithelial Transport ◽  
Specific Cell ◽  
Proximal Tubule Cells ◽  
Tubule Cells

An ECVAM task force on nephrotoxicity has been established to advise, in particular, on the follow-up to recommendations made in the ECVAM workshop report on nephrotoxicity testing in vitro. Since this workshop was held, in 1994, there have been several improvements in the techniques used. For example, the duration of renal slice viability, and the maintenance of functional activities in slices, have been improved by using dynamic incubation systems with higher oxygen tensions and more-appropriate cell culture media. Highly differentiated primary cultures of pig, human and rabbit proximal tubule cells have been established by using specific cell isolation procedures and/or selective culture media. To date, the most comparable phenotypic expression and transepithelial transport capacities to proximal tubules in vivo have been obtained with primary cultures of rabbit proximal tubule cells which are grown on bicompartmental supports; in this system, transepithelial substrate gradients are generated and the transepithelial transport of both organic anions and cations is highly active. This in vitro system has been selected by ECVAM for further evaluation and prevalidation. Industrial needs in the area of nephrotoxicity testing have been identified, and recommendations are made at the end of this report concerning possible future initiatives.

Characterization of Gentamicin-Induced DysfunctionsIn Vitro: The Use of Optimized Primary Cultures of Rabbit Proximal Tubule Cells

Renal Failure ◽  
1993 ◽  
Vol 15 (4) ◽  
pp. 475-483 ◽  
Author(s):  
Christelle Monteil ◽  
Caroline Leclere ◽  
Jean Paul Fillastre ◽  
Jean Paul Morin
Keyword(s):  
Proximal Tubule ◽  
Primary Cultures ◽  
Proximal Tubule Cells ◽  
Tubule Cells

Primary cultures of renal proximal tubule cells derived from individuals with primary hyperoxaluria

2009 ◽  
Vol 37 (3) ◽  
pp. 127-132 ◽  
Author(s):  
Karen L. Price ◽  
Sally-Anne Hulton ◽  
William G. van’t Hoff ◽  
John R. Masters ◽  
Gill Rumsby
Keyword(s):  
Proximal Tubule ◽  
Primary Cultures ◽  
Primary Hyperoxaluria ◽  
Renal Proximal Tubule ◽  
Proximal Tubule Cells ◽  
Tubule Cells ◽  
Renal Proximal Tubule Cells
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