Improved localization of thiamine pyrophosphatase activity in mounted cryostat sections using gel fixation and gel incubation media

1978 ◽  
Vol 56 (3-4) ◽  
pp. 345-347 ◽  
Author(s):  
Zdeněk Lukáš ◽  
Karel Dvořák ◽  
Zdeňka Juránková
Keyword(s):  
Thiamine Pyrophosphatase ◽  
Pyrophosphatase Activity ◽  
Cryostat Sections

Intracellular digestion and cellular defecation in a monogenean, Diclidophora merlangi

Parasitology ◽  
1975 ◽  
Vol 70 (3) ◽  
pp. 331-340 ◽  
Author(s):  
D. W. Halton
Keyword(s):  
Endoplasmic Reticulum ◽  
Golgi Apparatus ◽  
Digestive Enzymes ◽  
Host Protein ◽  
Blood Proteins ◽  
Host Fish ◽  
Intracellular Digestion ◽  
Thiamine Pyrophosphatase ◽  
Lysosomal System ◽  
Pyrophosphatase Activity

The ultrastructural and cytochemical changes accompanying intracellular digestion and cellular defecation in the haematin cell of Diclidophora merlangi have been described. Blood proteins of the host-fish are sequestered by endocytosis and degraded within an interconnecting network of channels that form an integral, but changing, part of the cell. The digestive enzymes involved originate in the granular endoplasmic reticulum and are packaged in the Golgi apparatus and transferred to the channels in Golgi vesicles. The rate of haemoglobin absorption and the activity of the Golgi, as judged by vesicle counts and staining intensities for thiamine pyrophosphatase activity, are stimulated by the introduction of host protein into the gut lumen. The haematin residues of digestion are extruded periodically into the lumen by exocytosis involving membrane fusion. The process is a continuous one and, in worms starved of food, can result in the complete evacuation of pigment from the cell. It is suggested that a lysosomal system operates in the digestive cycle of the haematin cell.

Ultrastructural localization of thiamine pyrophosphatase activity in the intestinal epithelial cells of adult rats

1984 ◽  
Vol 74 (2) ◽  
pp. 181-187 ◽  
Author(s):  
Yukio Oomori ◽  
Kazuyuki Ono ◽  
Katsushi Ishikawa ◽  
Yoh-ich Satoh ◽  
Mitsuaki Matoba
Keyword(s):  
Epithelial Cells ◽  
Intestinal Epithelial Cells ◽  
Ultrastructural Localization ◽  
Intestinal Epithelial ◽  
Adult Rats ◽  
Thiamine Pyrophosphatase ◽  
Pyrophosphatase Activity

Histochemical evaluation of thiamine pyrophosphatase activity during first molar odontogenesis of the neonatal hairless mouse

1977 ◽  
Vol 60 (1) ◽  
pp. 78-90 ◽  
Author(s):  
Brian L. Kuyatt ◽  
Leslie P. Gartner ◽  
James L. Hiatt ◽  
D. Vincent Provenza
Keyword(s):  
Hairless Mouse ◽  
Thiamine Pyrophosphatase ◽  
Pyrophosphatase Activity

scholarly journals AN ULTRACYTOCHEMICAL AND ULTRASTRUCTURAL STUDY OF EPITHELIAL CELLS IN DUCTULI EFFERENTES OF CHINESE HAMSTER

1971 ◽  
Vol 19 (12) ◽  
pp. 766-774 ◽  
Author(s):  
MASAO YOKOYAMA ◽  
JEFFREY P. CHANG
Keyword(s):  
Epithelial Cells ◽  
Golgi Apparatus ◽  
Phosphatase Activity ◽  
Plasma Membranes ◽  
Chinese Hamster ◽  
Fluid Composition ◽  
Ciliated Cells ◽  
Efferent Duct ◽  
Thiamine Pyrophosphatase ◽  
Pyrophosphatase Activity

Ultrastructural and ultracytochemical features of the efferent duct of Chinese hamster have been studied. The ducts are composed of two main types of epithelial cells, ciliated and nonciliated. Distinct structural and cytochemical characteristics of these cells are apparent. Presence of fibrogranular complex which is supposedly related to basal body replication was demonstrated in ciliated cells for the first time in this tissue. Thiamine pyrophosphatase activity in Golgi apparatus, acid phosphatase activity in Golgi apparatus and lysosomes and alkaline phosphatase activity on basal plasma membranes of both ciliated and nonciliated cells have been localized. However, thiamine pyrophosphatase activity was seen only on the luminal surface, apical vacuole and apical tubular structure of nonciliated cells but not on the surface of ciliated cells. Similarly, horseradish peroxidase was absorbed only by nonciliated cells. The cytochemical and ultrastructural differences between the two types of cells indicate a functional specialization. The results indicate that the ciliated cells are concerned with the transportation of the sperms and that the nonciliated cells are concerned with the regulation of fluid composition in the duct since the latter are capable of both secretion and absorption.

scholarly journals ELECTRON MICROSCOPIC LOCALIZATION OF ACID PHOSPHATASE AND THIAMINE PYROPHOSPHATASE ACTIVITY IN HYPOTHALAMIC NEUROSECRETORY CELLS OF THE RAT

1964 ◽  
Vol 21 (1) ◽  
pp. 35-47 ◽  
Author(s):  
Joseph Osinchak
Keyword(s):  
Acid Phosphatase ◽  
Osmium Tetroxide ◽  
Neurosecretory Cells ◽  
Electron Microscopic ◽  
Vascular Perfusion ◽  
Dense Bodies ◽  
Thiamine Pyrophosphatase ◽  
Pyrophosphatase Activity ◽  
Supraoptic Nuclei ◽  
Electron Microscopic Localization

Supraoptic nuclei in the hypothalamus of rats were fixed for the electron microscope by vascular perfusion with solutions of glutaraldehyde followed by post fixation with osmium tetroxide. Cytochemical methods for detection of acid phosphatase and thiamine pyrophosphatase activity have been applied to glutaraldehyde-fixed frozen sections containing the neurosecretory cells. The enzyme activities have been localized to certain Golgi cisternae. Acid phosphatase activity is present in the large (0.4 µ to 1.0 µ) granules or dense bodies which are surrounded by a single limiting membrane; both features characterize these structures as lysosomes. Smaller (0.1 µ) granules also present in the perikarya are generally unreactive towards enzyme activity and resemble in form the neurosecretory granules in the neurohypophysis.

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