Ultrastructural localization of thiamine pyrophosphatase activity in epitheliocytes in duodenal biopsy material in peptic ulcer

1987 ◽  
Vol 103 (6) ◽  
pp. 856-860
Author(s):  
G. V. Panasyuk ◽  
G. I. Nepomnyashchikh ◽  
M. G. Chernokalova ◽  
L. M. Nepomnyashchikh
Keyword(s):  
Peptic Ulcer ◽  
Ultrastructural Localization ◽  
Duodenal Biopsy ◽  
Biopsy Material ◽  
Thiamine Pyrophosphatase ◽  
Pyrophosphatase Activity

Ultrastructural localization of thiamine pyrophosphatase activity in the intestinal epithelial cells of adult rats

1984 ◽  
Vol 74 (2) ◽  
pp. 181-187 ◽  
Author(s):  
Yukio Oomori ◽  
Kazuyuki Ono ◽  
Katsushi Ishikawa ◽  
Yoh-ich Satoh ◽  
Mitsuaki Matoba
Keyword(s):  
Epithelial Cells ◽  
Intestinal Epithelial Cells ◽  
Ultrastructural Localization ◽  
Intestinal Epithelial ◽  
Adult Rats ◽  
Thiamine Pyrophosphatase ◽  
Pyrophosphatase Activity

Improved localization of thiamine pyrophosphatase activity in mounted cryostat sections using gel fixation and gel incubation media

1978 ◽  
Vol 56 (3-4) ◽  
pp. 345-347 ◽  
Author(s):  
Zdeněk Lukáš ◽  
Karel Dvořák ◽  
Zdeňka Juránková
Keyword(s):  
Thiamine Pyrophosphatase ◽  
Pyrophosphatase Activity ◽  
Cryostat Sections

Intracellular digestion and cellular defecation in a monogenean, Diclidophora merlangi

Parasitology ◽  
1975 ◽  
Vol 70 (3) ◽  
pp. 331-340 ◽  
Author(s):  
D. W. Halton
Keyword(s):  
Endoplasmic Reticulum ◽  
Golgi Apparatus ◽  
Digestive Enzymes ◽  
Host Protein ◽  
Blood Proteins ◽  
Host Fish ◽  
Intracellular Digestion ◽  
Thiamine Pyrophosphatase ◽  
Lysosomal System ◽  
Pyrophosphatase Activity

The ultrastructural and cytochemical changes accompanying intracellular digestion and cellular defecation in the haematin cell of Diclidophora merlangi have been described. Blood proteins of the host-fish are sequestered by endocytosis and degraded within an interconnecting network of channels that form an integral, but changing, part of the cell. The digestive enzymes involved originate in the granular endoplasmic reticulum and are packaged in the Golgi apparatus and transferred to the channels in Golgi vesicles. The rate of haemoglobin absorption and the activity of the Golgi, as judged by vesicle counts and staining intensities for thiamine pyrophosphatase activity, are stimulated by the introduction of host protein into the gut lumen. The haematin residues of digestion are extruded periodically into the lumen by exocytosis involving membrane fusion. The process is a continuous one and, in worms starved of food, can result in the complete evacuation of pigment from the cell. It is suggested that a lysosomal system operates in the digestive cycle of the haematin cell.

scholarly journals Comparative analysis of genotypes of Helicobacter pylori strains in the Rostov and Astrakhan regions

2018 ◽  
Vol 9 (4) ◽  
pp. 81-86
Author(s):  
V. M. Sorokin ◽  
R. V. Pisanov ◽  
A. S Vodop’janov ◽  
E. V. Golubkina ◽  
E. A. Bereznjak
Keyword(s):  
Helicobacter Pylori ◽  
Peptic Ulcer ◽  
Comparative Analysis ◽  
Mucous Membrane ◽  
Adult Population ◽  
Age Groups ◽  
Biopsy Material ◽  
Pathogenicity Factors ◽  
Rostov Region ◽  
H Pylori

Objective:to study the infection of the adult and children’s population of the Rostov and Astrakhan regions by the bacteria Helicobacter pylori, to determine the frequency of H. pylori pathogenicity factors in different age groups of the population.Materials and methods:118 adults and 112 children were examined. Te presence of H. pylori DNA and the pathogenicity factors of CagA and VacA in the biopsy material of the mucous membrane of the antrum of the stomach was determined by PCR.Results:a signifcantly smaller percentage of H. pylori–positive patients is characteristic of the child population. Te prevalent H. pylori genotype in the infant population is the avirulent genotype Vac s2m2 (60%) (χ2: p <0.005). Genotype CagA + VacAs1 VacAm1 is a marker of peptic ulcer for adult population of the Rostov region.Conclusion:Te conducted studies allowed to establish differences in infection of the population with H. pylori bacteria and the frequency of the distribution of virulent genotypes depending on the region, age and severity of the pathology.

Histochemical evaluation of thiamine pyrophosphatase activity during first molar odontogenesis of the neonatal hairless mouse

1977 ◽  
Vol 60 (1) ◽  
pp. 78-90 ◽  
Author(s):  
Brian L. Kuyatt ◽  
Leslie P. Gartner ◽  
James L. Hiatt ◽  
D. Vincent Provenza
Keyword(s):  
Hairless Mouse ◽  
Thiamine Pyrophosphatase ◽  
Pyrophosphatase Activity

scholarly journals AN ULTRACYTOCHEMICAL AND ULTRASTRUCTURAL STUDY OF EPITHELIAL CELLS IN DUCTULI EFFERENTES OF CHINESE HAMSTER

1971 ◽  
Vol 19 (12) ◽  
pp. 766-774 ◽  
Author(s):  
MASAO YOKOYAMA ◽  
JEFFREY P. CHANG
Keyword(s):  
Epithelial Cells ◽  
Golgi Apparatus ◽  
Phosphatase Activity ◽  
Plasma Membranes ◽  
Chinese Hamster ◽  
Fluid Composition ◽  
Ciliated Cells ◽  
Efferent Duct ◽  
Thiamine Pyrophosphatase ◽  
Pyrophosphatase Activity

Ultrastructural and ultracytochemical features of the efferent duct of Chinese hamster have been studied. The ducts are composed of two main types of epithelial cells, ciliated and nonciliated. Distinct structural and cytochemical characteristics of these cells are apparent. Presence of fibrogranular complex which is supposedly related to basal body replication was demonstrated in ciliated cells for the first time in this tissue. Thiamine pyrophosphatase activity in Golgi apparatus, acid phosphatase activity in Golgi apparatus and lysosomes and alkaline phosphatase activity on basal plasma membranes of both ciliated and nonciliated cells have been localized. However, thiamine pyrophosphatase activity was seen only on the luminal surface, apical vacuole and apical tubular structure of nonciliated cells but not on the surface of ciliated cells. Similarly, horseradish peroxidase was absorbed only by nonciliated cells. The cytochemical and ultrastructural differences between the two types of cells indicate a functional specialization. The results indicate that the ciliated cells are concerned with the transportation of the sperms and that the nonciliated cells are concerned with the regulation of fluid composition in the duct since the latter are capable of both secretion and absorption.

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