A new method for membrane reconstitution: Fusion of protein-containing vesicles with planar bilayer membranes below lipid phase transition temperature

1981 ◽  
Vol 7 (3) ◽  
pp. 131-137 ◽  
Author(s):  
W. Hanke ◽  
H. Eibl ◽  
G. Boheim
Keyword(s):  
Phase Transition ◽  
Transition Temperature ◽  
Phase Transition Temperature ◽  
New Method ◽  
Lipid Phase ◽  
Planar Bilayer ◽  
Lipid Phase Transition ◽  
Bilayer Membranes ◽  
Membrane Reconstitution

scholarly journals Protein-mediated lipid transfer. The effects of lipid-phase transition and of charged lipids

1983 ◽  
Vol 213 (1) ◽  
pp. 21-24 ◽  
Author(s):  
Y H Xü ◽  
K Gietzen ◽  
H J Galla ◽  
E Sackmann
Keyword(s):  
Phase Transition ◽  
Transition Temperature ◽  
Phosphatidic Acid ◽  
Phase Transition Temperature ◽  
Fluorescence Polarization ◽  
Exchange Process ◽  
Lipid Phase ◽  
Lipid Transfer ◽  
Lipid Phase Transition ◽  
Polarization Measurements

The protein-mediated phospholipid exchange between small unilamellar vesicles was investigated by fluorescence polarization measurements with diphenylhexatriene as optical probe. Thermotropic phase-transition measurements were taken after mixing two vesicle preparations of distinct and different phase-transition temperatures or having different states of charge. From the heights of each phase-transition step, we were able to follow the lipid-exchange process in the presence, as well as in the absence (natural exchange), of so-called transfer protein isolated from beef liver. A strong enhancement of the lipid transfer was observed at the corresponding lipid-phase-transition temperature, which is explained by the presence of fluctuating fluid and ordered domains co-existing at the lipid-phase-transition temperature. A unidirectional lipid transfer of the neutral component was observed between negatively charged phosphatidic acid and neutral phosphatidylcholine vesicles. Fluorescence polarization measurements showed the disappearance of the phosphatidylcholine phase transition, whereas the phosphatidic acid phase transition broadened and its phase transition temperature became lower.

Formation of supported lipid bilayers on silica: relation to lipid phase transition temperature and liposome size

Soft Matter ◽  
2014 ◽  
Vol 10 (1) ◽  
pp. 187-195 ◽  
Author(s):  
Yujia Jing ◽  
Hana Trefna ◽  
Mikael Persson ◽  
Bengt Kasemo ◽  
Sofia Svedhem
Keyword(s):  
Phase Transition ◽  
Transition Temperature ◽  
Lipid Bilayers ◽  
Phase Transition Temperature ◽  
Supported Lipid Bilayers ◽  
Lipid Phase ◽  
Lipid Phase Transition ◽  
Liposome Size
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