Electrogenic transport of neutral and dibasic amino acids in a cultured opossum kidney cell line (OK)

1989 ◽  
Vol 414 (5) ◽  
pp. 543-550 ◽  
Author(s):  
Johann S. Schwegler ◽  
Almut Heuner ◽  
Stefan Silbernagl
Keyword(s):  
Amino Acids ◽  
Cell Line ◽  
Kidney Cell ◽  
Kidney Cell Line ◽  
Electrogenic Transport ◽  
Opossum Kidney ◽  
Opossum Kidney Cell ◽  
Dibasic Amino Acids

scholarly journals Identification and expression analysis of type II and type III Pi transporters in the opossum kidney cell line

2018 ◽  
Vol 104 (1) ◽  
pp. 149-161 ◽  
Author(s):  
Natalia Guillén ◽  
Yupanqui A. Caldas ◽  
Moshe Levi ◽  
Víctor Sorribas
Keyword(s):  
Cell Line ◽  
Expression Analysis ◽  
Kidney Cell ◽  
Type Ii ◽  
Kidney Cell Line ◽  
Opossum Kidney ◽  
Type Iii ◽  
Opossum Kidney Cell

Structure-activity relationships of parathyroid hormone analogs in the opossum kidney cell line

2009 ◽  
Vol 4 (5) ◽  
pp. 723-730 ◽  
Author(s):  
David L. Carnes ◽  
Judith A. Cole ◽  
Leonard R. Forte ◽  
Richard E. Poelling ◽  
Pamela K. Thorne ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Cell Line ◽  
Kidney Cell ◽  
Kidney Cell Line ◽  
Opossum Kidney ◽  
Structure Activity Relationships ◽  
Structure Activity ◽  
Opossum Kidney Cell ◽  
Hormone Analogs

Insulin binding, internalization, and degradation by a cultured kidney cell line

1988 ◽  
Vol 254 (5) ◽  
pp. E601-E608
Author(s):  
C. Yagil ◽  
U. K. Ehmann ◽  
B. H. Frank ◽  
R. Rabkin
Keyword(s):  
Cell Line ◽  
Kidney Cell ◽  
Insulin Binding ◽  
Insulin Degradation ◽  
Kidney Cell Line ◽  
Insulin Metabolism ◽  
Opossum Kidney ◽  
Membrane Bound ◽  
Hormone Specificity ◽  
Insulin Uptake

Proximal tubules are a key site of insulin metabolism, which is in part a receptor-mediated process. To explore the interaction between insulin and the kidney and to evaluate the role of receptors in insulin uptake and processing, a study was carried out with a cultured proximal-like opossum kidney (OK) cell line. 125I-insulin associated with confluent monolayers in a specific manner, and this interaction was competitively inhibited by insulin; unrelated peptides were relatively ineffective. Insulin degradation exhibited time and temperature dependency and up to a concentration of 5 X 10(-8) M was not saturable. Degradation exhibited partial hormone specificity. Separation of plasma membrane bound from internalized insulin was achieved by lowering extracellular pH. At 4 degrees C, 94% of cell-associated radioactivity was membrane bound, whereas at 37 degrees C, in the steady state, 33% was membrane bound and 67% was internalized. There was a significant correlation between membrane-bound insulin and the rate of degradation. These findings reveal that the binding and processing of insulin by the kidney cell line are compatible with the description of the uptake of filtered insulin by the proximal tubule in the intact kidney. Accordingly we conclude that this cell line provides a good model for studying renal epithelial uptake and metabolism of insulin.

Trials for Preparation of Dog Kidney Cell Line

2014 ◽  
Vol 8 (1) ◽  
pp. 32-38
Author(s):  
Amani Abbas ◽  
Hemmat Alemam ◽  
Hanan El-zawahry
Keyword(s):  
Cell Line ◽  
Kidney Cell ◽  
Kidney Cell Line ◽  
Dog Kidney

scholarly journals A developmentally plastic adult mouse kidney cell line spontaneously generates multiple adult kidney structures

2015 ◽  
Vol 463 (4) ◽  
pp. 1334-1340 ◽  
Author(s):  
Carol F. Webb ◽  
Michelle L. Ratliff ◽  
Rebecca Powell ◽  
Celeste R. Wirsig-Wiechmann ◽  
Olga Lakiza ◽  
...  
Keyword(s):  
Cell Line ◽  
Kidney Cell ◽  
Adult Mouse ◽  
Mouse Kidney ◽  
Kidney Cell Line ◽  
Adult Kidney
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