Association of genetic variants of oxidative stress responsive kinase 1 (OXSR1) with asthma exacerbations in non-smoking asthmatics

Background Asthma exacerbation threatens patient's life. Several genetic studies have been conducted to determine the risk factors for asthma exacerbation, but this information is still lacking. We aimed to determine whether genetic variants of Oxidative Stress Responsive Kinase 1 (OXSR1), a gene with functions of salt transport, immune response, and oxidative stress, are associated with exacerbation of asthma. Methods Clinical data were obtained from 1454 asthmatics and single nucleotide polymorphisms (SNPs) of OXSR1 were genotyped. Genetic associations with annual exacerbation rate were analyzed depending on smoking status. Results Eleven SNPs were selected using Asian data in the International HapMap database. The common allele of rs1384006 C > T of OXSR1 showed a significantly higher annual exacerbation rate than the rare allele in non-smoking asthmatics (CC vs. CT vs. TT: 0.43 ± 0.04 vs. 0.28 ± 0.03 vs. 0.31 ± 0.09, P = 0.004, Pcorr = 0.039). The frequent exacerbators had a significantly higher frequency of the common allele of rs1384006 C > T than did the infrequent exacerbators (74.4% vs. 55.2%, P = 0.004, Pcorr = 0.038). Conclusion The common allele of rs1384006 C > T of OXSR1 was associated with the asthma exacerbation rate and a higher risk of being a frequent exacerbator, indicating that non-smoking asthmatics who carry common alleles may be vulnerable to asthma exacerbations.

On the semi-diagnostic computation of climatological circulation in the western tropical Indian Ocean

The seasonal mean climatological circulation in the Indian Ocean north of 20°S and west of 80°E during the summer and winter has been investigated using a 3-dimensional, fully non-linear, semi-diagnostic circulation model. The model equations include the basic ocean hydrothermodynamic equations of momentum, hydrostatics, continuity, sea surface topography and temperature and salt transport equations. Model is driven with the seasonal mean data on wind stress at the ocean surface and thermohaline forcing at different levels. The circulation in the upper levels of the ocean at 20, 50, 150, 300, 500 and 1000 m depths during the two contrasting seasons has been obtained using the model, and the role of steady, local forcing of wind and internal density field on the dynamical balance of circulation in the western tropical Indian Ocean is explained. The climatological temperature and salinity data used to drive the model is found to be hydrodynamically adjusted with surface wind, flow field and bottom relief during the adaptation stages. Semi-diagnostic technique is found to be very effective for the smoothening of climatic temperature and salinity data and also to obtain the 3-dimensional steady state circulation, which would serve as initial condition in simulation models of circulation.

Barrier Longevity of NaCl-Laden Soil against Subterranean Termites in an Earth Embankment

Subterranean termite-induced damage to earth embankments in agricultural systems occurs globally. NaCl-laden soil barriers (NLSBs) are an environmentally sustainable termite control method, and have exhibited good potential in preventing termite-related tunneling damage in Zhejiang Province, China. The persistence of the NaCl concentration in NLSBs is a key characteristic for the long-term prevention of subterranean termite infestations. This study is a scientific attempt to estimate the field efficacy and barrier longevity of NLSBs in reservoir embankments based on the Richards equation and the convection–dispersion equation using HYDRUS (2D/3D). The observed and simulated NaCl concentrations at the end of a 1915-day simulation were compared. The results indicated that the proposed model performed well and can effectively characterize the water flow and salt transport in NLSBs. The salt desalination rate of the NLSB in the upstream slope was higher than that in the downstream slope, both of which were significantly higher than that at the embankment axis. Regardless of the type of embankment (homogeneous or core-wall), the barrier longevity of NaCl-laden soil against subterranean termites can reach 50 years with an optimized NaCl/soil ratio in different parts of the embankment.

Comparative methylation and RNA-seq expression analysis in CpG context to identify genes involved in Backfat vs. Liver diversification in Nanchukmacdon Pig

Background DNA methylation and demethylation at CpG islands is one of the main regulatory factors that allow cells to respond to different stimuli. These regulatory mechanisms help in developing tissue without affecting the genomic composition or undergoing selection. Liver and backfat play important roles in regulating lipid metabolism and control various pathways involved in reproductive performance, meat quality, and immunity. Genes inside these tissue store a plethora of information and an understanding of these genes is required to enhance tissue characteristics in the future generation. Results A total of 16 CpG islands were identified, and they were involved in differentially methylation regions (DMRs) as well as differentially expressed genes (DEGs) of liver and backfat tissue samples. The genes C7orf50, ACTB and MLC1 in backfat and TNNT3, SIX2, SDK1, CLSTN3, LTBP4, CFAP74, SLC22A23, FOXC1, GMDS, GSC, GATA4, SEMA5A and HOXA5 in the liver, were categorized as differentially-methylated. Subsequently, Motif analysis for DMRs was performed to understand the role of the methylated motif for tissue-specific differentiation. Gene ontology studies revealed association with collagen fibril organization, the Bone Morphogenetic Proteins (BMP) signaling pathway in backfat and cholesterol biosynthesis, bile acid and bile salt transport, and immunity-related pathways in methylated genes expressed in the liver. Conclusions In this study, to understand the role of genes in the differentiation process, we have performed whole-genome bisulfite sequencing (WGBS) and RNA-seq analysis of Nanchukmacdon pigs. Methylation and motif analysis reveals the critical role of CpG islands and transcriptional factors binding site (TFBS) in guiding the differential patterns. Our findings could help in understanding how methylation of certain genes plays an important role and can be used as biomarkers to study tissue specific characteristics.

Variation of water mass exchange on tidal scale in Balikpapan Bay

Balikpapan Bay is enclosed water influenced by freshwater from river runoff and saline water from Makassar Strait. The exchange of water mass was examined by 3D numerical model simulation-Hamburg Shelf Ocean Model (HAMSOM) with horizontal resolutions approx. 150 m and 10 vertical layers applied in Balikpapan Bay. The thirteen tidal components, daily river runoff, atmospheric forcing, subsurface temperature, and Salinity in 3D used for model input. The tidal elevation from Geospatial Information Agency (BIG) model fits with this result from 01/03/2020 to 31/03/2020. It has coefficient correlation 0,99 with a significant level of 95% and Root Mean Square Error (RMSE) is 0,1 m. The volume and salt transport in the mouth (Line-A) and middle (Line-B) of bay was examined. The maximum transport in Line-A during spring (neap) high to low tide and low to high tide is −18364.72 m3/s (−1717.57 m3/s) and −17532.27 m3/s (4258.86 m3/s) for volume. Then, 531,947,898.90 kg.psu./s (−45,127,135.38 kg.psu./s) and −536,410,944.50 kg.psu./s (140,700,437.97 kg.psu./s) for salinity. Positive (negative) of water transport is inflow (outflow) to Balikpapan Bay. The net transport in a day during the spring (neap) is −832.45 m3/s (5976.43 m3/s) for volume and −4,463,045.58 kg.psu./s (185,827,573.35 kg.pau./s) for salt. The vertical structure of net volume and salt transport bot in Line-A and Line-B shows the water goes to outer bay in surface and inner bay in subsurface. While in the spring tide the surface deeper than neap tide. It indicated that water mass exchange dominantly influenced by river in surface and tidal in subsurface. It also shows that water mass from inner bay more easy flushing during spring tide than neap tide and vice versa

Engineering Li/Na selectivity in 12-Crown-4–functionalized polymer membranes

Lithium is widely used in contemporary energy applications, but its isolation from natural reserves is plagued by time-consuming and costly processes. While polymer membranes could, in principle, circumvent these challenges by efficiently extracting lithium from aqueous solutions, they usually exhibit poor ion-specific selectivity. Toward this end, we have incorporated host–guest interactions into a tunable polynorbornene network by copolymerizing 1) 12-crown-4 ligands to impart ion selectivity, 2) poly(ethylene oxide) side chains to control water content, and 3) a crosslinker to form robust solids at room temperature. Single salt transport measurements indicate these materials exhibit unprecedented reverse permeability selectivity (∼2.3) for LiCl over NaCl—the highest documented to date for a dense, water-swollen polymer. As demonstrated by molecular dynamics simulations, this behavior originates from the ability of 12-crown-4 to bind Na+ ions more strongly than Li+ in an aqueous environment, which reduces Na+ mobility (relative to Li+) and offsets the increase in Na+ solubility due to binding with crown ethers. Under mixed salt conditions, 12-crown-4 functionalized membranes showed identical solubility selectivity relative to single salt conditions; however, the permeability and diffusivity selectivity of LiCl over NaCl decreased, presumably due to flux coupling. These results reveal insights for designing advanced membranes with solute-specific selectivity by utilizing host–guest interactions.

Dynamics of salt intrusion in the Mekong Delta: results of field observations and integrated coastal–inland modelling

On the list of challenges facing the world largest deltas, increased saline water intrusion (SWI) in the surface water system and its role in jeopardizing freshwater supply are often ranked very high. Yet, detailed process-based studies of SWI at the whole delta scale are limited, and the trends are regularly associated with global sea level rise. Here, using field measurements and a sophisticated 3D model that integrates the riverine, rural, estuarine, and coastal dynamics within one numerical domain, we study SWI at the scale of the Mekong Delta in extensive detail. While many studies downscale the SWI problem to a topic within an estuary, we show that the physical processes on the continental shelf, such as monsoon-driven ocean surge, directly influence salinity dynamics within the delta. Typical values of 20–40 cm surge over the continental shelf contribute to up to 10 km of further SWI. The delta's estuarine system is also more sensitive than many other systems to variations of river discharge. Furthermore, spring–neap variability plays a key role in SWI in the delta. The estuarine variability from a stratified to a mixed system between neap and spring tides develops 3D processes such as estuarine circulation and tidal straining that become the main upstream salt transport mechanisms. The 3D nature of salinity dynamics, and the role of upstream and downstream processes, suggests that compromising on dimension or extent of the numerical domain can limit the accuracy of predictions of SWI in the delta. The study also showcases the fact that riverbed incision in response to anthropogenic sediment starvation in the last 2 decades has increased stratification and activated or magnified 3D salt transport subprocesses that amplify upstream salt transport. With all the external forces on the delta, namely climate change and an altered hydrological regime by the upstream dams, due to deeper estuarine channels (driven by sand mining and upstream impoundments) compared to its near past, the delta itself has become far more vulnerable to even mild natural events. This exemplifies the fundamental importance of preserving the sediment budget and riverbed levels in protecting the world's deltas against SWI.