Spectrophotometric method for the quantitative determination of benzotef

The oxidative derivatization method using potassium hydrogenperoxomonosulfate for the indirect spectrophotometric determination of Fluphenazine hydrochloride is presented. Potassium hydrogenperoxomonosulfate is introduced as a derivatizing agent for Fluphenazine hydrochloride, yielding the sulfoxide. This reaction product was successfully used for the spectrophotometric determination of the Fluphenazine hydrochloride. The UV spectroscopic detection of the sulfoxide proved to be a more robust and sensitive method. The elaborated method allowed the determination of Fluphenazine hydrochloride in the concentration range of 0.2-30 µg mL-1. The molar absorptivity at 349 nm is 5.6×103 (dm3cm-1mol-1). The limit of quantification, LOQ (10S) is 0.24 µg/mL. A new spectrophotometric technique was developed and the possibility of quantitative determination of Fluphenazine hydrochloride in tablets 5.0 mg was demonstrated. The present method is precise, accurate and excipients did not interfere. RSD for Fluphenazine Hydrochloride 5.0 mg tablets was 1.37 %.

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Quantitative Determination of Benzaldehyde in Flavors and Cordials by Ultraviolet Spectrophotometry and 2,4-Dinitrophenylhydrazine Precipitation

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/50.2.319 ◽

1967 ◽

Vol 50 (2) ◽

pp. 319-322

Author(s):

Richard L Brunelle

Keyword(s):

Quantitative Determination ◽

Spectrophotometric Method ◽

Alternative Methods ◽

Ultraviolet Spectrophotometry ◽

Precipitation Procedure ◽

Gravimetric Procedure

Abstract Three sets of samples were collaboratively analyzed by the official, first action ultraviolet spectrophotometric and 2,4-dinitrophenylhydrazine precipitation methods for determining benzaldehyde in flavors and cordials. The spectrophotometric method was unchanged, except for clarification, and collaborators reported recoveries averaging nearly 100%. The gravimetric procedure using 2,4-dinitrophenylhydrazine gave unsatisfactory recoveries on the first two sets of collaborative samples. However, collaborators reported approximately 96% recovery of benzaldehyde with a modified precipitation procedure on a third set of samples. The ultraviolet spectrophotometric method and the modified 2,4-dinitrophenylhydrazine precipitation procedure are recommended for adoption as official, final action as alternative methods.

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Determination of vitamin A content from selected Nigerian fruits using spectrophotometric method

Bangladesh Journal of Scientific and Industrial Research ◽

10.3329/bjsir.v52i2.32940 ◽

2017 ◽

Vol 52 (2) ◽

pp. 153-158 ◽

Cited By ~ 3

Author(s):

SO Aremu ◽

CC Nweze

Keyword(s):

Vitamin A ◽

Quantitative Determination ◽

Spectrophotometric Method ◽

Fruits And Vegetables ◽

Significant Difference ◽

Conversion Formula ◽

Major Nutrients

The vitamin A content of the fresh fruits was determined by the extraction and quantitative determination of the pro-vitamin A carotenoid-?-carotene. The Retinol Equivalent (RE) was then obtained using standard conversion formula. The result indicated that the fruit with the minimum vitamin A content and percentage among the six samples was found in mango 301.61 ± 1.03 (7%), which was followed closely by watermelon 350.12 ± 19.01 (8%), guava 504.1 ± 0.75 (11%), tomato 542.86 ± 20.20 (12%), pawpaw 683.93 ± 5.15 (16%) and the highest content was found in carrot 2054.10 ± 1.28 (46%) in (RE/100 g). Statistical calculations and analysis showed that there was significant difference in vitamin A content among the fruits (p<0.05). Thus, besides major nutrients fruits and vegetables contribute very good amount of micro nutrient-vitamin A from ?-carotene.Bangladesh J. Sci. Ind. Res. 52(2), 153-158, 2017

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