Fluorescence intensity and anisotropy decay of the 4?,6-diamidino-2-phenylindole-DNA complex resulting from one-photon and two-photon excitation

1992 ◽  
Vol 2 (2) ◽  
pp. 117-122 ◽  
Author(s):  
Joseph R. Lakowicz ◽  
Ignancy Gryczynski
Keyword(s):  
Fluorescence Intensity ◽  
Two Photon ◽  
Photon Excitation ◽  
Anisotropy Decay ◽  
Dna Complex ◽  
Two Photon Excitation

scholarly journals Time-resolved fluorescence intensity and anisotropy decays of 2,5-diphenyloxazole by two-photon excitation and frequency-domain fluorometry

1992 ◽  
Vol 96 (7) ◽  
pp. 3000-3006 ◽  
Author(s):  
J. R. Lakowicz ◽  
I. Gryczynski ◽  
Z. Gryczynski ◽  
E. Danielsen ◽  
M. J. Wirth
Keyword(s):  
Frequency Domain ◽  
Fluorescence Intensity ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Two Photon ◽  
Photon Excitation ◽  
Anisotropy Decays ◽  
Frequency Domain Fluorometry ◽  
Two Photon Excitation

Exploring the Nature of Photo-Damage in Two-photon Excitation by Fluorescence Intensity Modulation

2008 ◽  
Vol 19 (2) ◽  
pp. 381-386 ◽  
Author(s):  
Arijit Kumar De ◽  
Debabrata Goswami
Keyword(s):  
Fluorescence Intensity ◽  
Intensity Modulation ◽  
Two Photon ◽  
Photon Excitation ◽  
Photo Damage ◽  
Two Photon Excitation

Two-photon excitation microscopy in cellular biophysics

1995 ◽  
Vol 53 ◽  
pp. 62-63
Author(s):  
David W. Piston ◽  
Brian D. Bennett ◽  
Robert G. Summers
Keyword(s):  
Confocal Microscopy ◽  
Laser Beam ◽  
Duty Cycle ◽  
Excited Electronic State ◽  
Input Power ◽  
Two Photon ◽  
Simultaneous Absorption ◽  
Photon Excitation ◽  
Very High ◽  
Two Photon Excitation

Two-photon excitation microscopy (TPEM) provides attractive advantages over confocal microscopy for three-dimensionally resolved fluorescence imaging and photochemistry. Two-photon excitation arises from the simultaneous absorption of two photons in a single quantitized event whose probability is proportional to the square of the instantaneous intensity. For example, two red photons can cause the transition to an excited electronic state normally reached by absorption in the ultraviolet. In practice, two-photon excitation is made possible by the very high local instantaneous intensity provided by a combination of diffraction-limited focusing of a single laser beam in the microscope and the temporal concentration of 100 femtosecond pulses generated by a mode-locked laser. Resultant peak excitation intensities are 106 times greater than the CW intensities used in confocal microscopy, but the pulse duty cycle of 10-5 maintains the average input power on the order of 10 mW, only slightly greater than the power normally used in confocal microscopy.

Two-photon excitation fluorescence microscopy in living systems

1993 ◽  
Vol 51 ◽  
pp. 154-155 ◽  
Author(s):  
David W. Piston
Keyword(s):  
Confocal Microscopy ◽  
Fluorescence Microscopy ◽  
Singlet State ◽  
Excited Electronic State ◽  
Input Power ◽  
Two Photon ◽  
Simultaneous Absorption ◽  
Photon Excitation ◽  
Very High ◽  
Two Photon Excitation

Two-photon excitation fluorescence microscopy provides attractive advantages over confocal microscopy for three-dimensionally resolved fluorescence imaging. Two-photon excitation arises from the simultaneous absorption of two photons in a single quantitized event whose probability is proportional to the square of the instantaneous intensity. For example, two red photons can cause the transition to an excited electronic state normally reached by absorption in the ultraviolet. In our fluorescence experiments, the final excited state is the same singlet state that is populated during a conventional fluorescence experiment. Thus, the fluorophore exhibits the same emission properties (e.g. wavelength shifts, environmental sensitivity) used in typical biological microscopy studies. In practice, two-photon excitation is made possible by the very high local instantaneous intensity provided by a combination of diffraction-limited focusing of a single laser beam in the microscope and the temporal concentration of 100 femtosecond pulses generated by a mode-locked laser. Resultant peak excitation intensities are 106 times greater than the CW intensities used in confocal microscopy, but the pulse duty cycle of 10−5 maintains the average input power on the order of 10 mW, only slightly greater than the power normally used in confocal microscopy.

Two‐photon excitation fluorescence microscopy using a semiconductor laser

Bioimaging ◽  
1995 ◽  
Vol 3 (2) ◽  
pp. 70-75 ◽  
Author(s):  
Pekka E Hänninen ◽  
Martin Schrader ◽  
Erkki Soini ◽  
Stefan W Hell
Keyword(s):  
Fluorescence Microscopy ◽  
Semiconductor Laser ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation
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